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A PCR/restriction digestion assay for the detection of the transcript variants 1 and 2 of POU5F1.

POU5F1 has two alternatively spliced transcripts: a long (variant 1, NM_002701) and a short (variant 2, NM_203289) transcript. Only variant 1 is a key regulator of pluripotency. Hence, it is important to be able to distinguish this transcript from variant 2 and from the many pseudogenes present in the genome. Previous studies on the expression of POU5F1 were, however, usually carried out without c

Mammalian Molecular Systematics with Emphasis on the Insectivore Order Lipotyphla

Insectivore classification has been one of the most controversial problems in mammalian systematics. Evolutionary relationships among the six extant families, Talpidae (moles), Soricidae (shrews), Erinaceidae (hedgehogs), Tenrecidae (tenrecs), Chryosochloridae (golden moles), and Solenodontidae (solenodons), have remained uncertain based on morphological data. Animals classified within the order L

Low or No Inhibitory Potency of the Canonical Galectin Carbohydrate-binding Site by Pectins and Galactomannans

Some complex plant-derived polysaccharides, such as modified citrus pectins and galactomannans, have been shown to have promising anti-inflammatory and anti-cancer effects. Most reports propose or claim that these effects are due to interaction of the polysaccharides with galectins because the polysaccharides contain galactose-containing side chains that might bind this class of lectin. However, t

Skådespelarens musikalitet

This investigation takes its point of departure in the prominent use of the term ’musicality’ as a prestige word in theatre discourse where, obviously, not exactly the same reference and meaning is ascribed to the concept as in contexts of music. There seems to be very little previous research in this area, if any. The research question is formulated thus: What do theatre practicians mean when the

The Scandinavian Sarcoma Group Central Register : 6,000 patients after 25 years of monitoring of referral and treatment of extremity and trunk wall soft-tissue sarcoma

Purpose — We wanted to examine the potential of the Scandinavian Sarcoma Group (SSG) Central Register, and evaluate referral and treatment practice for soft-tissue sarcomas in the extremities and trunk wall (STS) in the Nordic countries. Background — Based on incidence rates from the literature, 8,150 (7,000–9,300) cases of STS of the extremity and trunk wall should have been diagnosed in Norway,

‘What Local People?’ : the establishment of a mine in Kallak, Jokkmokk, understood from a stakeholder recognition framework

Global exploration and production of minerals is increasingly located in areas populated by indigenous people. Conflicts that arise between multinational corporations and local activists and/or indigenous people are widespread while the understanding of such issues are still lacking. The Swedish government encouraging an expanding mining industry have caused great resistance, particularly due to t

Cannabis i Sverige och Portugal - En komparativ studie mellan två olika drogstrategier

Narkotikapolitik är idag ett väldiskuterat och aktuellt ämne. Lagstiftning skiljer sig väsentligt mellan länder och likaså idéer och tankar kring vilken väg som är rätt att gå. Syftet med denna uppsats är granska samt synliggöra Portugals och Sveriges vitt skilda narkotikapolitik för att genom teori analysera och redogöra för ländernas motiv, resultat och påverkan på samhället samt individ. En visDrug policies are today a deeply discussed subject and types of legislation, ideas and thoughts differ from country to country. The purpose of this paper is to examine the widely different narcotic policies of Portugal and Sweden, as a means to through sociological theory analyze and portray the different motives, results and effects on society and the individual. Both Sweden and Portugal use soci

Developing a spiking neural model of Long Short-Term Memory architectures

Current advances in Deep Learning have shown significant improvements in common Machine Learning applications such as image, speech and text recognition. Specifically, in order to process time series, deep Neural Networks (NNs) with Long Short-Term Memory (LSTM) units are widely used in sequence recognition problems to store recent information and to use it for future predictions. The efficiency i

Frihet att välja - så länge du väljer rätt. En studie av religionsfriheten i 1809 års regeringsform

1809 antogs en ny regeringsform i Sverige. En nyhet i denna var att den ålade kungen att skydda var och ens rätt till fri religionsutövning, vilket på ytan verkar innebära att religionsfrihet infördes. Samtidigt fanns det i kyrkolagen och missgärningsbalken regler som innebar att svenska medborgare var förbjudna att konvertera från den luthersk-evangeliska kristendomen. Det till synes paradoxala fSweden adopted a new constitution in 1809. It included an article that obligated the king to protect each and everyone’s right to freely practice their religion. This seems like an introduction of religious freedom into Swedish law. However, general law prohibited Swedish citizens from deviating from lutheranian christianity. This thesis focuses on the seemingly contradictory relationship between

Extending the water life cycle in rural South African households: the role and potential of water reuse solutions

Despite the issue of greywater management gaining more importance around the world, espe-cially in developing countries, it still remains a challenge in non-sewered areas of South Afri-ca. For a semi-arid country, reusing greywater on a household level has great potential as an alternative water source if done properly. On a household level, the popular repurposing of greywater is for irrigation

Förödmjukande uppdrag - Om journalisters möjlighet att arbetsvägra

Arbete i utbyte mot lön utgör grundförutsättningarna för i snart sagt allt ar-bete. Den som bryter denna överenskommelse löper risk att göra sig skyldig till arbetsvägran och i värsta fall bli avskedad. Kort sagt: du bör göra som du blir tillsagd på ditt arbete. I de kollektivavtal som finns mellan Svenska Journalistförbundet och arbets-givarorganisationen Almega finns dock en regel som öppnar upLabour in exchange of salary is the basic agreement of almost all em-ployments. A person who violates this agreement will be at risk of being guilty of refusal of work and, in worst case, could be dismissed. In other words: You should do what you are told at work. However, the collective agreements that exist between the Swedish Journalist Union and the employers organization Almega contains a ru

Performance of frozen meal packaging system in the cold supply chain

Packaging helps to protect the product and safety handover the product from producer to end user. When the packaging leaves the closed loop it creates environmental issues. Also, the material focused packaging development process creates trade-off among supply chain actors. The sustainable packaging would reduce the overall cost and environmental impact, increase the value of the packed product in

Building a circular economy

The construction sector is one of the biggest contributors to global CO2 emissions and buildings account for a significant part of the energy use in Sweden. Population growth in combination with economic development is putting more pressure on the environment, resulting in escalating consumption trends and larger quantities of raw material extraction. Policies and directives are promoting a circul

Vad kallas det när bomberna faller? En studie av det straffrättsliga terrorismbegreppet mot bakgrund av legalitetsprincipen

Även om terrorism utgör ett växande problem kan få presentera en precis och heltäckande förklaring av dess innebörd. I en internationell såväl som akademisk kontext råder oenighet om vilka handlingar som bör omfattas. I svensk straffrätt saknas likaså en definition av begreppet; istället straffbeläggs ett stort antal terroristhandlingar under skilda brottstyper. Uppsatsens syfte är att utreda vadAlthough terrorism poses a growing threat, few can present a precise and all encompassing definition of the term. Internationally, as well as academically, there is disagreement about which acts should be subjected by the term. Swedish criminal law similarly lacks a definition; instead numerous terrorist acts are criminalized under separate forms of crimes. The purpose of this essay is to investi

Tally, ett LCA-verktyg i BIM

I år, 2020 har ett lagförslag från Boverket angående krav på klimatdeklarationer lämnats på remiss. Lagförslaget handlar om att fastställa ett krav på byggherrar, att vid nybyggnationer inkludera en klimatdeklaration. Denna klimatdeklaration ska verkställas genom att utföra en livscykelanalys på hela nybyggnationens klimatskal, stomme och innerväggar. För att kunna förenkla arbetet med klimatkalkyThis year, 2020, a bill from the Swedish National Board of Housing, Building and Planning regarding requirements for climate declarations has been submitted on a referral. The bill is about establishing a requirement for builders, to include a climate declaration for new buildings. This climate declaration is to be implemented by performing a life-cycle analysis of the entire new building's cl

Lag och ordning – om avgränsningen av det straffbara området för 16 kap. 3 § BrB utifrån legalitetsprincipen och skyddet för mötes- och demonstrationsfriheten

Att störa allmän ordning, en av brottsförutsättningarna för ohörsamhet mot ordningsmakten i 16 kap. 3 § brottsbalken, är erkänt oklart. Rättsfallet NJA 1989 s. 308, vilket har prejudikatstatus vad gäller tolkningen av störande av allmän ordning, innebar en extensiv tolkning av ordningsbegreppet och har fått kritik från statsrättsligt håll för bristande avvägning gentemot mötes- och demonstrationsfDisturbing public order, one of the conditions of criminal liability for refusing to obey an official command under Chapter 16, Section 3, of the Swedish Criminal Code, is admittedly obscure. The case NJA 1989, p. 308, which established a precedent regarding the interpretation of disturbing public order, entailed an extensive understanding of the concept of order. The judgement has been criticised

Medellösa processubjekt i frontlinjen - En civilprocessuell studie av ersättningsgilla rättegångskostnader i ljuset av tredjepartsfinansiering

En huvudprincip i den svenska rättsordningen är att den förlorande parten i ett tvistemål ska ersätta den vinnande parten för dennes rättegångskostnader. Ersättningen till den vinnande parten ska i enlighet med 18 kap. 8 § rättegångsbalken (1942:740), fullt motsvara kostnaderna för processföringen, såvitt kostnaderna skäligen varit påkallade för tillvaratagande av partens rätt. Inom ramen för part

Livsmedelsverksamheters allergeninformation ur ett konsumentperspektiv -En uppföljning av ett allergenprojekt inom den offentliga livsmedelskontrollen

Stor risk för mjölkallergiker att äta på restaurang i Malmö Vågar allergiker äta på restaurang? Går det att lita på att maten innehåller det som restaurangpersonalen säger att den gör? Innehåller grädde, ost och yoghurt mjölk? Att äta på restaurang kan vara utmanande för en allergiker eftersom restauranger är bland de vanligaste platserna där allergiska reaktioner inträffar. Omkring 15 procentFoodallergy is on the rise in Sweden and the rest of the world and is a global health issue. Milk protein is the allergen that causes the most allergic reactions in Sweden and those who are highly allergic to milk risk going into an anaphylactic shock which in worst case can be fatal. Eating at a restaurant can be a challenge for a person with food allergy since this is where the majority of aller