Pre-PCR Processing Strategies for Quantitative Detection of Food-Borne Pathogens using Real-Time PCR
Since the development of real-time PCR, the technology has been widely applied in the field of diagnostics. In comparison with conventional PCR it has opened up the possibility of accurate quantification of microorganisms in clinical, environmental and food samples. When employing real-time PCR and absolute quantification in biological samples, five important requirements should be fulfilled, name
