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The Arabidopsis MAP kinase 4 (MPK4) substrate MKS1 was expressed in Escherichia coli and purified, full-length, 6x histidine (His)-tagged MKS1 was phosphorylated in vitro by hemagglutinin (HA)-tagged MPK4 immuno-precipitated from plants. MKS1 phosphorylation was initially verified by electrophoresis and gel-staining with ProQ Diamond and the protein was digested by either trypsin or chymotrypsin f
Nonshivering thermogenesis induced in brown adipose tissue (BAT) during high-fat feeding is mediated through uncoupling protein 1 (UCP1). UCP2 is a recently identified homologue found in many tissues. To determine the role of UCP1 and UCP2 in thermoregulation and energy balance, we investigated the long-term effect of high-fat feeding on mRNA levels in mice at two different ambient temperatures. W
The reaction between pyrite (natural iron (II) disulfide) powder and potassium alkylxanthate has been studied in aqueous solution. Qualitative analysis of the compounds present on pyrite surfaces before and after treatment with ethylxanthate ions under different oxidation conditions has been performed by means of diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy. Atomic absorptio
Since many of the data offered in his text, and many of his conclusions, give the indication of agreeing with what is more or less loosely termed process psychology, I would like to try my own hand at drawing a theoretical sketch of how images can be conceptualized within a theory of perception. The sketch agrees basically with what Quill, inspired by Whitehead, takes to be the requirements of a t
The susceptibility pattern of Mycobacterium marinum was determined. Quinupristin-dalfopristin and telithromycin were less active than clarithromycin. Linezolid showed good antimicrobial activity at clinically achievable concentrations. Gatifloxacin, levofloxacin, and moxifloxacin displayed activities similar to those of ciprofloxacin. Gemifloxacin was less active. The Etest method showed variable
The cubic phase of monoolein (MO) has successfully been used for crystallization of membrane proteins. It is likely that the transition to a lamellar phase upon dehydration is important for the crystallization process, and that the internal dimensions of the lipid phases (i.e., water pore diameter) are crucial for the inclusion and the diffusion of membrane proteins. In the present study, we inves
Our objectives were to compare the prevalence of sexually transmitted infections (STIs) in 103 women undergoing induced legal abortion (LA) and in 101 women with confirmed, recent illegal abortion (IA), in Maputo, Mozambique. For the purpose of this study, LA was considered the abortion provided in the Maputo Central Hospital with the approval of the Ministry of Health, and IA the one not provided
The mobilization of fatty acids from rat and human fat cells is selective according to molecular structure, and notably carbon atom chain length. This study aimed at examining whether the release of individual fatty acids from triacylglycerols (TAG) by hormone-sensitive lipase (HSL) plays a role in the selectivity of fatty acid mobilization. Recombinant rat and human HSL were incubated with a lipi
Mongolian is a language with a rich suffix-based morphology. Underlying forms can contain long consonant strings into which schwa vowels must be epenthesized in order to create well-formed syllables. Syllabification (including epenthesis) is governed by universal principles (the sonority law, maximality, and directionality) and a few language specific rules. Syllabification is cyclic in relation t
These final results from DELPHI searches for the Standard Model (SM) Higgs boson, together with benchmark scans of the Minimal Supersymmetric Standard Model (MSSM) neutral Higgs bosons, used data taken at centre-of-mass energies between 200 and 209 GeV with a total integrated luminosity of 224 pb(-1). The data from 192 to 202 GeV are reanalysed with improved b-tagging for MSSM final states decayin
Identification of risk factors for osteoporosis has been essential for understanding the development of osteoporosis and related fragility fractures. A polymorphism of the binding site for the transcription factor Sp1 of the collagen I alpha 1 gene (COLIA1) has shown an association to bone mass and fracture, but the findings have not been consistent, which may be related to population differences.
Due to increasing demand for software project managers in industry, efforts are needed to develop the management-related knowledge and skills of the current and future software workforce. In particular, university education needs to provide to their computer science and software engineering (SE) students not only technology-related skills but, in addition, a basic understanding of typical phenomen
Symtomen vid diastolisk hjärtsvikt skiljer sig inte från dem vid systolisk funktionsnedsättning. Nya rön har framkommit om förekomsten och betydelsen av symtom och hur de bedöms av patienter och sjukvårdspersonal. Bakomliggande mekanismer till diastolisk rubbning presenteras här utifrån cellulära mekanismer och bakomliggande sjukdomar såsom hypertoni, diabetes och övervikt.
The story of how conflict prevention became an integral and legitimate part of Swedish foreign policy illustrates the relationship between successful practices and powerful ideas. This article suggests that the demonstration of an idea in practice empowers the idea and contributes not only to its selection, but also to its framing and institutionalization within foreign policy. Hence, the article
Current state of the art suggests the use of variational formulations for solving a variety of computer vision problems. This thesis deals with such variational problems which often include the optimization of curves and surfaces. The level set method is used throughout the work, both as a tool in the theoretical analysis and for constructing practical algorithms. One frequently occurring example
Artikel om efterkrigstidens bruk av förintelsen.
Hormone-sensitive lipase (HSL) is expressed and enzymatically active in beta-cells and has been proposed to be involved in the generation of the lipid-derived signal that seems to be necessary for glucose-stimulated insulin secretion. In this study, we investigated whether the expression of HSL in INS-1 cells and in rat islets is affected by exposure to high glucose concentrations. Incubation of I
Oxidative stress, or the production of oxygen-centered free radicals, has been hypothesized as the major source of DNA damage that in turn can lead to altered genetic expression, disease, and aging of humans. Serum protein thiol levels in blood are a direct measure of the in vivo reduction/oxidation (redox) status in humans, because thiols react readily with oxygen-containing free radicals to form
