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Cloning and characterisation of Aspergillus niger genes encoding an alpha-galactosidase and a beta-mannosidase involved in galactomannan degradation

α-Galactosidase (EC 3.2.1.22) and β-mannosidase (EC 3.2.1.25) participate in the hydrolysis of complex plant saccharides such as galacto(gluco)mannans. Here we report on the cloning and characterization of genes encoding an α-galactosidase (AglC) and a β-mannosidase (MndA) from Aspergillus niger. The aglC and mndA genes code for 747 and 931 amino acids, respectively, including the eukaryotic signa

Anatomy and biomechanics of the back muscles in the lumbar spine with reference to biomechanical modeling

Study Design. This article describes the development of a musculoskeletal model of the human lumbar spine with focus on back muscles. It includes data from literature in a structured form. Objective. To review the anatomy and biomechanics of the back muscles related to the lumbar spine with relevance for biomechanical modeling. Summary of Background Data. To reduce complexity, muscle units have be

Radio-telemetry observations of the first 650 km of the migration of Bar-tailed Godwits Limosa lapponica from the Wadden Sea to the Russian Arctic

In 1999 and 2000, 45 Bar-tailed Godwits Limosa lapponica were supplied with radio-transmitters during spring staging on the island Texel in the western Wadden Sea. With the use of Automatic Radio Tracking Stations (ARTS) on Texel and in south Sweden, and hand-held receivers on Texel, it was possible to follow the later part of the stopover period on Texel for 34 birds (76%) and the passage over so

Boris Lurie and NO!art

Article about the Latvian-American artist Boris Lurie, the art movement NO!art, founded by Lurie, Sam Goodman and Stanley Fischer in 1959 in New York, and the reception history of Lurie and NO!art from the 1960s until today. Accompanied by an extensive interview with Boris Lurie.

OFDM channel estimation by singular value decomposition

We present and analyze low-rank channel estimators for orthogonal frequency-division multiplexing (OFDM) systems using the frequency correlation of the channel. Low-rank approximations based on the discrete Fourier transform (DFT) have been proposed, but these suffer from poor performance when the channel is not sample spaced. We apply the theory of optimal rank-reduction to linear minimum mean-sq

Improving initial xylose metabolism in recombinant Saccharomyces cerevisiae

The aim of the thesis is to improve the initial steps of xylose metabolism in recombinant Saccharomyces cerevisiae. S. cerevisiae takes up xylose of poor affinity by means of hexose transporters. Metabolic control analysis was used to investigate whether the low xylose utilisation rate is due to inefficient transport. Xylose transport was found to have little control over the aerobi

Governance Challenges for Greening the Urban Economy: Understanding and Assessing the Links between Governance and Green Economy in Cities

The challenges for creating a greener economy and the institutional framework for sustainable development pass necessarily, or mostly, through cities, as they concentrate a large and growing part of the world’s economy and population, as well as decision-making power. With the processes of urbanization and rural-urban transformation, the economy in cities, especially in cities of developing countr

In vivo and in vitro intragenomic rearrangement of TT viruses

The in vitro replication of the Torque teno virus (IT virus) tth8 full-length genome and particle formation in a Hodgkin's lymphoma-derived cell line after transfection with cloned viral DNA were demonstrated. Analyses of the transcription patterns of tth8 and tth7 TT virus isolates in a number of lymphoma and T-cell leukemia cell lines indicated differential additional splicing events and intrage

U-Pb geochronology and paleomagnetism of the Westerberg Sill Suite, Kaapvaal Craton - Support for a coherent Kaapvaal-Pilbara Block (Vaalbara) into the Paleoproterozoic?

Precise geochronology, combined with paleomagnetism on mafic intrusions, provides first-order information for paleoreconstruction of crustal blocks, revealing the history of supercontinental formation and break-up. These techniques are used here to further constrain the apparent polar wander path of the Kaapvaal Craton across the Neoarchean-Paleoproterozoic boundary. U-Pb baddeleyite ages of 2441

Comparison of genotyping methods by application to Salmonella livingstone strains associated with an outbreak of human salmonellosis

During 2000 and 2001, an outbreak of human salmonellosis occurred in Sweden and Norway, caused by Salmonella livingstone. In this study, the genotypic differences between three strains obtained from food sources during the outbreak, two human strains and 27 more or less unrelated strains were analysed, using the three methods; automated ribotyping, pulsed field get electrophoresis (PFGE) and rando

Blue straggler production in globular clusters

Recent Hubble Space Telescope observations of a large sample of globular clusters reveal that every cluster contains between 40 and 400 blue stragglers.The population does not correlate with either stellar collision rate (as would be expected if all blue stragglers were formed via collisions) or total mass (as would be expected if all blue stragglers were formed via the unhindered evolution of a s

Bezafibrate-induced anaphylactic shock: unusual clinical presentation

We report a case of a patient who suffered generalized urticaria, chest tightness, wheezing, nausea, vomiting, hypotension, and loss of consciousness. Two hours earlier she had taken Eulitop Retard following lunch. She had tolerated all the implicated food after the reaction. Allergy evaluation revealed intense positive responses to intradermal tests with bezafibrate active component and Eulitop R