Filtyp
Telocytes (TCs) are recently described interstitial cells, present in almost all human organs. Among many other functions, TCs regulate gastrointestinal motility together with the interstitial cells of Cajal (ICCs). TCs and ICCs have close localization in the human myenteric plexus; however, the exact spatial relationship cannot be clearly examined by previously applied double immunofluorescence/c
Professional antigen-presenting cells, such as dendritic cells, macrophages and B cells have been implicated in the pathogenesis of rheumatoid arthritis, constituting a possible target for antigen-specific immunotherapy. We addressed the possibility of blocking antigen presentation of the type II collagen (CII)-derived immunodominant arthritogenic epitope CII259-273 to specific CD4 T cells by inhi
OBJECTIVE: Periodontal ligament (PDL) cells express oestrogen receptor beta (ERbeta) protein, but cellular functions regulated by ERbeta in these cells have not been identified. In this study we determine if ERbeta is localised to mitochondria and if oestrogen regulates mitochondrial function in human PDL cells obtained from teeth extracted for orthodontic reasons. DESIGN: Subcellular distribution
Amyloid β peptide (Aβ) is the crucial protein component of extracellular plaques in Alzheimer’s disease. The plaques also contain gangliosides lipids, which are abundant in membranes of neuronal cells and in cell-derived vesicles and exosomes. When present at concentrations above its critical micelle concentration (cmc), gangliosides can occur as mixed micelles. Here, we study the coassembly of th
Dye affinity chromatography is one of the chromatography techniques which uses dye as a ligand for protein purification. In this study, single-layer poly(acrylamide-allyl glycidyl ether) cryogel was prepared, which contains free epoxy groups at sub-zero temperature. The double-layer was prepared inside a primary single-layer cryogel to form a secondary network inside the gel. Cibacron Blue F3GA wa
Antibodies to double-stranded (dsDNA) are associated with systemic lupus erythematosus (SLE) and directly involved in human lupus nephritis. Information about their glomerular target antigens is inconsistent, and whether availability of target antigens, antibody specificity or avidity are nephritogenic parameters, is not determined. In this study, we analyzed renal tissue from anti-dsDNA antibody-
Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by an impaired epidermal barrier, dysregulation of innate and adaptive immunity, and a high susceptibility to bacterial colonization and infection. In the present study, bacterial biofilm was visualized by electron microscopy at the surface of AD skin. Correspondingly, Staphylococcus aureus (S. aureus) isolates from lesion
Poly(d,l-lactide-co-glycolide) (PLG) is the most frequently used biodegradable polymer in the controlled release of an encapsulated drug. The purpose of this work was to explain the surprisingly slow diffusion through this polymer, and locate the major source of mass transport resistance. Diffusion of human growth hormone (hGH) and glucose through PLG films was undetectable (using a diffusion cell
Cooperative binding is a key feature of metabolic pathways, signaling, and transport processes. It provides tight regulation over a narrow concentration interval of a ligand, thus enabling switching to be triggered by small concentration variations. The data presented in this work reveal strong positive cooperativity of α-synuclein binding to phospholipid membranes. Fluorescence cross-correlation
Transfer of melanin-containing melanosomes from melanocytes to neighboring keratinocytes results in skin pigmentation. To provide a more practical method of visualizing melanosomes in melanocytes as well as in keratinocytes, we attempted to use murine cell lines instead of human primary cells. We generated various fluorescent fusion proteins of tyrosinase, a melanin synthesis enzyme located in the
Lipid nanoparticles (LNPs) have emerged as an efficient platform for mRNA delivery, in which ionisable lipids play a central role in intracellular uptake and endosomal escape. In this study, the influence of different ionisable lipids on cellular uptake, endosomal damages and mRNA release kinetics was investigated to identify promising LNP compositions for effective mRNA delivery. Dose-dependent L
250224 Name of PI Malin Parmar Faculty/Department Faculty of medicine/Experimental medical science (EMV) E-mail malin.parmar@med.lu.se Telephone +46 46 222 06 20 Cost center 314062 Responsible researcher Edoardo Sozzi E-mail to researcher edoardo.sozzi@med.lu.se Project title Fabrication of microfluidic devices for connectoid formation Aim of project (max 1100 characters) The aim of this first exp
https://www.bme.lth.se/sites/bme.lth.se/files/2025-10/12_MalinParmar_250224.pdf - 2026-10-06
Biological membranes and human interfaces are maintained under non-equilibrium conditions, where they are constantly exposed to different stimuli. The aim of this work is to advance our knowledge of human interfaces and evaluate how they respond to stimuli they naturally encounter, such as fluctuations in external humidity conditions, temperature, or UVB irradiation. In the first part of this thes
