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Three major cellulases, cellobiohydrolase I and II (CBH I and II) and endoglucanase II (EG II) of Trichoderma reesei have been purified by ion-exchange chromatography in an FPLC system. Microcrystalline cellulose (Avicel) was hydrolysed by the single enzymes and by equimolar mixtures of CBH I-CBH II and CBH I-EG II. Enzyme adsorption was followed indirectly by selectively quantifying the en
