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Intraventricular Infusion of TrkB-Fc Fusion Protein Promotes Ischemia-Induced Neurogenesis in Adult Rat Dentate Gyrus.

Background and Purpose-We have previously shown that delivery of brain-derived neurotrophic factor (BDNF) through direct intrahippocampal gene transduction with a viral vector suppresses the formation of new dentate granule cells triggered by global forebrain ischemia. Here, we investigated whether inhibition of endogenous BDNF alters ischemia-induced neurogenesis in the dentate gyrus. Methods-Rat

Vehicle driving cycle simulation of a pneumatic hybrid bus based on experimental engine measurements

In the study presented in this paper, a vehicle driving cycle simulation of the pneumatic hybrid has been conducted. The pneumatic hybrid powertrain has been modeled in GT-Power and validated against experimental data. The GT-Power engine model has been linked with a MATLAB/simulink vehicle model. The engine in question is a single-cylinder Scania D12 diesel engine, which has been converted to wor

Pool boiling heat transfer of FC-72 on pin-fin silicon surfaces with nanoparticle deposition

In the present study, two types of micro-pin–fin configurations were fabricated on silicon surfaces by a dry etching method, i.e., staggered pin fins (#1) and aligned pin fins with empty areas (#2). The micro-pin–fin surfaces were then further modified by depositing FeMn oxide nanoparticles (∼35 nm) electrostatically for 8 h and 16 h, respectively, namely #1-8h, #1-16h, #2-8h and #2-16h. Subcooled

EndoS and EndoS2 hydrolyze Fc-glycans on therapeutic antibodies with different glycoform selectivity and can be used for rapid quantification of high-mannose glycans.

Enzymes that affect glycoproteins of the human immune system, and thereby modulate defense responses, are abundant among bacterial pathogens. Two endoglycosidases from the human pathogen Streptococcus pyogenes, EndoS and EndoS2, have recently been shown to hydrolyze N-linked glycans of human IgG. However, detailed characterization and comparison of the hydrolyzing activities have not been performe