Protein Stability, Folding and Design: Stabilization of scFv Protein through its reconstitution using a split GFB biosensor in vivo
General rules for rational design as well as prediction of tertiary structure and functionality of a protein can be described by investigating the interactions and the role of particular amino acids in protein structure. Mutagenesis has been used commonly to generate stable variants, with an ultimate goal to unravel the rules of protein stability and folding. Besides, reconstitution of dissected p