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Sloped side walls in SU-8 structures with ‘Step-and-Flash’ processing

We present a fast fabrication technique to achieve SU-8 structures with sloped side walls. We investigate the method of ‘Step-and-Flash’ which combines hot embossing with UV sensitive materials. The stamps are fabricated in Si by KOH etching to achieve mesa structures with side walls at a 54.7° angle to the bottom. These stamps are imprinted into SU-8-coated Pyrex wafers during the soft bake step

Cytological variation and pathogenicity of the bumble bee parasite Nosema bombi (Microspora, Nosematidae)

In three field seasons, 2003-2005. bumble bees were collected in southern Sweden and eastern Denmark in search of microsporidian parasites. Of file 16 bumble bee species studied, microsporidia were found in Bomhus hortorum, Bomhus hypnorum, Bombas lapidarius, Bomus lucorum, Bombus pascuorum, Bombus pratorum, Bombus ruderarius, and Bombus terrestris. Only one micro-sporidian species, Nosema bombi,

Optimization of steam pretreatment of SO2-impregnated corn stover for fuel ethanol production

In this study, corn stover with a dry matter content of 20% was impregnated with SO2 and then steam pretreated for various times at various temperatures. The pretreatment was evaluated by enzymatic hydrolysis of the solid material and analysis of the sugar content in the liquid. The maximum overall yield of glucose, 89% of the theoretical based on the glucan in the raw material, was achieved when

In vitro fertilization (IVF) in Sweden: Risk for congenital malformations after different IVF methods.

BACKGROUND: The possible excess of congenital malformations in infants born after in vitro fertilization (IVF) has been much discussed in the literature, with controversial conclusions. This population based study is aimed at analyzing the presence of congenital malformations in a large group of infants born after IVF and to compare malformation risk both with that of all infants born and accordin

Acute confusional episodes in elderly orthopaedic patients: the patients' actions and speech.

The aim of this study was to illuminate the pattern of confusional episodes with regard to the content of elderly patients' actions and speech. Fifty-one (11.2%) out of 457 people admitted to an orthopaedic clinic developed acute confusional state. Non-participant direct observations and daily clinical bedside follow-up observations were made during the confusional episodes, focusing on the patien

Central and mixed venous blood oxygen correlate well during acute normovolemic hemodilution in anesthetized pigs

BACKGROUND: Central venous oxygen saturation (ScvO2) and oxygen tension (PcvO2), obtained from the superior vena cava, correlate well with mixed venous (pulmonary arterial) oxygen saturation (SvO2) and tension (pvO2) when the hematocrit is normal. The present study was undertaken to assess whether extreme hemodilution affects this relation. METHODS: We compared mixed and central venous blood durin

Mechanisms of adaptation to the effects of ethanol on activation of phospholipase C in NG 108-15 cells

In this study the effect of different times of exposure to ethanol (1-7 days, 100 mM) on bradykinin and GTP(S)-stimulated activation of phospholipase C in NG 108-15 cells and on the binding of [3H]bradykinin to its receptors was investigated. Ethanol attenuated both agonist and GTP-analogue-induced hydrolysis of phosphoinositides for a period of up to 4 days of treatment, while exerting no effect

Predictions of local ground geomagnetic field fluctuations during the 7-10 November 2004 events studied with solar wind driven models

The 7-10 November 2004 period contains two events for which the local ground magnetic field was severely disturbed and simultaneously, the solar wind displayed several shocks and negative B-z periods. Using empirical models the 10-min RMS Delta X and Delta Y at Brorfelde (BFE, 11.67 degrees E, 55.63 degrees N), Denmark, are predicted. The models are recurrent neural networks with 10-min solar wind

Highly sequence-specific binding is retained within the DNA-binding domain of the Saccharomyces castellii Cdc13 telomere-binding protein

The essential protein Cdc13p binds the single-stranded telomeric 3' overhangs in Saccharomyces cerevisiae and takes part in the regulation of telomere length. The DNA-binding domain (DBD) of Cdc13p is structurally established by an oligonucleotide/oligosaccharide-binding (OB)-fold domain. The sequence homolog in Saccharomyces castellii (scasCDC13) was characterized previously, and the full-length

Structure-function studies of the C3a-receptor: C-terminal serine and threonine residues which influence receptor internalization and signaling

The anaphylatoxic peptide C3a is a pro-inflammatory mediator generated during complement activation, whose specific G protein coupled receptor is expressed on granulocytes, monocytes, mast cells, activated lymphocytes, and in the nervous tissue. We have generated RBL-2H3 cell clones stably expressing mutants of the human C3a-receptor (C3aR) with combined alanine (Ala) substitutions of ten C-termin

Periarticular tissue levels of cytokine- and endothelin-1-like immunoreactivity during the course of antigen-induced arthritis in the rat

OBJECTIVE AND DESIGN: To correlate the changes in periarticular tissue levels of pro-inflammatory cytokines and endothelin-1 (ET-1) to local pathophysiology in rats with antigen-induced arthritis (AIA). MATERIALS: The periarticular soft tissue was excised from sixty-six rats at different stages of AIA. METHODS: The samples were homogenized and the levels of immune like (LI) reactivities were deter