Sökresultat

Proteins carrying genetically attached polyhistidine tails have been purified using affinity precipitation with metal chelates. DNA fragments encoding four or five histidine residues have been genetically fused to the oligomeric enzymes lactate dehydrogenase (Bacillus stearothermophilus), beta-glucoronidase (Escherichia coli), and galactose dehydrogenase (Pseudomonas fluorescens) as well as to the

The design of an in vitro immunization system based on a synthetic heterotope immunogen, which was a peptide containing both T- and B-cell epitopes, that elicited a neutralizing, primary human humoral immune response against the human immunodeficiency virus (HIV-1) is reported here. This heterotope construct contained the major neutralizing B-cell epitope, within the V3 region of glycoprotein 120

Low-energy-electron-diffraction (LEED), scanning-tunneling-microscopy (STM), and photoelectron-spectroscopy measurements have been performed on the ordered submonolayer surface reconstructions of Yb on Si(111). Two of these reconstructions, namely, 3×1 and 2×1, have been studied in detail. STM and LEED revealed that what was considered to be the 3×1 reconstruction is actually a 3×2 reconstruction.

Policy intervention schemes for increased deployment of solar photovoltaics (PV) have been launched in several countries, with varying success. In order to ensure an efficient deployment of the technology, thorough knowledge is needed about relevant present actors and institutions, and about how a desirable actor base and institutional setup should look like; mere cost reductions are not enough to

Protein S is a vitamin K-dependent plasma protein. It functions as a cofactor to activated protein C in the inactivation of factors Va and VIIIa by limited proteolysis. Protein S is very sensitive to proteolysis by thrombin which reduces its calcium ion binding and leads to a loss of its cofactor activity. We have now determined the sequence of the 100 amino-terminal amino acid residues and locali

Detaljerade data är avgörande vid karaktärisering av komplexa förorenade områden. I denna studie utrustar vi ett antal G360-brunnssystem med Star-Oddi milli-TD-tryckgivare i ett mestadels PCE (tetrakloretylen)- och TCE (trikloretylen)-förorenat bostadsområde i Helsingborg. Trots att dessa tryckgivare vanligtvis används inom marina undersökningar gör deras kostnad samt kompakta storlek dem av intre

In this report the preparation of colloidal molecules through electrostatically driven self-assembly of spherical, thermoresponsive microgel building blocks is investigated. Colloidal molecules are of interest to the scientific community because, unlike spherical colloids, they account for anisotropic interactions that can also be found in atomic molecules. The added benet of using thermoresponsiv

Populärvetenskaplig sammanfattning: Då man för ner en elektrisk ström i marken kommer vissa material i marken laddas upp p.g.a. att positiva och negativa joner samlar ihop sig på ytor och i trånga porkanaler. Detta kallas för polarisering och kan användas för att bestämma t.ex. vad för typ av geologiska material som finns under markytan eller vart föroreningar i marken tar vägen. Fenomenet som uppIt has been shown that surveys of Induced polarization (IP) often contain data of low quality, because it is highly sensitive against different noise sources. When measuring induced polarization, where the method uses multicore cables and continuous electrode sounding (CVES, Continuous Vertical Electrical Sounding), noise phenomena such as capacitive coupling effects have to be considered. It is p

Popular summary Energy generation from renewable sources is one of the main issues to be solved for the 21st century. One of the most advanced technologies currently available is wind energy, which has a large potential onshore and offshore. However, in many cases wind energy is restricted to large wind parks with wind power plants sometimes exceeding heights of 100 m. The construction of large wi

Denna studie undersöker den rumsliga variationen i markens koldioxid-utflöde (CO2) i en boreal blandskog, huvudsakligen dominerad av tall (Pinus sylvestris L.) och gran (Picea abies L.) i ADVEX experimentsite i Norunda, centrala Sverige. Mätningar av markens CO2 gjordes i 72 collars omkring huvudtornet med hjälp av en LI-6250 infraröd gasanalysapparat kopplad till en hemmagjord mörkkammare. Medelv

Semi-natural grasslands in Scandinavia have been created by humans since many millennia ago, but the agricultural intensification in the last few centuries caused them to decline heavily. Accordingly, the biodiversity associated to these open, nutrient-poor habitats declined as well and many remnant populations are slowly declining and threatened to go extinct. In this study, I focused on declin

Over the past decade, organic–inorganic hybrid perovskites have revolutionized next-generation semiconductors, driving unprecedented advancements in cost-effective optoelectronics. While lead-based perovskite semiconductors exhibit exceptional optoelectronic properties, their inherent toxicity and vulnerability to environmental degradation remain significant barriers to widespread commercializ

We investigated whether local biotic and abiotic conditions could explain the occurrence of calling males of the declining spadefoot toad Pelobates fuscus in 72 ponds in southern Sweden. The ponds covered the entire distribution range of P. fuscus and were monitored during the breeding season in 2000. Calling males were found in 33 ponds. representing ca 50% of all known ponds for the species ill