Enhancing the productivity of the bi-enzymatic convergent cascade for ɛ-caprolactone synthesis through design of experiments and a biphasic system
A two-step Design of Experiments (DoE) strategy followed by a two-liquid-phase system (2LPS) was applied to enhance the ɛ-caprolactone yield in the cyclohexanone monooxygenase (CHMO)-alcohol dehydrogenase (ADH) convergent cascade system. The key reaction parameters were identified and optimized for the determination of an optimal operational window for the aqueous media. In the 2LPS system, high p