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Fibromodulin belongs to the small leucine-rich repeat proteoglycan family, interacts with collagen type I, and controls collagen fibrillogenesis and assembly. Here, we show that a major fibromodulin-binding site for collagen type I is located in leucine-rich repeat 11 in the C terminus of the leucine-rich repeat domain. We identified Glu-353 and Lys-355 in repeat 11 as essential for binding, and t
The purpose of this work was to study how the pixel size of digital detectors can affect shape determination of microcalcifications in mammography. Screen-film mammograms containing microcalcifications clinically proven to be indicative of malignancy were digitised at 100 lines/mm using a high-resolution Tango drum scanner. Forty microcalcifications were selected to cover an appropriate range of s
The composition and mode of attachment of Cu(II) complexes at the gamma-Al2O3-water interface in suspensions containing a simple amino acid (glutamate) were characterized with EXAFS and FTIR spectroscopies. The spectroscopic results indicate that two types of Cu(II)-glutamate-alumina interactions are primarily responsible for Cu(II) and glutamate uptake between pH 4 and 9. In acidic suspensions of
Experimental and theoretical uncertainties in the measurement of cloud condensation nuclei (CCN) with a continuous-flow thermal-gradient CCN counter from Droplet Measurement Technologies (DMT-CCNC) have been assessed by model calculations and calibration experiments with ammonium sulfate and sodium chloride aerosol particles in the diameter range of 20–220 nm. Experiments have been performed in th
We studied a vertical 'quantum dot molecule', where one of the dots is occupied by electrons and the other by holes. We find that different phases occur in the ground state, depending on the carrier density and the interdot distance. When the system is dominated by shell structure, orbital degeneracies can be removed either by Hund's rule, or by Jahn-Teller deformation. Both mechanisms can lead to
Two conformationally different [1,2,7,8]tetrathiacyclododecino[4,3-b:5,6-b':10,9-b":11,12-b''']tetraind oles 9a and 9b have been isolated in good yields, and the existence of a third conformer 9c in solution was demonstrated by mass spectrometry and H-1 NMR spectroscopy. The interconversions of the tetraindoles 9a-c have also been studied. The conformation of 9b was confirmed by X-ray crystallogra
The hypothesized existence of a Neoproterozoic supercontinent called Rodinia is based on a series of geological and paleomagnetic observations, with details about the configuration and evolution of this supercontinent still a matter for debate. Regrettably, we found that the comment by Piper (this volume) lacks scientific objectiveness. The 'Palaeopangaea' that he promotes is, in our view, based o
The treatment of simulated wastewater containing Acid Scarlet BS was tested with a two-step process that the pretreatment of Fe(II)-mediated decolorization was followed by the electrochemical oxidation with a three-phase three-dimensional electrodes. The experimental results showed that the decolorization mechanism was reductive destruction for the azo group of the dye molecular rather than floccu
This study was aimed at finding a correlation between the experienced off-flavor in packed foods and the presence of specific degradation products in LDPE packaging films. The possibility to trap degradation products by chemical reactions with scavengers, i.e., a zeolite additive or antioxidants, was investigated This would prevent degradation products from migrating to the polymer film surface an
Aims/hypothesis The mechanisms by which glucose regulates glucagon release are poorly understood. The present study aimed to clarify the direct effects of glucose on the glucagon-releasing alpha cells and those effects mediated by paracrine islet factors. Materials and methods Glucagon, insulin and somatostatin release were measured from incubated mouse pancreatic islets and the cytoplasmic Ca2+ c
Microdialysis was used to study how ischemia-evoked gastric mucosal injury affects rat stomach histamine, which resides in enterochromaffin-like (ECL) cells and mast cells. A microdialysis probe was inserted into the gastric submucosa, and the celiac artery was clamped (30 min), followed by removal of the clamp. Microdialysate histamine was determined by enzyme-linked immunosorbent assay. In addit
Malignant fibrous histiocytoma (MFH) represents a heterogeneous soft tissue sarcoma entity. The authors compared different methods to determine immunohistochemical staining in whole tissue sections, evaluated the tissue microarray technique, and assessed immunohistochemical heterogeneity using the proliferation marker Ki-67 in 47 histopathologic tumor blocks from 11 MFHs. Whole tissue sections wer
Rotational Raman linewidths calculated from three different models have been used in temperature measurements by rotational coherent anti-Stokes Raman scattering (CARS)—a semiclassical ab initio model, the modified exponential energy gap model (MEG), and the energy corrected sudden scaling law (ECS). Experimental rotational CARS spectra were generated, using the dual-broadband approach, in pure ni
The fermentability and pattern of short-chain fatty acids (SCFA) formed in the hindgut of rats given various combinations of dietary fibers (DF) and resistant starch (RS) were investigated. Highly fermentable indigestible carbohydrates, i.e., guar gum (GG), pectin (Pec) and high amylose cornstarch (HAS), and a DF with a relatively high resistance to fermentation, i.e., wheat bran (WB), were includ
Background and aims: Islet function is regulated by islet autonomic nerves. These nerves harbour not only the classical neurotransmitters, acetyl choline and noradrenaline, but also neuropeptides. This study examined whether the neuropeptides, pituitary adenylate cyclase activating polypeptide (PACAP) and gastrin releasing polypeptide (GRP) contribute to the regulation of insulin secretion in mode
