Microbial hydrogen (H 2) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among the three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted to bacteria and eukaryotes. Here, we show that anaerobic archaea encode diverse, active, and ancient lineages of [FeFe] hydrogenases through combining analysis of exi

Objective: To translate and cross-culturally adapt the Stroke Self-Efficacy Questionnaire (SSEQ) from English to Swedish and to evaluate psychometric properties of the questionnaire. Methods: A cross-sectional study design, where the translation followed a process including initial translation, synthesis, backward translation, expert committee, and pretest. Content validity was assessed using Cont

EXECUTIVE SUMMARY: Microbes are all pervasive in their distribution and influence on the functioning and well-being of humans, life in general and the planet. Microbially-based technologies contribute hugely to the supply of important goods and services we depend upon, such as the provision of food, medicines and clean water. They also offer mechanisms and strategies to mitigate and solve a wide r

A flat-well microformat competitive enzyme-linked immunosorbent chemiluminescent assay for the detection of the pesticide 2,4-dichlorophenoxyacetic acid (2,4-D) is described. Thick-film technology was used to pattern a hydrophobic layer 100 μm thick onto glass microscope slides to form an array of 2 × 2 mm2 squares. These flat wells were able to hold 2 μL of reagents, corresponding to a heig

Background: One of the most profound transitions in healthcare trajectories is when a person turns 18 years old and transfers from child to adult healthcare.Methods: We included all children and adolescents living in Skåne in 2014 and turning 18 years old during the study period. The ID cohort (n=2 072) comprised those with diagnosis of ID and/or service and support for people with ID, and the gen

A NAD+-analog was coimmobilized with lactate dehydrogenase (LDH) on reticulated vitreous carbon (RVC) to give an amperometric lactate biosensor. Both LDH and the NAD+ -analog were bound covalently with carbodiimide to the surface of the porous RVC-material. The electrode was operated in a FIA-arrangement in the presence or absence of a soluble mediator. Meldola Blue. The stability was poor wh

A simple device for the detection of metabolic effects was constructed based on a conducting polymer (polypyrrole) coated with an agarose layer containing immobilised yeast cells. A galvanostatic potentiometric method was used to measure the resistance of the sensor, thus minimising interferences from fluctuations in solution conductivity. The resulting biosensor was used as a simple indicator of

Electrically conducting composite particles were manufactured consisting of a morphine-imprinted methacrylic acid-ethyleneglycoldimethacrylate copolymer interpenetrated with a polypyrrole network. The imprinted polymer was first prepared and then used as a host polymer into which pyrrole monomer was adsorbed. After partial drying of the pyrrole-saturated host polymer, an oxidant was adsorbed, when

Article about the conflicts arising around the rock group Rammstein’s use of German history in general and the Second World War and the Holocaust in particular.

MAX-III is a 700 MeV storage ring for synchrotron radiation that is being built at MAX-lab. The ring will provide radiation in the UV and IR spectral regions. The ring will have a 100 MHz RF system combined with a 500 MHz passive Landau cavity. The lattice is compact, with combined function magnets. The magnets are integrated into the girder providing for ease of alignment.

Different approaches have been used for translation of the EAT–Lancet reference diet into dietary scores that can be used to assess health and environmental impact. Our aim was to compare the different EAT–Lancet diet scores, and to estimate their associations with all-cause mortality, stroke incidence, and greenhouse gas emissions. We did a systematic review (PROSPERO, CRD42021286597) to identify

Objective: Various lifestyle factors including smoking, alcohol, physical activity, sedentary behavior, diet quality, sleep behavior, and overweight have been related to metabolic dysfunction-associated steatotic liver disease (MASLD); however, their joint impact on risk of MASLD is not well known. We prospectively investigated the association between a combination of lifestyle factors and risk of

Enzymatic condensation between amino acids and monosaccharides was studied. A screening involving the OH-containing amino acids serine and threonine and the monosaccharide substrates for the enzymes α-N-acetylgalactosaminidase, α-galactosidase, α-mannosidase, β-xylosidase, and β-galactosidase was carried out. Enzymatic condensation was observed with α-mannosidase and α-N-acetylgalactosaminidase. P

New methods and applications in the separation of biomolecules are reviewed, with an emphasis on the large-scale recovery of proteins. Highlights include the advent of flow-through particles in perfusion chromatography, which allows for very high flow rates, while retaining a high chromatographic efficiency.