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Graft polymerization of glycidyl methacrylate onto the pore surface of polyacrylamide macroporous gel was implemented in DMSO-aqueous solution using diperiodatocuprate(III) complexes as an initiator. The grafting densities up to 410% were achieved. The graft polymerization was confirmed by gravimetrical methods and FTIR. The graft polymerization of polymer inside the pores of the macroporous gel r

RNA architectonics offers the possibility to design and assemble RNA into specific shapes, such as nanoscale 3D solids or nanogrids. Combining the minute size of these programmable shapes with precise positioning on a surface further enhances their potential as building blocks in nanotechnology and nanomedicine. Here we describe a bottom-up approach to direct the arrangement of nucleic acid nanost

Background and aims: TCF7L2 is both an activator and an inhibitor of transcription and the most highly associated type 2 diabetes gene known to date. It influences beta cell survival and function, i.e. incretin hormonal effects, insulin processing and secretion. However, its target genes in pancreatic islets are not fully described and the molecular mechanism whereby it propagates its effects on i

Relative Energy Deficiency in Sport (RED-S) is a condition arising when dietary energy intake is insufficient to meet the combined demands of training and normal physiological functions. RED-S negatively impacts bone health, and increases the risk of stress fractures. Yet, its effects on bone matrix organization and overall bone quality is not well understood. Collagen and the osteocyte cells'

Whey protein powder functionality is expected to be closely linked to both structure and properties of the proteins. It is essential that whey protein powders are easily dispersed and dissolved in order to fulfil the specified nutrient content and the functionality in the final product. Poor rehydration can cause challenges on an industrial level as well as for the consumers. In this thesis, severWhey protein powder functionality is expected to be closely linked to both structure and properties of the proteins. It is essential that whey protein powders are easily dispersed and dissolved in order to fulfil the specified nutrient content and the functionality in the final product. Poor rehydration can cause challenges on an industrial level as well as for the consumers. In this thesis, sever

Background: Monoclonal antibodies (mAb) are important tools in the management of tumor disease, and the discovery of antibodies with both specific cancer cell targeting and capacity to enter the cells by internalization are critical to improve the therapeutic efficacy. Method: Antibody cancer cell targeting and internalization properties of fluoroscein-conjugated mAb made against Lewis Y (BR96) we

Mixed cultures of different EPS-producing Streptococcus thermophilus strains in combination with a Lactobacillus delbrueckii subsp. bulgaricus strain with negligible EPS-production were used for yoghurt production. The yoghurt texture was characterised with respect to sensory, rheological and microstructural properties and the EPS-concentrations were determined. The cultures resulted in yoghurts w

The folate metabolism enzyme MTHFD2 (methylenetetrahydrofolate dehydrogenase/cyclohydrolase) is consistently overexpressed in cancer but its roles are not fully characterized, and current candidate inhibitors have limited potency for clinical development. In the present study, we demonstrate a role for MTHFD2 in DNA replication and genomic stability in cancer cells, and perform a drug screen to id

Inom fysiken används ofta hårda sfärer som modellsystem för att beskriva beteenden och strukturer hos grundläggande byggstenar såsom atomer och molekyler. Dessa sfärer kan ses som en samling biljardbollar och kan därav bara organiseras på ett begränsat antal sätt. Med den ökade förståelsen för många komplexa fysikaliska fenomen och den snabbt växande utvecklingen av nya material behövs modellsysteSoft and deformable ionic microgels such as poly(N-isopropylacrylamide-co-acrylic acid), PNIPAM-co-AA, microgels have shown to be a versatile alternative to the already well-established hard sphere model systems. Their tuneable interaction potential makes them well suited as model systems to study the phase behaviour found for particles interacting via a soft isotropic potential. This stems from t

The human gastrointestinal tract (GIT) is home to a large community of microorganisms that contribute to human health. Delivering live bacteria to the gut for therapeutic purposes can thus be highly beneficial. However, delivering live biotherapeutic products or probiotic bacteria to the GIT presents challenges because the cells must remain viable during production, storage, and administration. En

In atherogenesis, free oxygen radicals cause a lipid peroxidation of apoB100-containing lipoproteins in the blood, at the blood–endothelium-interface and in the subendothelial space. These lipoproteins easily aggregate, bind to their receptor heparan sulfate proteoglycan and calcify to arteriosclerotic nanoplaques (ternary complexes). Nanoplaque formation was measured by ellipsometry, both in vitr

Recently a new field of microscopy has emerged, known as selective plane illumination microscopy (SPIM). Using a SPIM setup the sample is illuminated with a thin light sheet from the side. The selective plane illumination overcomes many problems that conventional microscopes have. Conventional microscopes illuminate the sample axially and collects the light only from the focal plane of the microsc

A spectrometer has been developed for use with a confocal scanning laser microscope. With this unit, spectral information from a single point or a user-defined region within the microscope specimen can be recorded. A glass prism is used to disperse the spectral components of the recorded light over a linear CCD photodiode array with 256 elements. A regulated cooling unit keeps the detector at 277