A novel approach in lactose based induction for enhanced production of 1-4-beta xylanase by recombinant Escherichia coli
Escherichia coli BL21 (DE3) with a plasmid vector pET-22b (+) carrying xylanase coding gene isolated from an extremely thermophilic bacterium, Thermotoga sp. was used in this work for enhanced production of recombinant xylanase. The study was focused on optimization of lactose based induction for enhanced production of xylanase. Data collected from OFAT and DoE based trails indicated that initiati