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54 human genes were selected as test targets for parallel cloning, expression, purification and crystallization. Proteins from these genes were selected to have a molecular weight of between 14 and 50 kDa, not to have a high percentage of hydrophobic residues (i.e. more likely to be soluble) and to have no known crystal structures and were not known to be subunits of heterocomplexes. Four proteins
Nuclear structure studies at CST attracted recently increased interest for the results of present activities as well as for the future project FAIR. A broad range of physics phenomena can be addressed by high-resolution in-beam gamma-ray spectroscopy experiments with radioactive beams offered within the Rare Isotopes Spectroscopic INvestigation at GSI (RISING) project. It combined the EUROBALL Ge-
Falling birth rates within the European Union have given rise to a number of studies, trying to analyse and interpret why women (and men) choose not to have (many) children. One attempt in this direction is made by an ongoing EU-project, involving eight countries, including Sweden. Qualitative interviews focusing on couples' decisions to have- or not have - children have been carried out in each c
Two industrial effluents, a pre-fermentation effluent and a post-fermentation effluent from a wheat starch production plant, were used as substrates for fuel ethanol production in anaerobic batch cultures using minimal nutritional amendment. The performances of three metabolically engineered xylose-utilizing Saccharomyces cerevisiae strains: TMB 3001 expressing XYL1, XYL2 and XKS1, redox metabolis
Popular Abstract in Swedish Populärvetenskaplig sammanfattning Det magnifika skådespelet av erupterande geysrar, varma, rykande källor av skilda slag samt kokande lerpölar är en uppseendeväckande syn. Dessa extrema miljöer av hög temperatur och mycket låga pH-värden verkar långt ifrån livsbejakande. Trots detta finns liv representerat i form av mikroorganismer som här har funnit sina ideala livsmAbstract Hot springs in Iceland can serve as sources of thermophilic, xylanolytic bacteria secreting very thermostable xylanolytic enzymes. The aerobic, thermophilic bacteria Rhodothermus marinus secretes multiple xylanases and xylosidases. One of the xylanases was purified to electrophoretic homogeneity and was characterized. The N-terminal sequence showed no homology with other xylanases. The p
An automated glucose feeding strategy that avoids acetate accumulationin cultivations of Escherichia coli is discussed. We havepreviously described how a probing technique makes it possible todetect and avoid overflow metabolism using a dissolved oxygen sensor.In this paper these ideas are extended with a safety net thatguarantees that aerobic conditions are maintained. The method isgenerally appl
Upon disturbance of their function by stress, mitochondria can signal to the nucleus to steer the expression of responsive genes. This mitochondria-to-nucleus communication is often referred to as mitochondrial retrograde regulation (MRR). Although reactive oxygen species and calcium are likely candidate signaling molecules for MRR, the protein signaling components in plants remain largely unknown
The devices that constitute the Internet of Things are evolving to include more than just enabling sensing and actuation over a wireless interface. In a contemporary scenario, these devices perform tasks and contribute to an aggregate information flow, in a distributed manner. In the wake of this evolution, new distributed Internet of Things frameworks have emerged. These frameworks maintain a dis
Background--Atherosclerosis is an inflammatory lipid disorder and the main underlying pathology of acute ischemic events. Despite a vast amount of data from murine atherosclerosis models, evidence of B-cell involvement in human atherosclerotic disease is limited. We therefore investigated the association of circulating B-cell subtypes with the occurrence of secondary cardiovascular events in advan
Abstract in UndeterminedProfessor Mylly suggests that the Lisbon Treaty opens a new avenue for the Commission when the internal legislative pathway becomes too complicated, demanding unanimity in decision-making. An alternative route would be to use the competence to enter into international agreements, where qualified majority is enough. The agreements may be phrased in such a way that they have
This discussion paper, by a Working Group of INTIMATE (Integration of ice-core, marine and terrestrial records) and the Subcommision on Quaternary Stratigraphy (SQS) of the International Commission on Stratigraphy (ICS), considers the prospects for a formal subdivision of the Holocene Series/Epoch. Although previous attempts to subdivide the Holocene have proved inconclusive, recent developments i
Offentliga uppgifter och verksamheter utförs inte bara av statliga och kommunala myndighetsorgan utan även genom andra fristående rättssubjekt, såsom bolag, korporationer och enskilda. Det allmännas skadeståndsansvar påverkas först och främst av om det utgör myndighetsutövning, vilket bland annat ställer krav på verksamhetens karaktär och anknytning till ett myndighetsärende. Även utanför detta om
The Philippines is a unique case to study national identity as it’s a post-colonial, highly Americanized country constituted by quantities of ethnic groups. Filipino identity emerged from the revolution against Spanish colonization and developed as an anti-colonial movement along with its struggles for independence. However, after independence, the Philippines has been facing a dilemma that, on on
