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In microbiology, cell analysis is an important tool for understanding influences on biotechnological production. Using current bulk methods, the secretome cannot be assigned to the corresponding cell. This contribution reports a novel method for cell separation inside microscale droplets. Cells are focused in droplets using bulk acoustic waves before the droplet is split at a bifurcation. For a wi

This work presents a novel concept for acoustofluidic cell manipulation: thin vibrating channel walls. Improved etch recipes enable us to produce thin walls between two microfluidic channels, fabricated in silicon or via PDMS casting, that can be used for challenging microbiological applications. We demonstrate the medium exchange for bacteria utilised for transformation, single-cell rotation for

This work presents an acoustofluidic chip capable of separating cells form their corresponding secretome for subsequent MALDI-MS analysis, which can be used amongst many other applications for directed evolution.

This contribution reports a novel method for cell-supernatant separation inside nanoliter droplets with separation efficiencies for single cells of 100% at flow speeds of 2 mm/s. Cells are focused in nanoliter droplets using bulk acoustic waves before the droplet is split at a bifurcation into a supernatant- and a cell-containing droplet. The method enables upconcentration of cells, cell washing,

Model Hamiltonians are regularly derived from first principles to describe correlated matter. However, the standard methods for this contain a number of largely unexplored approximations. For a strongly correlated impurity model system, here we carefully compare a standard downfolding technique with the best possible ground-truth estimates for charge-neutral excited-state energies and wave functio

Background: Low grip strength and gait speed are associated with mortality. However, investigation of the additional mortality risk explained by these measures, over and above other factors, is limited. Aim: We examined whether grip strength and gait speed improve discriminative capacity for mortality over and above more readily obtainable clinical risk factors. Methods: Participants from the Heal

Background: The Ponseti method for treating clubfoot consists of initial treatment with serial casting accompanied by achillotenotomy if needed, followed by the maintenance phase including treatment with a foot abduction orthosis (FAO) for at least four years. This study aimed to examine the duration, course, and outcome of orthotic treatment in children with clubfoot. Methods: 321 children with c

The toxicity of particulate matter (PM) is dependent on particle physical and chemical properties and is commonly studied using in vivo and in vitro approaches. PM to be used for in vivo and in vitro studies is often collected on filters and then extracted from the filter surface using a solvent. During extraction and further PM sample handling, particle properties change, but this is often neglec

During the 1980s, Sweden faced a substantial intelligence threat from Soviet and Warsaw Pact attempts at collection and infiltration. Despite this, Swedish counterintelligence managed to launch several effective responses, including early detection of recruitment attempts and expulsion of a number of hostile intelligence officers operating under diplomatic cover. One part of this was the willing c

Viljan att skydda växter och djur får ibland orimliga effekter. Det skriver flera företrädare för Moderaternas arbetsgrupp för en kommande miljöpolitik på DN debatt.Farlig polariseringDe tar bland annat upp viktiga frågor om ”människans plats i naturen”. Men konflikten mellan utveckling och miljö är en återkommande missuppfattning. Frågan om naturvården främst bör utgå från människans eller nature

This paper investigates social workers' perceptions of their relationships with clients across three Swedish different municipalities. By selecting three municipalities at varying stages of digitalization, the study facilitates a comparison of social workers' accounts. The comparison highlights differences in the descriptions of the relationship between social workers and clients, shedding light oThis paper investigates social workers' perceptions of their relationships with clients across three Swedish different municipalities. By selecting three municipalities at varying stages of digitalization, the study highlights differences in the descriptions of the relationship between social workers and clients, shedding light on whether, and if so, how, digital tools influence this relationship.

Open office environments are architectural solutions that are believed to economize the use of office space and facilitate social interactions at work. However, research has shown that employees in open office environments may experience lower job satisfaction, stress reactions, and exhibit poorer performance. Exposure to noise in open office environments may prompt avoidance behaviours such as wo

Individuals may vary in their sensitivity to noise and this may affect how they relate to their surroundings. As part of an intervention project about the psychosocial effects of informational sound masking, we examined the occurrence of self-labeled noise sensitive individuals as well as the association between three indicators of noise sensitivity and one indicator for the perceived enjoyment of

This intervention seeks to revivify democratic thinking in political geography, through foregrounding and pluralising its material and temporal dimensions. At the same time, it speaks to a renewed centrality and relevance of infrastructure and infrastructural projects in political discourse. The contributions included here demonstrate how an infrastructural lens can offer new insights into democra

The ubiquity of microplastics ecosystems has gained global attention. In this context, Latin America, which is responsible for 8% of the world's consumption of plastic material and has a small recycling rate (4.5%), does not have enough data on microplastics contamination in its environmental matrices. This systematic review analyzed 196 studies from 16 territorials in Latin America and provided i

Microplastic (MP) contamination is a topic of growing global concern; these particles are ubiquitous in environmental ecosystems and have been found in aquatic, terrestrial, and atmospheric mediums. However, the protocols to quantify MPs in environmental samples have limitations and may lead to overestimation and/or underestimation of the plastic debris. Therefore, the aim of this research was to

Direct photolysis of the emerging contaminant 2-(thiocyanomethylthio) benzothiazole (TMCTB) was performed in aqueous solution at different concentrations with high-pressure mercury lamp (5.0, 8.0, 13.0, 16.0, 20.0, 23.0, 27.0, 35.0, 40.0, 45.0, and 50.0 mg L− 1) and with natural sunlight radiation (6.0, 30.0, and 60.0 mg L− 1). TCMTB underwent rapid degradation by direct photolysis with a high-pre

The ubiquitous presence of microplastics in the aquatic environment has raised concern about their potential impacts on and risks to the biota. While the presence of microplastics in a marine environment has been well studied, the impact of microplastic contamination in freshwater bodies is understudied. In the present study, baseline data about contamination with microplastics in Lake Guaíba in s