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Nonsense-mediated mRNA decay in barley mutants allows the cloning of mutated genes by a microarray approach.

We have previously described a microarray approach to identify and clone genes from mutants of higher organisms. In the method cDNA of two mutants with similar phenotype are competitively hybridized to DNA clones arrayed on a glass slide. Clones corresponding to an mRNA that is not expressed in one of the strains due to a mutation will be specifically highlighted in the hybridization, which provid

Estimating the Two-Dimensional Coherence Function

In this paper, we extend the one-dimensional Capon-based magnitude square coherence (MSC) spectral estimator, to form two-dimensional Capon- and APES-based MSC spectral estimators. The resulting estimators are found to yield significantly improved estimates as compared to the typical Welch-based estimator. Furthermore, we introduce a computationally efficient time-updating of the presented MSC est

Tracking ultrafast excited-state bond-twisting motion in solution close to the Franck-Condon point

Applying optimal control to photoinduced trans-cis isomerization in condensed phase, the dynamics of bond-twisting motion of 1,1'-diethyl-4,4'-cyanine in methanol and propanol is revealed. The shape of the optimized pulse resulting from minimization of the photoisomer formation can be directly related to the initial excited-state dynamics in close proximity to the Franck-Condon point. The solvent

Color constancy in visual scene perception

In RoboCup, all objects in the game are color coded to allow easy segmentation and identification. However, color vision is very sensitive to the exact color of the illumination which makes the identification task much harder than is often anticipated. Algorithms that work in the lab in fluorescent light may not function at all in an actual game where incandescent lights are used. This article is

Levels of C-14 in the terrestrial environment in the vicinity of two European nuclear power plants

Radiocarbon is produced in all types of nuclear reactors. Most of the C-14 released into the environment is in the form of gaseous emissions. Recent data on the C-14 concentration found in terrestrial samples taken in the vicinity of nuclear power plants in Romania and Lithuania are presented. We found increased C-14 levels in the surroundings of both power plants. At the Romanian power plant Cern

Thermodynamic Modelling of Electrolyte Solutions with Application to the Pulp and Paper Industry

Popular Abstract in Swedish Elektrolyter är ämnen som sönderdelas i ett lösningsmedel och därvid ger upphov till positivt och negativt laddade joner. De starka elektrostatiska krafter i lösningen som detta i sin tur orsakar är typiska för elektrolytlösningar. Redan vid små tillsatser av en elektrolyt till ett lösningsmedel kan lösningens egenskaper förändras kraftigt. Lösningar av elektrolyter speA thermodynamic model for electrolyte solutions has been derived for binary and multicomponent solutions up to very high molalities. The model was extensively tested on 163 binary aqueous electrolyte systems up to very high molalities, and the results show that the model represents mean ionic activity coefficients and osmotic coefficients very accurately. The model parameters vary significantly be

Restricted access chromatographic sample preparation of low mass proteins expressed in human fibroblast cells for proteomics analysis

Two-dimensional electrophoresis and modern image analysis systems have made it possible to study protein expression and regulation of proteins in biological systems. Proteins in the molecular mass region of 20-120 kDa are well investigated and described. However, proteins with masses below 20 kDa are the least investigated as they are rarely seen on 2D-PAGE due to fast migrations in the electric f

The prediction of the nuclear quadrupole splitting of Sn-119 Mossbauer spectroscopy data by scalar relativistic DFT calculations

The electric field gradient components for the tin nucleus of 34 tin compounds of experimentally known structures and Sn-119 Mossbauer spectroscopy parameters were computed at the scalar relativistic density functional theory level of approximation. The theoretical values of the electric field gradient components were used to determine a quantity, V, which is proportional to the nuclear quadrupole

The measurement of alpha(8) from event shapes with the DELPHI detector at the highest LEP energies

Hadronic event shape distributions are determined from data in e(+)e(-) collisions between 183 and 207 GeV. From these the strong coupling alpha(s) is extracted in O(alpha(s)(2)), NLLA and matched O(alpha(s)(2))+ NLLA theory. Hadronisation corrections evaluated with fragmentation model generators as well as an analytical power ansatz are applied. Comparing these measurements to those obtained at a

Work related shoulder disorders: quantitative exposure-response relations with reference to arm posture

Aims: To determine quantitative exposure-response relations between work with highly elevated arms and supraspinatus tendinitis, shoulder pain with disability, and shoulder pain without disability. Methods: A cross sectional study was conducted in a historical cohort of 1886 males from three occupational groups. Exposure measurements were performed for four consecutive working days in a random sam

Transformation of a word model to a geometric space description

On the basis of the Agent-action-Objective (AaO) paradigm, it will be shown how the transformation of a word model to a geometric space description can be made the founda¬tion for an approach to informational invariants. In particular, the word model of a Causal Loop Diagram (CLD) has been used for simplifying the involved procedures. Since the explanation of a causal loop requires a verbal descri

Recursive estimation of parameters in Markov-modulated Poisson processes

A hidden Markov regime is a Markov process that governs the time or space dependent distributions of an observed stochastic process. Recursive algorithms can be used to estimate parameters in mixed distributions governed by a Markov regime. Here we derive a recursive algorithm for estimation of parameters in a Markov-modulated Poisson process also called a Cox point process. By this we mean a doub

Changes in health beliefs after labelling with hypercholesterolaemia.

BACKGROUND: Patients' health beliefs influence their willingness to comply with medical advice. In an earlier study, it was found that men with a previous history of information on risk factors for ischaemic heart disease expressed more feelings of threat to their health than did men without this experience. As anxiety may have adverse effects, such as making patients avoid the desired action, thi

Arrhythmia as a result of poor intercellular coupling in the sinus node: A simulation study

The effects of reduced intercellular coupling in the sinus node were investigated by means of simulations. Coupling was reduced both uniformly, and by introducing localized interaction blocks. In either case, model sinus node element networks typically splitted into frequency domains. These were defined as groups of neighbour elements which all attained the same mean firing frequency. In systems,