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Determination of 3′-phosphoadenosine-5′-phosphosulfate in cells and Golgi fractions using hydrophilic interaction liquid chromatography–mass spectrometry

3′-Phosphoadenosine-5′-phosphosulfate (PAPS) is a key player in the sulfation of biomolecules, but methods for selective measurements are lacking. A liquid chromatography–mass spectrometry (LC–MS) approach for measuring PAPS was developed. A central feature of the method was employing hydrophilic interaction liquid chromatography (HILIC), which is highly suited for separating very polar/charged co

Filmstjärnan var tekniskt geni

Integrative experimental characterization and engineering modeling of single-dowel connections in LVL

In order to be able to realistically and consistently elucidate and subsequently simulate the load–displacement behavior of single-dowel connections, the material behavior of the individual components, namely steel dowels and wood, needs to be investigated. The behavior of slotted-in, single-dowel steel-to-laminated veneer lumber (LVL) connections with dowel diameters of 12 and 20 mm is thoroughly

Såldes som får - blev en av USA:s största hjältar

Hans osäljbara verk blev dyrgripar

Världens första programmerare

Strategies and organisational measures for a sustainable working life to an older age

Varför får vi inte se museernas samlingar?

Våra ljus i mörkret skriver historia

Årets Böcker : Historia

Britannia : en lång historisk parentes

A 3.5-6.8GHz wide-bandwidth DTC-assisted fractional-N all-digital PLL with a MASH ΔΣ TDC for low in-band phase noise

We present a digital-to-time converter (DTC)-assisted fractional-N wide-bandwidth all-digital PLL (ADPLL). It employs a MASH ΔΣ time-to-digital converter (TDC) to achieve low in-band phase noise, and a wide-tuning range digitally-controlled oscillator (DCO). Fabricated in 40nm CMOS, the ADPLL consumes 10.7 mW while outputting 1.73 to 3.38 GHz (after a ÷2 division) and achieves better than -109 dBc

Prevalence and Prognostic Implications of Bundle Branch Block in Comatose Survivors of Out-of-Hospital Cardiac Arrest

This study reports the prevalence and prognostic impact of right bundle branch block (RBBB) and left bundle branch block (LBBB) in the admission electrocardiogram (ECG) of comatose survivors of out-of-hospital cardiac arrest (OHCA). The present study is part of the predefined electrocardiographic substudy of the prospective randomized target temperature management trial, which found no benefit of

Is age and ageing important for extending working life? Conceptualisation of ageing in relation to factors in working life

Gender and Work Life Exits

Dime Novels för flickor i USA vid 1800-talets slut

Has the older employees in the workforce increased? Study of the total Swedish population regarding exit from working life

Above and belowground responses of four tundra plant functional types to deep soil heating and surface soil fertilization

A simplified model to estimate the concentration of inorganic ions and heavy metals in rivers

This paper presents a model that uses only pH, alkalinity, and temperature to estimate the concentrations of major ions in rivers (Na+, K+, Mg2+, Ca2+, HCO3 -, SO4 2-, Cl-, and NO3 -) together with the equilibrium concentrations of minor ions and heavy metals (Fe3+, Mn2+, Cd2+, Cu2+, Al3+, Pb2+, and Zn2+). Mining operations have been increasing, which has led to changes in the pollution loads to r

Designing monoclonal antibody fragment-based affinity resins with high binding capacity by thiol-directed immobilisation and optimisation of pore/ligand size ratio

Monoclonal antibody (mAb) based affinity resins usually suffer from low binding capacity, most probably as a result of steric hindrance by the large 150 kDa size of the mAb and a random immobilisation approach. The present work investigates the influence of a variety of factors on dynamic binding capacity (DBC) such as pore/ligand size ratio, accessibility of ligand and ligand density. The effect