Expression, purification and characterization of Violaxanthin De-epoxidase from Spinach
Plants require sunlight to be able to perform photosynthesis but an excess of intense sunlight is harmful to the organism and therefore needs a method to protect itself. Violaxanthin De-Epoxidase (VDE) is located inside the thylakoid lumen and catalyzes the de-epoxidation of violaxanthin to zeaxanthin. Zeaxanthin absorbs the harmful light and dissipates the energy by emitting heat. How this enzyma
