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Histone chaperones control nucleosome density and chromatin structure. In yeast, the H3-H4 chaperone Spt2 controls histone deposition at active genes but its roles in metazoan chromatin structure and organismal physiology are not known. Here we identify the Caenorhabditis elegans ortholog of SPT2 (CeSPT-2) and show that its ability to bind histones H3-H4 is important for germline development and t
Genotoxic DNA double-strand breaks (DSBs) can be repaired by error-free homologous recombination (HR) or mutagenic non-homologous end-joining 1 . HR supresses tumorigenesis 1 , but is restricted to the S and G2 phases of the cell cycle when a sister chromatid is present 2 . Breast cancer type 1 susceptibility protein (BRCA1) promotes HR by antagonizing the anti-resection factor TP53-binding prote
During DNA replication, chromatin is reassembled by recycling of modified old histones and deposition of new ones. How histone dynamics integrates with DNA replication to maintain genome and epigenome information remains unclear. Here, we reveal how human MCM2, part of the replicative helicase, chaperones histones H3-H4. Our first structure shows an H3-H4 tetramer bound by two MCM2 histone-binding
To evaluate the behaviour of a single timber-concrete composite (TCC) T-beam, a simplified 2D finite element (FE) model in Abaqus is developed and validated using experimental tests and the Gamma method given in Annex B of EN 1995–1-1. The shear connection used in the beam consist of perfobond connectors, which are adhesively bonded to the timber beam. In the developed model, beam and shell finite
In timber-concrete composites (TCC), more researchers are trying to find the “ultimate” shear connector, which combines multiple advantages. Bonded steel plates are one of the possible solutions. This paper provides an analytical and a numerical approach, calibrated with previously conducted experiments, to predict the load carrying capacity, and corresponding governing failure mode, of perfobond
With the need of renovation in the current timber structures, use of a cement based layer is a popular solution, which improves the structural integrity and increases the structural mass. In this paper, the use of a lightweight concrete with dowel type connections is investigated since such a combination can serve for both new and existing structures. Two types of dowel connections are experimenta
In this paper, perforated shear connectors (perfobond), used extensively in steel-concrete composite (SCC) beams, are implemented in timber-concrete composite beams (TCC) and experimentally investigated. In contrast to its original use, the perfobond connector is connected with an adhesive to the timber beam while welded in the SCC solution. On the concrete slab side, connection is achieved using
In this paper, the use of low strength lightweight concrete in combination with glue-laminated timber (TLCC) is studied. Six series of push-out tests were conducted to assess the mechanical performance of the screw shear connection between these materials with a perpendicular and inclined (shearcompression)configuration with various screw diameters and lengths. The tests show that though low stren
Produktion av dricksvatten är en fundamental del av vårt samhälles infrastruktur och ökad förståelse för de faktorer som avgör dricksvattenkvaliteten är därmed av stort värde. En av dessa faktorer är mikrobiomen, alltså vilka mikroorganismer som lever i vattnet. Dessa kommer huvudsakligen från så kallad biofilm, ett tunt slem-liknande skikt som formas på insidan av vattenrör. Skiktet skapas av ämnThe development of the water-borne microbiome in newly installed polyethylene pipes of a non-chlorinated drinking water distribution system was studied over a period of six months. Water samples were taken from two identical newly installed pipes (“NP1”, “NP2”), as well as from a reference point at equal distance (“DS1”). These water samples were filtered and had the DNA extracted using FastDNA SP
Invasive species can cause serious damage to ecosystems and built-up structures. “Japanese knotweed” is one of the most problematic invasive plant species in Scania. It has spread in a fast and uncontrollable manner throughout the years, and major efforts are being made to decrease its extension and further spread. Once established, it's difficult to exterminate without causing environmental h
After DNA replication, chromosomal processes including DNA repair and transcription take place in the context of sister chromatids. While cell cycle regulation can guide these processes globally, mechanisms to distinguish pre- and post-replicative states locally remain unknown. Here we reveal that new histones incorporated during DNA replication provide a signature of postreplicative chromatin, re
Användningen av artificiell intelligens ökar inom många områden i det samtida samhället, inklusive datorsynssystem som bygger på maskininlärning och artificiella neuronnätverk. Dessa system omformar handlingsmöjligheter och beskrivs ofta som ”svarta lådor” som utmanar etablerade former av vetenskaplig förståelse och teknisk kontroll. Det är därför viktigt att förstå de aktiviteter genom vilka sådaThe uses of artificial intelligence are increasing across many areas of contemporary society, including computer vision systems involving machine learning and artificial neural networks. These systems rearrange action possibilities and are often described as “black boxes” that challenge conventional modes of scientific understanding and engineering control. It is important to understand the activi
Small bodies exist in distinct populations within their planetary systems. These reservoir populations hold a range of compositions, which to first order are dependent on formation location relative to their star. We provide a general overview of the nature of the reservoirs that source exocomets, from the influence of the stellar environment through planetesimal formation to comparisons with Sola
Malaria remains a great public health concern, with an estimated 263 million cases and 597,000 estimated deaths in 2023. Artemisinin-based combination therapies (ACTs) are currently the first-line treatment for uncomplicated P. falciparum malaria in Sub-Saharan Africa (SSA), but the emergence of resistant parasites is threatening their efficacy. Single-nucleotide polymorphisms (SNPs) in the pfk13
Parkinson’s disease (PD) is a progressive neurodegenerative disease, characterized by degeneration of dopaminergic neurons, leading to notable difficulties with one’s movement. Among different treatment options, stem cell replacement therapies have been recognized as an option to not only treat disease symptoms but to address its underlying cause- neuronal degeneration. This can be achieved throug
Kan kreatinmetabolismen vara ett potentiellt behandlingsmål för lungcancer i skivepitel? Lungcancer är en av världens vanligaste typer av cancer. Varje år rapporteras över 2 miljoner nya lungcancer fall och 1,8 miljoner dödsfall i världen enligt statistik från 2022. Cancer uppstår som en konsekvens av både arv och miljö. Den vanligaste orsaken till lungcancer är rökning. De två vanligaste typerLung cancer is one of the most common cancer types and the leading cause of cancer-related mortality. Squamous cell carcinoma (SqCC) of the lung is the second most common histological subtype of lung cancer and the most prevalent in smokers. It is aggressive and hard to treat due to a lack of identified driver mutations and unresponsiveness to treatment. Finding new targeted therapies for SqCC is
Background Venture Creation Programs (VCPs) provide a unique experiential learning setting in which real-world uncertainty, emotional dynamics, and iterative learning cycles shape entrepreneurial decision-making. Research Gap While prior research has established the importance of effectuation and causation in entrepreneurship, less is known about the factors influencing students’ use of these log
The call is coming from inside: your body’s own defences might be against you Mycobacterium avium is a species of bacteria, closely related to tuberculosis, that can cause pulmonary disease in people. Despite this, we likely come in contact with these microorganisms every day. They can be found in water reservoirs and in the soil, entering the lungs of the host – like humans and rodents – through
Why studying proteins is important All our cells contain hereditary information, DNA, that allows the building blocks of our body, the cells, to know how to behave. However, the DNA is only the instruction book while proteins are the key players doing all the important work. We have to cope with many situations throughout our lives, such as stress, hunger and sickness among various others, and it
