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Nigella damascena seeds were extracted by cold press, in a Soxhlet apparatus and with CO2 The yield obtained with liquid CO2 was only 10.57%. EtOH (1%) increased the yield by 50%. CO2-extracts were separated into the 2 fractions. The yield in the first fraction increased 2 times by increasing the pressure from 150 to 350 bar. EtOH (1%) increased the yield 2 times at 150 bar. Linoleic acid was the

In a 5-y retrospective follow-up study in southern Sweden that initially included > 1000 individuals with previously diagnosed erythema migrans, factors that influenced the risk of reinfection with Lyme borreliosis were elucidated. The total frequency of reinfection was 4% and the number of tick bites influenced the risk of reinfection: those bitten > 10 times during the observation period had a 4

Ribosomal protein S6 kinase (S6K) is activated by an array of mitogenic stimuli and is a key player in the regulation of cell growth. The activation process of S6 kinase involves a complex and sequential series of multiple Ser/Thr phosphorylations and is mainly mediated via phosphatidylinositol 3-kinase (PI3K)-3-phosphoinositide-dependent protein kinase-1 (PDK1) and mTor-dependent pathways. Upstre

Calponin (4.1-5.9 microM, pig stomach) inhibited maximal shortening velocity (Vmax) by 20-25% with only minor influence on force in skinned smooth muscle from guinea-pig taenia coli activated at different Ca2+ levels and with thiophosphorylation. Similar results were obtained with a fragment of the N-terminal 1-228 amino acids engineered using a mouse cDNA construct (5.4 microM). Both the native c

High spin states in the transitional nucleus Nb-87 up to 14 MeV excitation have been established for the first time via the reactions Ca-40(Cr-50, 3p)Nb-87 and Ni-58(S-32, 3p)Nb-87. The Nb-87 gamma-radiations have been identified through gamma-ray spectra taken in coincidence with the evaporation residues detected in the Daresbury recoil separator or with multiple proton emission. Gamma-gamma coin

Abstract is not available

Traditionally, human embryonic stem cells (hESCs) are propagated by mechanical dissection or enzymatic dissociation into clusters of cells. To facilitate up-scaling and the use of hESC in various experimental manipulations, such as fluorescence-activated cell sorting, electroporation, and clonal selection, it is important to develop new, stable culture systems based on single-cell enzymatic propag

AIMS: Although the perpetuation of several supraventricular arrhythmias is critically dependent upon intra-atrial conduction, the literature lacks detailed information on normal values of conduction velocity and degree of anisotropy. In order to explore these factors further, we have measured conduction velocities at the right atrial free wall during sinus rhythm and during atrial pacing in four d

The calpain-10 gene (CAPN10) has been associated with type 2 diabetes, but information on molecular and physiological mechanisms explaining this association is limited. Here we addressed this question by studying the role of CAPN10 for phenotypes associated with type 2 diabetes and free fatty acid (FFA) metabolism. Among 395 type 2 diabetic patients and 298 nondiabetic control subjects from Finlan

Popular Abstract in Swedish Matematiska modeller och simulering är viktiga verktyg för att hantera tekniska system. Trenden idag är att öka komplexiteten i system genom integrerad produktion och processoptimering. Att hantera dessa komplexa system kräver kunskaper inom många områden samtidigt. Modellbibliotek är ett bra sätt att paketera kunskap. Kommersiella simuleringsprogram tillandahåller bra This thesis describes basic models for thermo-fluid systems and the implementation of the models using a flexible object-oriented structure in the ThermoFluid library. Model libraries provide an excellent way to package engineering knowledge of systems to be reused by non-experts. Many commercial simulation packages provide good model libraries, but they are usually domain specific and closed. Mod

Vascular smooth muscle cell migration, occurring after intimal injury, is a substantial clinical problem in atherosclerosis and restenosis after stenting. Here we investigate the effects of polyamine synthesis inhibition on vascular smooth muscle cell migration after maximal and submaximal growth stimulation with PDGF-AB or FCS. Vascular smooth muscle cells were obtained from mouse aorta explants.

The use of surface plasmon resonance (SPR), for the comparison of metal binding properties of polyhistidine tags, was evaluated. Six different tags containing various number of histidines, either none (tags n and t), three (tags H(3)A(3) and H(2)AHA(2)H) or six (tags H-6 and HiS(6)) were genetically fused to the N-terminal of lactate dehydrogenase (LDH). The binding ability of these constructs to

In this paper, we propose a method for suppressing radio frequency interference (RFI) in discrete multitone (DMT) based very high bit rate digital subscriber line (VDSL) systems. The method operates in the frequency domain of a DMT system. First, we derive a model of how an unknown narrow-band RF signal is mapped onto the DMT carriers. Then, by measuring the RFI on a few unused DMT carriers we are

Results from the design of a high-pressure chamber and its components, recently developed at the Lund Institute of Technology, are presented. The results show that at high pressures, when the lubricants have solidified, the density varies linearly with the pressure and the shear strength also increases nearly linearly with pressure. This is also true for the investigated gasses when they are compr

A low molecular weight heparin (LMW-heparin) with a mean molecular weight of 4900 dalton was prepared by controlled enzymatic depolymerization of conventional porcine mucosal heparin. The effects of 2,500, 5,000 and 10,000 U (XaI; 29, 58 and 116 mg) on factor Xa inhibition (XaI), factor IIa inhibition (IIaI), APTT, AT III and platelet count were compared to those of 5,000 U (XaI; 26 mg) of convent

Software inspection is a method to detect faults in the early phases of the software life cycle. In order to estimate the number of faults not found, capture-recapture was introduced for software inspections in 1992 to estimate remaining faults after an inspection. Since then, several papers have been written in the area, concerning the basic theory, evaluation of models and application of the met