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Resonant auger decay of above-threshold core-excited H2O

The decay properties of an above-threshold resonance in core-ionized water, which is difficult to. identify with usual spectroscopic methods, have been analyzed by resonant Auger spectroscopy. The resonance is. shown to correspond to a doubly excited neutral state embedded in the O 1s ionization continuum. The main, result of the experiments is the assessment on the dissociative nature of the inte

A novel pattern of pp65-positive cytomegalic endothelial cells circulating in peripheral blood from a renal transplant recipient

The present study reports a novel pattern of cytomegalic endothelial cells (CEC) in peripheral blood from a female renal transplant recipient infected with human cytomegalovirus (HCMV), which has not been reported previously. Localization of specific early antigen of HCMV, pp65 antigen, was examined by immunohistochemistry. Staining of an endothelial cell marker (CD34) was used to characterize end

Activation of type I interferon system in systemic lupus erythematosus correlates with disease activity but not with antiretroviral antibodies

The objective was to investigate the relation between serum levels of interferon-alpha (IFN-alpha), the activity of an endogenous IFN-alpha inducing factor (SLE-IIF), clinical and immunological disease activity as well as serum levels of antiretroviral antibodies in SLE. Serum levels of IFN-alpha were measured in serial sera from 30 patients sampled at different stages of disease activity (SLEDAI

Attractors in continuous and Boolean networks

We study the stable attractors of a class of continuous dynamical systems that may be idealized as networks of Boolean elements, with the goal of determining which Boolean attractors, if any, are good approximations of the attractors of generic continuous systems. We investigate the dynamics in simple rings and rings with one additional self-input. An analysis of switching characteristics and puls

Formation and conversion of oxygen metabolites by Lactococcus lactis subsp lactis ATCC 19435 under different growth conditions

A semidefined medium based on Casamino Acids allowed Lactococcus lactis ATCC 19435 to grow in the presence of oxygen at a slow rate (0.015 h(-1)). Accumulation of H2O2 in the culture prevented a higher growth rate. Addition of asparagine to the medium increased the growth rate; whereby H2O2 accumulated only temporarily during the lag phase. H2O2 is an inhibitor for several glycolytic enzymes, glyc

Expression of matrix metalloproteinase-2 and mutant p53 is increased in hydatidiform mole as compared with normal placenta

Matrix metalloproteinases (MMPs) are group of enzymes thought to play an important role in trophoblastic and tumor invasion. The aim of our study was to investigate the trophoblastic expression of MMPs and p53 in normal trophoblast and hydatidiform moles (HM). Paraffin sections of 45 specimens, including 14 complete hydatidiform moles (CM), 15 partial hydatidiform moles (PM), 8 atypical partial hy

Production of external mycelium by ectomycorrhizal fungi in a Norway spruce forest was reduced in response to nitrogen fertilization

A field study was carried out to evaluate the influence of N fertilization on the growth of the external mycelium of ectomycorrhizal (EM) fungi in a Norway spruce forest in SW Sweden. Nylon mesh bags filled with sand were buried in the soil for 6-18 months and the ingrowth of mycelium was used as an estimate of EM mycelial growth. Root-isolated, trenched plots were used to estimate background grow

The role of advective fluid flow and diffusion during localized, solid-state dehydration: Sondrum Stenhuggeriet, Halmstad, SW Sweden

A localized dehydration zone, Sondrum stone quarry, Halmstad, SW Sweden, consists of a central, 1 m wide granitic pegmatoid dyke, on either side of which extends a 2.5-3 m wide dehydration zone (650-700 degrees C; 800 MPa; orthopyroxene-clinopyroxene-biotite-amphibole-garnet) overprinting a local migmatized granitic gneiss (amphibole-biotite-garnet). Whole-rock chemistry indicates that dehydration

Gravimetric antigen detection utilizing antibody-modified lipid bilayers

Lipid bilayers containing 5% nitrilotriacetic acid (NTA) lipids supported on SiO2 have been used as a template for immobilization of oligohistidine-tagged single-chained antibody fragments (scFvs) directed against cholera toxin. It was demonstrated that histidine-tagged scFvs could be equally efficiently coupled to an NTA-Ni2+-containing lipid bilayer from a purified sample as from an expression s

A novel mechanism for protein delivery: granzyme B undergoes electrostatic exchange from serglycin to target cells

The molecular interaction of secreted granzyme B-serglycin complexes with target cells remains undefined. Targets exposed to double-labeled granzyme B-serglycin complexes show solely the uptake of granzyme B. An in vitro model demonstrates the exchange of the granzyme from serglycin to immobilized, sulfated glycosaminoglycans. Using a combination of cell binding and internalization assays, granzym

Early and late invasive pneumococcal infection following stem cell transplantation: a European Bone Marrow Transplantation survey

Streptococcus pneumoniae (S. pneumoniae) may cause severe and lethal infections months and years following stem cell transplantation (SCT). In a prospective survey over a 3.5-year period, we assessed the incidence, risk factors and outcome for invasive pneumococcal infection (IPI) following SCT. Fifty-one episodes of IPI were reported: 43 episodes after bone marrow transplantation (BMT) and 8 afte

Risk and case characteristics of tuberculosis in rheumatoid arthritis associated with tumor necrosis factor antagonists in Sweden

Objective. Because treatment with tumor necrosis factor (TNF) antagonists may increase the risk of tuberculosis (TB), and because knowledge of the risk of TB in rheumatoid arthritis (RA) not treated with biologics is scarce and of uncertain generalizability to low-risk populations, this study sought to determine the risk of TB among Swedish patients with RA. Methods. Using data from Swedish nation

Tri-trophic effect on predator feeding: consumption by the carabid Harpalus affinis of Heliothis armigera caterpillars fed on proteinase inhibitor-containing diet

During the breeding of our major crop plants for high yield and edibility, several desirable traits, including resistance to pest insects, have been lost (Gatehouse et al., 1991). Genetic engineering is now vigorously seeking to reinsert such genes into crop plants, making them toxic or less palatable for herbivorous pests. One class of inhibitors under study are protease/proteinase inhibitors. Th

Electrochemical switching of the flavoprotein dodecin at gold surfaces modified by flavin-DNA hybrid linkers

Dodecin from Halobacterium salinarum is a dodecameric, hollow-spherical protein, which unspecifically adopts flavin molecules. Reduction of flavin dodecin holocomplexes induces dissociation into apododecin and free flavin. Unspecific binding and dissociation upon reduction were used as key properties to construct an electrochemically switchable surface, which was able to bind and release dodecin a

Generation of molecular recognition sites in electrospun polymer nanofibers via molecular imprinting

A simple method for the formation of molecular recognition sites in polymer nanofibers was developed. Electrospun nanofibers were prepared from a Solution mixture of poly(ethylene terephthalate) (PET) and polyallylamine in the presence of a template molecule, 2,4-dichlorophenoxyacetic acid (2,4-D). The polyamine was used to provide functional groups that interact with the template during the elect

A sequential logic device realized by integration of in-plane gate transistors in InGaAs/InP

An integrated nanoelectronic circuit is fabricated from a high-mobility In0.75Ga0.25As/InP heterostructure. The manufactured device comprises two double in-plane gate transistors with a current channel of 1.1 mu m in length and 100 nm in width. The two transistors are coupled to each other in a configuration that the source of one transistor is directly connected with one in-plane gate of the othe

Palaeomagnetic intensity data: An Achilles heel of solar activity reconstructions

Reconstructions of solar activity prior to AD1600 rely on estimates of the past production rates of cosmogenic radionuclides in the Earth’s atmosphere. These rates are modulated by the strength of the solar magnetic field and the intensity of the Earth’s geomagnetic dipole. One of the largest uncertainties associated with these reconstructions originates from palaeointensity data and to show how e

Epithelial and ectomesenchymal role of the type I TGF-beta receptor ALK5 during facial morphogenesis and palatal fusion

Transforming growth factor beta (TGF-beta) proteins play important roles in morphogenesis of many cramofacial tissues; however, detailed biological mechanisms of TGF-beta action, particularly in vivo, are still poorly understood. Here, we deleted the TGF-beta type I receptor gene Alk5 specifically in the embryonic ectodermal and neural crest cell lineages. Failure in signaling via this receptor, e