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We have made use of a reporter mouse line in which enhanced green fluorescence protein (GFP) is inserted into the Sox1 locus. We show that the GFP reporter is coexpressed with the Sox1 protein as well as with other known markers for neural stem and progenitor cells, and can be used to identify and isolate these cells by fluorescence-activated cell sorting (FACS) from the developing or adult brain
The effect of viruses on the microbial loop, with particular emphasis on bacteria, was investigated over an annual cycle in 2003–2004 in Lake Druzhby and Crooked Lake, two large ultraoligotrophic freshwater lakes in the Vestfold Hills, Eastern Antarctica. Viral abundance ranged from 0.16 to 1.56 × 109 particles L-1;1 and bacterial abundances ranged from 0.10 to 0.24 × 109 cells L-1;1, with the low
The objective of the study was to investigate bone mineral density and bone turnover among female students aged 16-24 years in relation to lifestyle factors, such as dietary habits and physical activity, as well as physiological factors, such as age, body weight, and menstrual pattern. Female college and university students (n = 218) were given a validated questionnaire with 34 questions concernin
Studies, mainly from the UK, show that many farmland birds have declined as a result of recent agricultural intensification. We tested this idea by analysing farmland bird population trends in Sweden, a country displaying less dramatic agricultural changes and less intensive agriculture. Specifically we investigated whether (i) farmland specialists have declined more than generalists, (ii) populat
There is a lack of knowledge to which extent heredity or familial risk factors are involved in the development of chronic bronchitis/emphysema (CBE). Smoking is regarded as the most important risk factor, but only about 15% of smokers develop airway obstruction. We evaluated the importance of familial risk factors compared to smoking and ex-smoking using an epidemiological approach. In 1992, a pos
Active site titration and activity measurements were performed in hexane on lyophilised lipase preparations containing different amounts of phosphate buffer and lipase immobilised on porous polypropylene. Lyophilisation of Thermomyces lanuginosus lipase with large quantities of phosphate salts (200 mm) increased the specific activity fourfold, and the number of rapidly titratable active sites incr
A granulomatous alveolitis, with multinuclear cell formation combined with an eosinophilic peribronchiolitis, was achieved in rats by intratracheal administration of sephadex beads (G-200, Pharmacia, Sweden). The pattern of inflammation and the degree of postgranulomatous fibrosis were substantially dampened when the particles were dispersed by ultrasonification. The animals were analyzed with bro
To examine the possible roles of glutamate and aspartate as neurotransmitters in the nucleus submedius (Sm) of rats, the distributions of these amino acids were examined by electron microscopic immunogold labeling. High levels of glutamate were detected in trigeminothalamic tract terminals anterogradely labeled with horseradish peroxidase conjugates. These terminals also displayed a positive corre
The adsorption of starch that had been hydrophobically modified with octenyl succinate anhydride (OSA) at the oil/water interface during emulsification was studied. The starch samples were of waxy barley origin and were varied in molar mass and degree of substitution (DS). The particle size of the emulsions was measured and the adsorbed amount of starch was determined through serum depletion. The
Mechanical modeling and dynamic mechanical analysis were associated to investigate the viscoelastic properties of an alkyd acrylic hybrid system in connection with its morphology, which was characterized by transmission electron microscopy. Theoretical predictions based on the interlayer model used in direct mode were successfully combined with the experimental viscoelastic data to lead to relevan
We present results of Monte Carlo computer simulations of a coarse-grained hydrophobic-polar G (o) over bar -like heteropolymer model and discuss thermodynamic properties and kinetics of an exemplified heteropolymer, exhibiting two-state folding behavior. It turns out that general, characteristic folding features of realistic proteins with a single free-energy barrier can also be observed in this
We present measurements of collisional broadening coefficients, obtained at atmospheric pressure, by polarization spectroscopy. Using tunable single mode laser radiation at similar to 2 pro, high-resolution infrared polarization spectra were recorded for CO2-Ar and CO2-He binary mixtures. The recorded polarization spectra were fitted with a Lorentzian cubed function form to obtain the broadening c
The rheological properties of wormlike micellar solutions formed by the pseudo-ternary system sodium dodecyl sulfate (SDS)-Al(NO3)(3)-H2O have been studied at a fixed molar ratio Al(NO3)(3):SDS = 5.92:1 as a function of surfactant concentration. The linear data show that solutions behave as a Maxwell fluid and there are two well-defined domains characterized by scaling laws for surfactant concentr
The paper argues that policy-oriented educational research, specifically large-scale quantitative research, has produced a new kind of knowledge, which is herein termed third order knowledge. This expect knowledge distinquishes itself from tacit knowledge, everyday knowledge and social science knowledge through its purposes and sources. Third order knowledge is defined as knowledge of and for expe
Most studies of the relation between psychological performance in the elderly and deficiencies of cobalamin and folate have used methods that determine the blood concentrations of these vitamins, which might not reflect the vitamin status in the tissues. Recently, two new markers, plasma homocysteine and methylmalonic acid, have attracted growing interest since they are considered to reflect the s
Phospholipids are an important constituent of the cell plasma membrane and are also present in most common dietary products, being particularly abundant in milk, egg, meat and beans. Phospholipids are hydrolysed by different phospholipases to generate multiple breakdown products that affect the fate of the cells. Most phospholipids such as phosphatidylcholine, lysophosphatidylcholine, phosphatidyl
This paper examines the admissibility of monetary aggregate groupings for the US over 1993-2001, based upon weak separability. We investigate the impact of retail and commercial demand deposit sweep programs on the separability of monetary asset groupings. Weak separability is tested Using the Swofford-Whitney and Fleissig-Whitney tests. We use Varian's measurement error adjustment procedure to el
Background: The activation peptide released from procarboxypeptidase B, CAPAP, is a marker of the activation of pancreatic enzymes in acute pancreatitis while anionic trypsinogen (AT) levels in urine relate to leakage of unactivated proenzymes. Data on these markers in patients suffering from severe acute abdominal disorders of nonpancreatic origin are lacking. Purpose: To examine levels of CAPAP
