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Baculovirus-insect cell expression system has become one of the most widely used eukaryotic expression systems for heterologous protein production in many laboratories. The availability of robust insect cell lines, serum-free media, a range of vectors and commercially-packaged kits have supported the demand for maximizing the exploitation of the baculovirus-insect cell expression system. Naturally
New Findings: What is the central question of this study? Collagen-binding β1-integrins function physiologically in cellular control of dermal interstitial fluid pressure (PIF) in vivo and thereby participate in control of extravascular fluid volume. During anaphylaxis, simulated by injection of compound 48/80, integrin αVβ3 takes over this physiological function. Here we addressed the question wh
Effective societal responses to rapid climate change in the Arctic rely on an accurate representation of region-specific ecosystem properties and processes. However, this is limited by the scarcity and patchy distribution of field measurements. Here, we use a comprehensive, geo-referenced database of primary field measurements in 1,840 published studies across the Arctic to identify statistically
Languages borrow words when there is a need for it, all languages contain loanwords, and no part of the lexicon is entirely “loan-proof”. These are statements about lexical borrowing that are typically found in linguistic textbooks (Hock and Joseph 1996). Further, we know that that there are large discrepancies in the borrowability of different lexical concepts, where core vocabulary domains (sens
The recent more or less complete return and recovery in Skåne, southern-most Sweden, of epiphytic bryophyes known to have suffered from atmospheric pollution in the mid 1900’s, e.g. Zygodon rupestris, Antitrichia curtipendula, Leucodon sciuroides, Orthotrichum lyellii, O. speciosum and O. stramineum, is here discussed and illustrated on maps. However, apart from these successful recoveries, at lea
Mesenchymal stromal cells (MSC) are ideal candidates for cell therapies, due to their immune-regulatory and regenerative properties. We have previously reported that lung-derived MSC are tissue-resident cells with lung-specific properties compared to bone marrow-derived MSC. Assessing relevant molecular differences between lung-MSC and bone marrow-MSC is important, given that such differences may
Prolidase is a ubiquitously distributed dipeptidase and the only dipeptidase in humans capable of cleaving the peptide bond preceding the amino acids proline (Pro) or hydroxyproline (Hyp). It is mainly implicated in the degradation of dietary and endogenous proteins. It is also involved in the terminal steps of collagen catabolism by hydrolyzing Pro and Hyp-containing dipeptides. Finally, it is be
The institution of energy consulting services is one of the several government instruments for improving the efficiency of energy usage in many European countries. Various forms of advice on energy issues are offered to households. In Sweden, there has been a drop in the number of households who avail of this service, even though it is offered independently, free of charge and easily accessible. I
The particle number size distribution is an important parameter to characterize the atmospheric aerosol and its influence on the Earth's climate. Here we describe a new optical particle size spectrometer (OPSS) for measurements of the accumulation mode particle number size distribution in the tropopause region on board a passenger aircraft (IAGOS-CARIBIC observatory: In-service Aircraft for a Glob
Molecularly well-designed proton exchange membranes (PEMs) with a high local concentration of strongly acidic groups have the potential to fulfill the strict requirements for fuel cell operation under high temperature and low humidity. Here, we have prepared a series of well-defined and tunable PEMs based on poly(p-phenylene alkylene) backbones functionalized with sulfonated polypentafluorostyrene
Cyclic chiral lactide is the monomer chemical for polymerization of high molecular weight polylactic acid (PLA). The synthesis of cyclic l-lactide starts from poly-condensation of l-lactic acid to a low molecular weight prepolymer and then depolymerized to cyclic l-lactide. Lignocellulose biomass is the most promising carbohydrate feedstock for lactic acid production, but the synthesis of cyclic l
High-average power Ytterbium (Yb) laser systems are playing an increasingly important role in ultrafast science e.g. as pump lasers for optical parametric amplifiers or directly as ultrafast sources. The gain bandwidth of Yb limits the pulse duration to a few 100 fs up to about 1 ps. However, many applications, such as attosecond physics or X-ray Free Electron Laser (FEL) science, would greatly be
The hepatokine follistatin is elevated in patients with type 2 diabetes (T2D) and promotes hyperglycemia in mice. Here we explore the relationship of plasma follistatin levels with incident T2D and mechanisms involved. Adjusted hazard ratio (HR) per standard deviation (SD) increase in follistatin levels for T2D is 1.24 (CI: 1.04-1.47, p < 0.05) during 19-year follow-up (n = 4060, Sweden); and 1.31
Chaperones protect other proteins against misfolding and aggregation, a key requirement for maintaining biological function. Experimental observations of changes in solubility of amyloid proteins in the presence of certain chaperones are discussed here in terms of thermodynamic driving forces. We outline how chaperones can enhance amyloid solubility through the formation of heteromolecular aggrega
Why do citizens’ decisions made because they favour the mitigation of climate change outnumber those made because they favour adaptation to its impacts? Using data collected in a survey of 338 citizens of Malmö, Sweden, we tested two hypotheses. H1: the motivation for personal decisions because they favour adaptation to the impacts of climate change correlates with the decision-making agent´s know
While a broad spectrum of technologies is integrated in everyday life and routines, most research on ageing, health and technology has focused on attitudes toward and adoption of digital technologies including e-health, or home based monitoring systems. The aim of this study was to explore differences and similarities in attitudes and experiences with different types of technologies and developmen
Characterization of directed differentiation of pluripotent stem cells towards therapeutically relevant cell types, including pancreatic beta-cells and hepatocytes, depends on molecular markers and assays that resolve the signature of individual cells. Pancreas and liver both have a common origin of anterior definitive endoderm (DE). Here, we differentiated human embryonic stem cells towards DE us
Background Patients with acute and chronic pain have a significantly reduced quality of life, and it is a clinical impression thatunhealthy lifestyle influences the experience of pain. To evaluate this, surgery can be seen as a controlled trauma and acutepain model with standardised guidelines. The aim was to investigate the association between lifestyle risk factors and experience of heavy postop
Three metabolite patterns have previously shown prospective inverse associations with the risk of aggressive prostate cancer within the European Prospective Investigation into Cancer and Nutrition (EPIC). Here, we investigated dietary and lifestyle correlates of these three prostate cancer-related metabolite patterns, which included: 64 phosphatidylcholines and three hydroxysphingomyelins (Pattern
Cultivated oat (Avena sativa L.) is an allohexaploid (AACCDD, 2n = 6x = 42) thought to have been domesticated more than 3,000 years ago while growing as a weed in wheat, emmer and barley fields in Anatolia1,2. Oat has a low carbon footprint, substantial health benefits and the potential to replace animal-based food products. However, the lack of a fully annotated reference genome has hampered effo
