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The homeodomain transcription factors Islet 1 and HB9 are expressed in adult alpha and gamma motoneurons identified by selective retrograde tracing

To study gene expression in differentiated adult motoneuron subtypes, we used fluorescent dextrans for both anterograde and retrograde axonal tracing in adult rat and mouse. Application of these dyes to the cut distal and proximal ends of small extramuscular nerve branches revealed both the peripheral ramifications and the cell bodies of subsets of motoneurons. We show that the soleus muscle is in

Fluorescence studies on the interaction between pyrene-labelled poly(acrylic acid) and cyclodextrins

The interactions, in aqueous media, between a pyrene-labelled polyelectrolyte poly(acrylic acid) (PAAMePy) with two different degrees of labelling and beta- and gamma-cyclodextrins (beta- and gamma-CD) were studied using absorption and fluorescence (steady-state and time-resolved) techniques. In addition to qualitative and quantitative parameters obtained from absorption and steady-state fluoresce

Engineered multidomain cysteine protease inhibitors yield resistance against western flower thrips (Franklinielia occidentalis) in greenhouse trials

Western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripiclae), cause very large economic damage on a variety of field and greenhouse crops. In this study, plant resistance against thrips was introduced into transgenic potato plants through the expression of novel, custom-made, multiclomain protease inhibitors. Representative classes of inhibitors of cysteine and aspartic

Strategies Applied by Women in Coping With ad-hoc Demands for Unauthorized User Fees During Pregnancy and Childbirth. A Focus Group Study From Angola.

Our aim in this study was to explore women's responses to reported ad-hoc demands for unauthorized user fees during pregnancy and childbirth in Luanda, Angola. Ten focus group discussions were conducted and data were analysed using grounded theory. Women were found to apply six strategies as they "endeavored to cope with demands for unauthorized user fees" (core category): (i) blowing the whistle,

Effects of sensor, trials and knee joint variables on electrogoniometric gait recordings

Introduction: Different sources of variations, such as electrogoniometer characteristics and procedures, may affect the accuracy and precision of movement measurements during gait. Objective: To quantify the variations and compare the effects produced by different sources of variation in electrogoniometric gait recordings: the sensors, procedures (trials) and the knee joint. Methods: Knee flexion/

Anaerobic biodegradation of spent sulphite liquor in a UASB reactor

Anaerobic biodegradation of fermented spent sulphite liquor, SSL, which is produced during the manufacture of sulphite pulp, was investigated. SSL contains a high concentration of lignin products in addition to hemicellulose and has a very high COD load (173 g COD 1(-1)). Batch experiments with diluted SSL and pretreated SSL indicated a potential of 12-22 1 methane per hire SSL, which corresponds

Ethanol Solvation in Water Studied on a Molecular Scale by Photoelectron Spectroscopy

Because of the amphiphilic properties of alcohols, hydrophobic hydration is important in the alcohol-water system. In the present paper we employ X-ray photoelectron spectroscopy (XPS) to investigate the bulk and surface molecular structure of ethanol-water mixtures from 0.2 to 95 mol %. The observed XPS binding energy splitting between the methyl C 1s and hydroxymethyl C 1s groups (BES-[CH3-CH2OH

A new analytical method for lead determination in atmospheric particulate matter by a combination of ultrasound-assisted extraction and supramolecular solvent preconcentration

Air pollution by lead, even in small concentrations, can cause serious health effects. In the atmosphere this element can generally be found in particulate matter (PM), and in general in small concentrations. This work proposes the development of an analytical method for lead determination in inhaled PM based on ultrasound-assisted extraction with subsequent supramolecular solvent based dispersive

Accuracy and precision of statistical descriptors obtained from multidimensional diffusion signal inversion algorithms

In biological tissues, typical MRI voxels comprise multiple microscopic environments, the local organization of which can be captured by microscopic diffusion tensors. The measured diffusion MRI signal can, therefore, be written as the multidimensional Laplace transform of an intravoxel diffusion tensor distribution (DTD). Tensor-valued diffusion encoding schemes have been designed to probe specif

Proteoglycans from chick limb bud chondrocyte cultures. Keratan sulfate and oligosaccharides which contain mannose and sialic acid

The precursors, [ 35S]sulfate and [2- 3H]mannose, were used to study the biosynthesis of keratan sulfate and other oligosaccharides on proteoglycans isolated from Day 8 cultures of chick limb bud chondrocytes. After alkaline borohydride treatment, three fractions with sialic acid were separated by molecular sieve chromatography. The first contained keratan sulfate which was purified by digestion w

Symbol time offset estimation in coherent OFDM systems

This paper presents a symbol time offset estimator for coherent OFDM systems. This estimator exploits both the redundancy in the cyclic prefix and the pilot symbols used for channel estimation. The estimator is robust against frequency offsets and is suitable for use in dispersive channels. We base the estimator on the ML estimator for the AWGN channel. Simulations of an example system indicate a

EFFECT OF THE FIELD MAPS ON THE BEAM DYNAMICS OF THE ESS DRIFT TUBE LINAC

In the beam dynamics design and modelling of the Eu- ropean Spallation Source (ESS) Drift Tube Linac (DTL) simplified models have been used for the focusing and ac- celerating elements. Since the high current requires precise control of the beam to analyze the losses it is useful to per- form the beam dynamics simulations by using accurate field maps of the focusing and accelerating elements. In t

Godtyckligt regelverk hotar friheten på nätet

Reglerna som möjliggör stängning av hemsidor på internet präglas av godtycke och otydlighet. Men det behöver inte vara särskilt svårt att skapa ett nytt och rättssäkert regelverk. Här har Sveriges EU-kommissionär Cecilia Malmström en viktig roll. Frågan är om hon tar sitt ansvar, skriver politiker och nätdebattörer.

European Spallation Source Lattice Design Status

The accelerator of the European Spallation Source (ESS) will deliver 62.5 mA proton beam of 2.0 GeV onto the target, offering an unprecedented beam power of 5 MW. Since the technical design report (TDR) was published in 2013, work has continued to further optimise the accelerator design. We report on the advancements in lattice design optimisations after the TDR to improve performance and flexibil