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A microarray approach for identifying mutated genes

This study was performed to evaluate if microarray technology can identify genes using their transcriptionally defective mutant alleles. Three barley (Hordeum vulgare L.) mutant strains, xantha-h57, xantha-f27 and xantha-g28, with mutations in the genes encoding the three subunits of the chlorophyll biosynthetic enzyme magnesium chelatase, were used in a reconstruction experiment. The mutation xan

Are important patient-rated outcomes in community mental health care explained by only one factor?

Introduction: The study tested whether four commonly used patient-rated outcomes are explained by only one factor, reflecting a general appraisal tendency of patients. Method: Quality of life, needs and symptoms were rated by 92 patients in community mental health care at baseline and after 18 months and 6 years follow-up periods. At follow ups treatment satisfaction was also assessed. Scores and

Evaluation of endometrial receptivity during in-vitro fertilization using three-dimensional power Doppler ultrasound

Objective To compare sonographic endometrial characteristics in in-vitro fertilization (IVF) cycles between women who conceive and those who do not. Methods Thirty-five women undergoing IVF treatment participated in the study. Using three-dimensional (3D) power Doppler ultrasound, we assessed endometrial patterns, volume and vascularization, after follicle stimulating hormone (FSH) stimulation but

Paired multiplex reverse-transcriptase polymerase chain reaction (PMRT-PCR) analysis as a rapid and accurate diagnostic tool for the detection of MLL fusion genes in hematologic malignancies

The MLL gene in chromosome band 11q23 is frequently rearranged in acute lymphoblastic and acute myeloid leukemias. To date, more than 50 different chromosomal regions are known to participate in translocations involving 11q23, many of which affect MLL. The pathogenetically important outcome of these rearrangements is most likely the creation of a fusion gene consisting of the 5' part of the MLL ge

Short hospital stay augmented with education and home-based rehabilitation improves function and quality of life after hip replacement - Randomized study of 50 patients with 6 months of follow-up

Background Because of current cost restrictions, we studied the effect of a shorter hospital stay on function, pain and quality of life (QOL) after total hip replacement (THR). Patients and methods 50 patients from two hospitals were randomized into a study group (SG) of 27 patients receiving preoperative and postoperative education programs, as well as home visits from an outpatient team, and a c

Inter-tester reliability of a new diagnostic classification system for patients with non-specific low back pain

Most patients referred to physiotherapy with low back pain are without a precise medical diagnosis. Identification of subgroups of non-specific low back pain patients may improve clinical outcomes and research efficiency. A pathoanatomic classification system has been developed, classifying patients with non-specific low back pain into 12 different syndromes and three subcategories based on histor

Hearing thresholds in four-year-old children with weak or no transient-evoked otoacoustic emissions

Play audiometry is part of the general developmental screening covering 4-year-old children in the city of Malmo (Sweden). In an open study, 10% of the cohort was tested with transient-evoked otoacoustic emissions (TEOAEs), using the ILO88 system. The aim was to reveal a hearing loss exceeding 25 dB HL in 4-year-old children. As the first step in determining whether emissions can be used as an eff

Identification of the major proteoglycans from human myometrium

Background. During pregnancy and parturition a remodeling within the extracellular matrix of the cervix and the corpus uteri occurs, which is of fundamental importance to a normal labor. The aim of this study is to identify the major proteoglycans in corpus uteri of non-pregnant subjects. Methods. From human uterine tissue proteoglycans were extracted and purified using CsCl-density gradient centr

High-resolution In-beam Particle Spectroscopy - New Results on Prompt Proton Emission from 58Cu

Prompt proton decay lines in Cu-58 have been studied by means of high-resolution in-beam particle-gamma coincidence spectroscopy using the GAMMASPHERE Ge-detector array in conjunction with a dedicated set of ancillary detectors including four DeltaE-E silicon-strip telescopes. High-spin states in Cu-58 have been populated via the heavy-ion fusion-evaporation reaction Si-28(Ar-36, 1alpha1p1n) at 14

Evidence for noncollective oblate structures at high spin in Cs-123

High-spin states in Cs-123 were populated in the Ni-64(Ni-64,p4n) reaction at a beam energy of 265 MeV. Gamma-ray coincidences were measured using the Gammasphere spectrometer. Two additional bands have been placed in the level scheme and the four previously known bands have been extended to higher spin. At the highest spins, two of the bands show irregular level sequences. These structures of non

Hyaluronan is organized into fiber-like structures along migratory pathways in the developing mouse cerebellum

Hyaluronan is a free glycosaminoglycan which is abundant in the extracellular matrix of the developing brain. Although not covalently linked to any protein it can act as a backbone molecule forming aggregates with chondroitin sulfate proteoglycans of the lectican family and link proteins. Using neurocan-GFP as a direct histochemical probe we analyzed the distribution and organization of hyaluronan

Numerical Investigation of Turbulent Fluid Flow and Heat Transfer in Complex Ducts

Popular Abstract in Swedish Numeriska methoder har används för att beräkna turbulent strömning och värmeöverföring i komplexa kanaler med olika turbulens modeller.Th need for a reliable and reasonable accurate turbulence model without specific convergence problem for calculating duct flows in industrial applications has become more evident. In this study a general computational method has been developed for calculating turbulent quantities in any arbitrary three dimensional duct. Four different turbulence models for predicting the turbulent Reynolds stresse