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Presently, unrestrained plethysmography is widely used to assess bronchial responsiveness in mice. An empirical quantity known as enhanced pause is derived from the plethysmographic box pressure [P-b( t), where t is time] and assumed to be an index of bronchoconstriction. We show that P-b(t) is determined largely by gas conditioning when normal mice breathe spontaneously inside a closed chamber in
