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Macromolecular crowding and its conequences

Incompatible pairs of polymers separate into two phases in aqueous solution above a few percentage points total concentration. Protein pairs can also produce phase separation, but at somewhat higher concentrations. In this chapter, we explore the effect of high background concentrations of macromolecules on phase separation of pairs of species which would not be at sufficiently high concentration

Beyond neopositivists, romantics, and localists: A reflexive approach to interviews in organizational research

In this article I develop a framework for thinking about the research interview, and I critique dominating neopositivist and romantic views on the interview. I suggest eight metaphors that offer reconceptualizations of the interview, drawing upon recent theoretical trends on language, the subject, and discourse, and develop a reflexive theoretical framework. The article provides new ways of dealin

Antigen-independent binding of rat immunoglobulins in a radioimmunoassay. Solutions to an unusual background problem

A high non-specific binding of immunoglobulins to plastic surfaces was noted with a number of rat sera, when tested in an indirect 125I-labeled protein-A assay for detection of cell-surface-bound rat immunoglobulins of various classes and IgG subclasses. This type of non-specific binding was found with all types of Ig. The degree of binding varied with the type of test plate used and fluctuated wi

Isoform-specific regulation of mood behavior and pancreatic beta cell and cardiovascular function by L-type Ca2+ channels

Ca(v)1.2 and Ca(v)1.3 L-type Ca2+ channels (LTCCs) are believed to underlie Ca2+ currents in brain, pancreatic beta cells, and the cardiovascular system. In the CNS, neuronal LTCCs control excitation-transcription coupling and neuronal plasticity. However, the pharmacotherapeutic implications of CNS LTCC modulation are difficult to study because LTCC modulators cause card iovascular (activators an

Molecularly imprinted materials: Towards the next generation

This brief overview summarizes some recent developments from our Center for Molecular Imprinting related to the topic of this symposium. After a short presentation of the principle of molecular imprinting and recognition, the use of different materials including hybrids for the formation of the host will be discussed, followed by examples given of different formats used such as small polymer beads

Biosensor discrimination of meat juice from various animals using a lectin panel and ellipsometry

In this work, simple microcontact printed gold-wafers were used to make a lectin panel for investigation and discrimination of different meat juices from fresh meat of cattle, chicken, pig, cod, turkey and lamb. Seven different lectins were thus attached to gold surfaces using the streptavidin–biotin method. Lectins recognize and bind specifically to carbohydrate structures present on different pr

Effects of montelukast and salmeterol on physical performance and exercise economy in adult asthmatics with exercise-induced bronchoconstriction

STUDY OBJECTIVES: To compare the effect of montelukast and the long-acting beta(2)-agonist salmeterol on cardiopulmonary exercise economy and physical performance in adult patients with asthma during exercise. DESIGN AND PATIENTS: Asthmatic patients (n = 18), aged 18 to 35 years with exercise-induced bronchoconstriction (EIB), using a double-blind, double-dummy cross-over design. Montelukast, 10 m

Novel aspects of glypican glycobiology.

Mutations in glypican genes cause dysmorphic and overgrowth syndromes in men and mice, abnormal development in flies and worms, and defective gastrulation in zebrafish and ascidians. All glypican core proteins share a characteristic pattern of 14 conserved cysteine residues. Upstream from the C-terminal membrane anchorage are 3–4 heparan sulfate attachment sites. Cysteines in glypican-1 can become

Plasminogen is a critical host pathogenicity factor for group A streptococcal infection

Group A streptococci, a common human pathogen, secrete streptokinase, which activates the host's blood clot-dissolving protein, plasminogen. Streptokinase is highly specific for human plasminogen, exhibiting little or no activity against other mammalian species, including mouse. Here, a transgene expressing human plasminogen markedly increased mortality in mice infected with streptococci, and this

Detection of Helicobacter species in liver and stomach tissues of patients with chronic liver diseases using polymerase chain reaction-denaturing gradient gel electrophoresis and immunohistochemistry

Objective. Helicobacter DNA has been detected in the hepatobiliary tree of patients with chronic liver diseases (CLD). The presence of H. pylori in the stomach compared with in the liver of the same patients with CLD has not been studied, therefore to the aim of this study was to investigate the presence of Helicobacter DNA and antigens in the liver and stomach of Polish patients with chronic live

Åtgärdsprogram för bevarande av flikstånds (Senecio erucifolius)

Flikstånds (Senecio erucifolius) är mycket sällsynt och rödlistad som Akut hotad i Sverige (Gärdenfors 2005). Den förekommer i gräsbevuxna fältkantzoner, vägkanter och sluttningar på jordbruksmark i Skåne. Även om många lokaler rapporterats i Skåne så är endast två lokala populationer kända idag, varav en minskar i storlek. De största hoten utgörs av habitatförstörelse (genom intensifierat jordbru

Signal alterations, artifacts and image distortion induced by a superparamagnetic contrast medium. A phantom study in a 0.3 tesla MR system

In a phantom study using a 0.3 tesla MR system the signal alterations, artifacts and image distortion induced by a bowel contrast medium containing superparamagnetic magnetite particles were evaluated. A concentration of 20 mg iron/l was most useful because it caused a significant signal reduction without disturbing artifacts and image distortion. The signal reduction was most pronounced on long T