Lipases at Solid Surfaces An Adsorption and Activity Study
The adsorption of lipases to solid surfaces was studied by means of in situ ellipsometry. In addition, the activities of the adsorbed lipases were measured in situ, by the hydrolysis of p-nitro phenyl acetate. Adsorption was made to methylated silica surfaces with varying degree of wettability as well as to clean silica surfaces. Lipases from Humicola lanuginosa (HLL) the wild-type (WT) and a m