Lipases at Solid Surfaces An Adsorption and Activity Study
Popular Abstract in Swedish Lipasadsorption till fasta ytor studerades med hjälp av in situ ellipsometri. Samtidigt mättes aktiviteten av det adsorberade lipaset in situ genom hydrolys av p-nitrofenylacetat. Adsorption mättes till metylerade kiseloxidytor med varierande grad av metylering samt till rent kiseloxid. Olika lipaser studerades; lipas från Humicola lanuginosa (HLL)vildtypen (WT) och enThe adsorption of lipases to solid surfaces was studied by means of in situ ellipsometry. In addition, the activities of the adsorbed lipases were measured in situ, by the hydrolysis of p-nitro phenyl acetate. Adsorption was made to methylated silica surfaces with varying degree of wettability as well as to clean silica surfaces. Lipases from Humicola lanuginosa (HLL) the wild-type (WT) and a m
