In basic and applied biotechnology, design of affinity ligands has become essential for high-capacity applications such as affinity-based downstream processes for therapeutic molecules. Here, we established a proof-of-concept for the use of multimeric fusion single-chain variable fragment (scFvs) as high-capacity ligands in affinity adsorbents. Mono- and di/tri-scFvs separated by Pro-rich negative

Amyloid fibrils of α-synuclein (α-syn) are a component of Lewy bodies, the characteristic hallmark of Parkinson's disease. Amyloid fibrils arise through primary nucleation from monomers, which in the case of α-syn is often heterogeneous, followed by the growth of the nuclei by monomer addition. Secondary nucleation corresponds to the formation of new fibrils facilitated by pre-existing fibrils. Wh

Objective: To achieve the full potential of sentinel lymph node (SLN) detection in endometrial cancer, both presumed low- A nd high-risk groups should be included. Perioperative resource use and complications should be minimized. Knowledge on distribution and common anatomical sites for metastatic SLNs may contribute to optimizing the concept while maintaining sensitivity. Proceeding from previous

We explore the dynamics of income and income inequality under asymmetric information in credit markets. Within a stochastic overlapping-generations framework, we study the investment decision of entrepreneurs with heterogeneous abilities. Under information asymmetry, banks do not observe entrepreneurial ability and offer a single pooled loan contract to all entrepreneurs. We show that, following a

Aim: Specific birth-related fractures have been studied; underestimates might be a problem. We aimed to assess all fractures diagnosed as birth-related as well as other neonatal fractures. Methods: A population-based study on all infants born in Sweden 1997-2014; data were retrieved from the Swedish Health Registers (10th version of International Classification of Diseases. Outcome measures were b

Objective. Evaluate if the use of active clearance of chest tubes after aortic valve surgery influenced bleeding and reduced postoperative residual pericardial effusion. Design. Prospective randomised trial comparing PleuraFlow® 32 F chest tube with FlowGlide™ active clearance to a standard Argyle® 32 F chest tube in 100 patients undergoing aortic valve surgery. Chest tube outputs and pericardial

The strategic direction of the European Union (EU) over the past twenty years has focused on increasing resource productivity and innovation in the economy, aiming at the efficient use and secured supplies of resources, economic growth and job creation, with fewer environmental impacts. One of the basic premises of the new ‘Green Deal’ for Europe is the promotion of the circular economy. The aim o

The STRIPAK complex has been linked to a variety of biological processes taking place during embryogenesis and development, but its role in cancer has only just started to be defined. Here, we expand on previous work indicating a role for the scaffolding protein STRIP1 in cancer cell migration and metastasis. We show that cell cycle arrest and decreased proliferation are seen upon loss of STRIP1 i

Background: Symptoms of depression and anxiety are common in children with obsessive-compulsive disorder (OCD) and associated with more severe OCD, greater impairment, and worse treatment outcome. Beyond twin studies showing that genetic factors contribute to the high co-occurrence, few studies have examined how OCD, depression, and anxiety are linked in youth, and current studies often fail to ac

Much has been written about Percy Bysshe Shelley’s engagement with different aspects of natural philosophy in his poetry and prose. But Shelley’s interest in plants and botany has yet to receive sustained attention. This chapter focusses on the representation of wildness in what it defines as Shelley’s botanical poetry: poems which not only have a plant as their ostensible subject, or which develo

According to the waste hierarchy principle, which constitutes the basis of European waste legislation, waste prevention and re-use are considered – most of the times – better waste management options than recycling. However, prevention and re-use activities are difficult to operationalise and measure, without a monitoring framework in place. This contribution investigates the potential of re-using

Neuroscience focusing on human development and disease has long been hampered due to ethical reasons, low tissue availability, and low translatability from animal models. To circumvent these obstacles, we have developed two methods for the investigation of human neural cells in culture. Firstly, we present a robust 2-week protocol for the differentiation of human pluripotent stem cells (PSCs) into

The photochemical reaction pathways of CHBr3 in solution were unveiled using two complementary X-ray techniques, time-resolved X-ray solution scattering (TRXSS) and X-ray transient absorption spectroscopy, in a wide temporal range from 100 ps to tens of microseconds. By performing comparative measurements in protic (methanol) and aprotic (methylcyclohexane) solvents, we found that the reaction pat

Economic theory predicts that differences in wages for workers with similar skills will decline as labour mobility increase. This prediction is reminiscent of the ‘law of one price’, the notion that markets, if unfettered, eliminates price differentials of similar commodities across space. But can we really apply the economic logic of the commodity market to the labour market, governed as it is by

The sharing economy has become prominent in the policy, business and academic discourse as a way to achieve greater levels of resource efficiency in sustainability transitions. An example is carsharing, which is regarded as a potential lever for change in sustainable mobility transitions. In this article we, apply the "ecologies of business models" perspective to investigate how carsharing initiat

Fear has been assigned a central role in models of obsessive–compulsive disorder (OCD), but empirical investigations into the emotions that underpin OCD symptoms are few, especially in pediatric samples. Using validated, clinician-led structured interviews, 124 youth with OCD reported on the presence and severity of symptoms across the main symptom dimensions of OCD (aggressive, symmetry, contamin

Refugees have moved into the spotlight of public debate in Europe and North America, where they are targeted by multiple welfare state interventions. This volume analyses the tensions that emerge within the strong welfare states of Northern Europe when faced with an increased immigration of protection-seeking people. Examining the encounter between refugees and the welfare states, this book explor

Refugees have moved into the spotlight of public debate in Europe and North America, where they are targeted by multiple welfare state interventions. This volume analyses the tensions that emerge within the strong welfare states of Northern Europe when faced with an increased immigration of protection-seeking people. Examining the encounter between refugees and the welfare states, this book explor