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Today almost every system or service, e.g., water, power supply, transportation, etc. is dependent on IT systems, and failure of these systems have serious and negative effects on the society. IT incidents are critical for the society as they can stop the function of critical systems and services. Moreover, in a software engineering context risk analysis is an important activity for the developmen
Abstract in UndeterminedIn this paper we describe and reflect upon a novel methodology using attention cards to facilitate communication, concentration and creativity in a user requirement workshop with seniors. Previous research indicates that seniors tend to lose focus and start cross talking during workshops, which results in broad and shallow findings. However, our findings indicate that the u
The linear ubiquitin chain assembly complex (LUBAC) is required for optimal gene activation and prevention of cell death upon activation of immune receptors, including TNFR1 1 . Deficiency in the LUBAC components SHARPIN or HOIP in mice results in severe inflammation in adulthood or embryonic lethality, respectively, owing to deregulation of TNFR1-mediated cell death 2-8 . In humans, deficiency in
Aims: Immobilization of microbial cells is a useful strategy for developing high cell density bioreactors with improved stability and productivity for production of different chemicals. Functionalization of the immobilization matrix or biofilm forming property of some strains has been utilized for achieving cell attachment. The aim of the present study was to investigate the production of exopolys
Background: Reproducible quantification of drug transporter protein expression in tissues is important for predicting transporter mediated drug disposition. Many mass-spectrometry based transporter protein quantification methods result in high variability of the estimated transporter quantities. Therefore, we aimed to evaluate and optimize mass spectrometry-based quantification method for drug tra
Aim: To test the hypothesis that a brief window of time immediately after delivery may be a particularly sensitive period for olfactory learning by human neonates. Methods: Fifty-five vaginally delivered newborns were exposed to an odorant for 30 min beginning 4-37 min after birth (Early exposure) or 12-h post-partum (Late exposure). Several days later, newborns' head orientation responses to the
We consider least-squares approximation of a function of one variable by a continuous, piecewise-linear approximand that has a small number of breakpoints. This problem was notably considered by Bellman who proposed an approximate algorithm based on dynamic programming. Many suboptimal approaches have been suggested, but so far, the only exact methods resort to mixed integer programming with super
The use of plastic scintillation microspheres (PSm) is an innovative technique for measuring the radioactivity of beta-emitting radionuclides. PSm can be produced via different methods; none of which has been assayed at the industrial scale. In the present paper, we evaluate the production of PSm by spray-drying on an industrial scale. Our results indicate that fluorescent solutes were indeed enca
Dutch elm disease (DED), caused by the fungi Ophiostoma ulmi and O. novo-ulmi, has reduced elm populations severely in Europe and North America. Breeding programmes are in action to find less susceptible elm varieties suitable for re-establishing elm stands. Bark beetles, mainly Scolytus spp., are the only known natural vectors of DED. During twig feeding, beetles transfer Ophiostoma spores to hea
Mass spectrometry has emerged as a core technique for protein identification and characterization because of its high sensitivity, accuracy, and speed of analysis. The most widespread strategy for studying global protein expression in biological systems employs analytical two-dimensional polyacrylamide gel electrophoresis (2D PAGE) followed by enzymatic degradation of isolated protein spots, pepti
Since the 1990s, the low number of students choosing to study science and technology in higher education has been on the societal agenda and many initiatives have been launched to promote awareness regarding career options. The initiatives particularly focus on increasing enrolment in the engineering programmes. This article describes and compares eight European initiatives that have been establis
Genome-wide association studies have identified risk loci for SLE, but a large proportion of the genetic contribution to SLE still remains unexplained. To detect novel risk genes, and to predict an individual's SLE risk we designed a random forest classifier using SNP genotype data generated on the "Immunochip" from 1,160 patients with SLE and 2,711 controls. Using gene importance scores defined b
BACKGROUND: A department of forensic medicine (DFM) can be a valuable source for tissue donation, but logistics can prove difficult to overcome as it pertains to obtaining tissues for donation. This article describes the potential of tissues that can be procured for transplantation.MATERIALS AND METHODS: Sweden has 9.7 million inhabitants, with an annual mortality rate of 90 000 and 5500 medicoleg
Background: Amino acid substitutions due to DNA nucleotide replacements are frequently disease-causing because of affecting functionally important sites. If the substituting amino acid does not fit into the protein, it causes structural alterations that are often harmful. Clashes of amino acids cause local or global structural changes. Testing structural compatibility of variations has been diffic
A novel and generally applicable method for determining structures of membrane proteins in solution via small-angle neutron scattering (SANS) is presented. Common detergents for solubilizing membrane proteins were synthesized in isotope-substituted versions for utilizing the intrinsic neutron scattering length difference between hydrogen and deuterium. Individual hydrogen/deuterium levels of the d
Fabrication and application of a non-fluorescent and UV-transparent microfluidic biochip in fused silica that allows sensitive autofluorescence detection are described. The biochip is particularly useful in cell-based assays where the most informative autofluorescence signals from the cells reside in the ultraviolet spectral range and where plastic labware materials commonly used in cell culture w
Pickering emulsion polymerization was used to synthesize molecularly imprinted polymer beads for the selective recognition of 17-beta-estradiol under aqueous conditions. Scanning electron microscopy analysis indicated that the imprinted polymer beads had a small diameter with a narrow size distribution (18.9 +/- 2.3 mu m). The reduction in particle size achieved in this study was attributed to the
We present here an innovative, fluorescent, monoolein-based cubosome dispersion. Rather than embedded within the monoolein palisade, the fluorescent imaging agent, namely dansyl, was conjugated to the terminal ethylene oxide moieties of the block copolymer Pluronic F108. We discuss the physicochemical and photophysical properties of this fluorescent Pluronic and of a cubosome formulation stabilize
