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Development of Methodology to Investigate the Surface SMALPome of Mammalian Cells

Extraction of membrane proteins from biological membranes has traditionally involved detergents. In the past decade, a new technique has been developed, which uses styrene maleic acid (SMA) copolymers to extract membrane proteins into nanodiscs without the requirement of detergents. SMA nanodiscs are compatible with analytical techniques, such as small-angle scattering, NMR spectroscopy, and DLS,

The Gaia -ESO Survey : The analysis of the hot-star spectra

Context. The Gaia-ESO Survey (GES) is a large public spectroscopic survey that has collected, over a period of six years, spectra of ~105 stars. This survey provides not only the reduced spectra, but also the stellar parameters and abundances resulting from the analysis of the spectra. Aims. The GES dataflow is organised in 19 working groups. Working group 13 (WG13) is responsible for the spectral

Biologics in severe asthma : A pragmatic approach for choosing the right treatment for the right patient

The development of monoclonal antibody therapies targeting specific components of the pathways relevant to asthma pathophysiology has revolutionized treatment of severe asthma both in adults and children and helped to further unravel the heterogeneity of this disease. However, the availability of multiple agents, often with overlapping eligibility criteria, creates a need for pragmatic guidance fo

Immune thrombocytopenia : Pathophysiology and impacts of Romiplostim treatment

Immune thrombocytopenia (ITP) is an autoimmune bleeding disease caused by immune-mediated platelet destruction and decreased platelet production. ITP is characterized by an isolated thrombocytopenia (<100 × 109/L) and increased risk of bleeding. The disease has a complex pathophysiology wherein immune tolerance breakdown leads to platelet and megakaryocyte destruction. Therapeutics such as cortico

A Hybrid Cuk PFC Converter for 48 V – 12 Ah Battery Charging Application

Power Factor Correction (PFC) converters are essential in designing any charging architecture, regardless of the battery's capacity and the application. Nevertheless, power converters, having continuous output current features, are widely used for battery charging applications. In contrast, converters with continuous input current characteristics exhibit better PFC performance. The Cuk converter c