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A targeted metabolomic protocol for quantitative analysis of volatile organic compounds in urine of children with celiac disease

Volatile organic compounds (VOCs) in biological samples have gained popularity for disease monitoring and diagnosis. Celiac disease (CD) is one of the many prevalent health conditions which are challenging to diagnose. The aim of this study was to optimize a solid phase microextraction followed by gas chromatography-mass spectrometry, for quantitative analysis of a wide range of VOCs in the urine

Determination of reducing ends with flow injection analysis with amperometric detection: application to enzyme-hydrolysed methyl cellulose

A novel method for detection of reducing ends of sugars is proposed, based on the use of Fe(CN)(6)(3-) as the oxidant in combination with amperometric detection and flow injection analysis (FIA). The method is very sensitive, giving values of < 10 mu M for the limit of detection for a series of mono- and oligosaccharides. Samples can be analysed every 30 s, and injection can be made fully automate

The draft genome of whitefly Bemisia tabaci MEAM1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance

Background: The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is among the 100 worst invasive species in the world. As one of the most important crop pests and virus vectors, B. tabaci causes substantial crop losses and poses a serious threat to global food security. Results: We report the 615-Mb high-quality genome sequence of B. tabaci Middle East-Asia Minor 1 (MEAM1), the first genome sequen

Finite Element Analysis of Structure-acoustic Systems : Methods and Applications

Paper 1The problem in which a flexible structure interacts with an acoustic fluid is analysed by use of the finite element method. With increasing complexity of the geometry and when increasing the frequency limit is of interest, the number of degrees of freedom needed to describe the system becomes very large. To reduce the coupled system, modal analysis is performed in the structural and in the

Ant13 Encodes Regulatory Factor WD40 Controlling Anthocyanin and Proanthocyanidin Synthesis in Barley (Hordeum vulgare L.)

Flavonoid compounds like anthocyanins and proanthocyanidins are important plant secondary metabolites having wide biological activities for humans. In this study, the molecular function of the Ant13 locus, which is one of the key loci governing flavonoid synthesis in barley, was determined. It was found that Ant13 encodes a WD40-type regulatory protein, which is required for transcriptional activa

HPV self-sampling versus healthcare provider collection on the effect of cervical cancer screening uptake and costs in LMIC : a systematic review and meta-analysis

Background: Cervical cancer is a major global health issue, with 89% of cases occurring in low- and middle-income countries (LMICs). Human papillomavirus (HPV) self-sampling tests have been suggested as an innovative way to improve cervical cancer screening uptake and reduce the burden of disease. The objective of this review was to examine the effect of HPV self-sampling on screening uptake compa

Fracture Characterization of Wood Adhesive Joints

Failure in wood is commonly considered as a sign of properly chosen adhesive for wood adhesive joints. It is argued that as the adhesive is stronger than the surrounding wood material, no concern has to be given to the adhesive properties in joint design. However, by means of theoretical arguments in combination with experimental experience in the present study, it is stated that local bond line p

DOSIMETRY AND CANCER RISK ESTIMATIONS FOR DIFFERENT RADIATION PROTECTION SOLUTIONS AT DECOMMISSIONING A CONTAMINATED NUCLEAR POWER PLANT SITE

Contaminated sediments originating from dredging activities in a nuclear power plant site were placed in a pond, which has to be taken into consideration during the future decommissioning process. The sediments have to be handled to free release the site. The radionuclides Co-60 and Cs-137 were identified and the activity concentrations (Bq/kg) were quantified in the range of 10-6000 and 5-50 Bq k

Super-Earth of 8 M in a 2.2-day orbit around the K5V star K2-216

Context. Although thousands of exoplanets have been discovered to date, far fewer have been fully characterised, in particular super-Earths. The KESPRINT consortium identified K2-216 as a planetary candidate host star in the K2 space mission Campaign 8 field with a transiting super-Earth. The planet has recently been validated as well. Aims. Our aim was to confirm the detection and derive the main

Non-corticosteroid adjuvant therapies for acute bacterial meningitis

Background: Acute bacterial meningitis is a bacterial infection of the membranes that surround and protect the brain, known as the meninges. The primary therapy for bacterial meningitis is antibiotics and corticosteroids. Although these therapies significantly improve outcomes, bacterial meningitis still has a high risk of death and a high risk of neurological sequelae in survivors. New adjuvant t

Linguistic landscaping in Singapore : multilingualism or the dominance of English and its dual identity in the local linguistic ecology?

Although Singapore, a linguistically and ethnically diverse city–state, uses four official languages, namely Malay, Mandarin, Tamil, and English, which are supposed to enjoy equal status, there appears to be a pecking order to these languages. English seems to be the dominant language when taking into consideration the bilingual education policy, numerous campaigns promoting its use in terms of sp

phenopype : A phenotyping pipeline for Python

Digital images are an intuitive way to capture, store and analyse organismal phenotypes. Many biologists are taking images to collect high-dimensional phenotypic information from specimens to investigate complex ecological, evolutionary and developmental phenomena, such as relationships between trait diversity and ecosystem function, multivariate natural selection or developmental plasticity. As a

Gaia Early Data Release 3: Structure and properties of the Magellanic Clouds

Context. This work is part of the Gaia Data Processing and Analysis Consortium papers published with the Gaia Early Data Release 3 (EDR3). It is one of the demonstration papers aiming to highlight the improvements and quality of the newly published data by applying them to a scientific case.Aims. We use the Gaia EDR3 data to study the structure and kinematics of the Magellanic Clouds. The large di

Improving emergency and disaster response management performance : A problem-solving perspective

The purpose of this doctoral thesis is to investigate how collective emergency and disaster response management performance can be further improved. Based on four studies, this research contributes with knowledge in two areas.First, collective processes that might improve response management are investigated. In this regard, collective improvisation as a concept is found limited in its usefulness

Comparison of posterior hyaloid assessment using preoperative optical coherence tomography and intraoperative triamcinolone acetonide staining during vitrectomy

Purpose: To compare the specificity of diagnosing posterior vitreous detachment (PVD) using preoperative optical coherence tomography (OCT) versus intraoperative triamcinolone acetonide (TA) staining in patients undergoing vitrectomy. Patients and Methods: This retrospective cohort study included patients undergoing pars plana vitrectomy for diverse retinal pathologies. Intraoperatively, surgeons

Isolation and characterization of a tumor necrosis factor binding protein from urine

Tumor necrosis factor (TNF)/cachectin can produce both beneficial and harmful manifestations. Mechanisms may operate to counteract potentially harmful effects such as shock and cachexia. The TNF binding protein (TNF-BP), which is found at increased levels in serum and urine of patients with chronic renal failure, may play such a role. TNF-BP was purified 1,000,000-fold to homogeneity from urine of

Tender points as predictors of distress and the pharmacologic management of fibromyalgia syndrome

The object of this study was to determine the association between tender point pain ratings, tender point counts and distress in people with fibromyalgia and to review the pharmacotherapy of fibromyalgia. Demographic, psychosocial, and health status information was collected from 316 health maintenance organization members with fibromyalgia. A manual tender point exam was conducted. Tender point c