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Objective: To evaluate the barrier properties of intestinal mucosa chronically exposed to urine and to evaluate possible differences between ileal and colonic segments used in the reconstruction of the urinary tract. Materials and methods: Mucosal specimens from patients with continent reservoirs with an abdominal stoma, or orthotopic neobladders constructed from colonic segments, were obtained at
1. 1. Endogenous phospholipase A2 (PLA2) inhibitors in human plasma and in plasma from the hedgehog (Erinaceus europaeus) were demonstrated. 2. 2. The PLA2 activity increased 45-fold in human plasma when a PLA2 binding factor was removed. Furthermore, two peaks of PLA2 inhibitory activity were found after DEAE-chromatography. 3. 3. High levels of PLA2 inhibitory activity was found in plasma from t
Substantial progress has been made in understanding the rate, the pathways, and the mechanisms regulating alveolar protein removal from the uninjured lung. Whole animal studies and cellular studies have demonstrated that the majority of alveolar epithelial protein clearance occurs by passive nondegradative diffusional pathways. Some evidence, however, has been recently presented that alveolar epit
The possible role of CORTISOL in development of the exocrine pancreas was investigated. In the first study, fetal pigs were removed from the uterus by caesarean section (pentobarbitone anesthesia) and the pancreas collected from 51 69 to 109-day-old fetuses (term = 114 ± 2 days). Of these, 15 88 to 90-day-old fetuses had been infused subcutaneously for 6 days with either saline, adrenocorticotropi
A comparative study of antibacterial activity of pancreatic juice was conduced on six mammalian species. Pancreatic juice collections were conducted as acute (rabbit, guinea pig, rat) and chronic (pig, sheep, cattle) experiments, in the former before and after stimulation [cholecystokinin (CCK) and secretin] and in the latter under basal conditions alone. Antibacterial activity was tested on Micro
Due to the increasing share of older people worldwide, the United Nations has directed an initiative towards a "decade of healthy ageing" to promote "age-friendly environments". This study aims to gain a deeper understanding of how to promote social participation and everyday mobility in later life. The ambition is to increase the knowledge about how to create more age-friendly environments and soDue to the increasing share of older people worldwide, the United Nations has directed an initiative towards a "decade of healthy ageing" to promote "age-friendly environments". This study aims to gain a deeper understanding of how to promote social participation and everyday mobility in later life. The ambition is to increase the knowledge about how to create more age-friendly environments and so
The regional small intestinal permeability to the vasopressin analogue desmopressin (dDAVP) was further characterized in proximal jejunal and distal (ileocecal) segments of the rat. Administration of the peptide to closed small intestinal loops confirmed the existence of regional absorption differences also in vivo in rats. Thus, the extent of absorption was about 4 times as high from the ileoceca
Abstract: The biliary excretion of the vasopressin analogue 1‐deamino‐8‐D‐arginine vasopressin (dDAVP) was determined in the pig after three administration routes, intrajugular venous, intraportal venous and intraduodenal. In all cases the biliary excretion was less than 1% of the administered dose. The plasma/bile concentration ratio was less than 1:1. A significant first‐pass effect was found wh
The present investigation characterized the effect of red kidney bean lectin exposure on gut maturation and function in young piglets. Eleven suckling pigs were given by stomach tube a crude red kidney bean lectin preparation (containing about 25% lectin, 400 mg/kg BW) (lectin-treated pigs) at 10, 11, and 12 d of life, and an additional 16 pigs (control pigs) were given saline instead. On the next
Previous experiments by our group have demonstrated that total parenteral nutrition (TPN) in rats increases the gut permeability to FITC-dextran 70,000 when compared to normally fed controls. TPN may thus, by inducing a 'leaky' gut be one co-factor behind the development of 'gut origin sepsis', a recent hypothesis for the development of multiple organ failure (MOF) in patients subjected to severe
The developmental changes in the ability of the small intestinal epithelium to take up and transfer into blood the macromolecules bovine serum albumin (BSA), bovine immunoglobulin G (BIgG), and fluorescein isothiocyanate-labeled dextran 70,000 (FITC-dextran) were studied in guinea pigs 0-14 days of age. In addition, in the same animals, the activities of the proteases ca-thepsin B and D within the
We report the presence of platelet-activating factor (PAF-acether; l-0-alkyl-2-acetyl-.v«-glycero-3-phosphocholine) in small-intestinal and colonic mucosa of neonatal rats. The PAF-acether content was higher in the colon than in the small intestine, and was lower in the small intestine of 30-day-old animals than in 14-day-old animals. We also report that cultured intestinal epithelial cells (INT 4
C. A. de Wit and B. R. Weström. Purification and characterization of α2-, α2-β- and β-macroglobulin inhibitors in the hedgehog, Erinaceus europaeus: β-macroglobulin identified as the plasma antihemorrhagic factor. Toxicon 25, 1209 - 1219, 1987. - Three macroglobulin inhibitors were purified from hedgehog (Erinaceus europaeus) plasma by sequential chromatography on Cibacron Blue Sepharose, Sephacry
High concentrations of insulin (411 ± 214 μU/ml), neurotensin-like (265 ± 72 pg/ml), and bom-besin-like immunoreactivities (1995 ± 288 pg/ml) were detected in porcine colostrum using radioimmunoassay, as compared to the levels found in sow blood serum at farrowing (5 μU/ml, <12 pg/ml, and 17 pg/ml, respectively). After 72 h of lactation, the levels of insulin and neurotensin-like immunoreactivitie
We have studied the development of phospholipase A2 (PLA2) and lysophosphatidylcholine (lysoPC)-metabolising enzyme activities in the neonatal rat intestine and its relation to the intestinal permeability of macromolecules. The permeability was determined by feeding young rats a mixture of bovine serum albumin, bovine immunoglobulin G and fluorescein-isothiocyanate-conjugated dextran 70,000, and a
Die Charakterisierung der im Schweineserum vorhandenen Inhibitorfraktionen fur Trypsin, Chymotrypsin und Elastase wurde durch Gelfiltration über Ultrogel AcA44 und Aga-rosegelelektrophorese mit anschließender Demonstration der Hemmaktivität der getrennten Fraktionen durchgeführt. Die Inhibitorfraktionen aus der Gelfiltration wurden weiterhin mittels Antikörper identifiziert, die gegen die Protease
We studied the neural and hormonal regulation of the secretion of pancreatic spasmolytic polypeptide (PSP), a potential growth factor, from isolated perfused porcine pancreas and the pancreatic exocrine secretion of PSP in response to a meal in young conscious pigs. PSP concentrations in the pancreatic juice ranged from 1 to 180 μg/ml. PSP released to the venous effluent amounted to 0.4-7% of the
