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Sensitive detection of molecular biomarkers in clinical samples is crucially important in disease diagnostics. This paper reports the developement of an aptamer microarray platform combined with sol-gel technology to identify low-abundance targets in complex serum samples. Because of the nanoporous structure of the sol-gel, a high capacity to immobilize the affinity specific aptamers is accomplish
INTRODUCTION AND MATERIALS AND METHODS: G-protein coupled receptors (GPCRs) play an important role in platelet aggregation. To identify new platelet GPCRs, a platelet gene expression profile was generated and validated using quantitative real-time PCR. RESULTS: In total, mRNA of 28 GPCR genes was detected in human platelets. The 12 most abundant platelet GPCR transcripts were: thrombin receptor PA
We present palaeomagnetic and cosmogenic radionuclide records of the Laschamp geomagnetic excursion in Lake Pupuke, a maar lake in Auckland, New Zealand. Laschamp was identified by a combination of relative palaeointensity, (10)Be and (14)C data from the lake sediments and represents the first such record from the Southern Hemisphere. Despite the high organic carbon content, which causes relativel
Saccharomyces cerevisiae was exposed to inhibitory concentrations of the three phenolic phenylpropanoids: coniferyl aldehyde, ferulic acid, and isoeugenol. Deoxyribonucleic acid microarray analysis was employed as one approach to generate a set of candidate genes for deletion mutant analysis to determine the potential contribution of the corresponding gene products to the resistance against toxic
Bildandet av blodceller, hematopoesen, utgår från ett litet antal av de så kallade hematopoetiska stamceller som sitter i vår benmärg. Dessa celler har förmågan att ge upphov till alla mogna blodcellstyper av de tre hematopoetiska linjerna, samtidigt som de genom celldelning bibehåller sin egna mängd. Det har uppskattats att vi endast har några tusen hematopoetiska stamceller, men trots deras rela
The neutron-deficient gold (Z=79) isotopes in the vicinity of the neutron midshell N=104 provide prolific examples of shape coexistence and isomerism at low excitation energy. They can be probed via a number of different experimental techniques. In this study, two new isomeric states with half-lives of 294(7) and 373(9) ns have been observed in the neutron-deficient odd-odd nuclide Au178 (N=99) in
Folding properties of a two-dimensional toy protein model containing only two amino acid types, hydrophobic and hydrophilic, respectively, are analyzed. An efficient Monte Carlo procedure is employed to ensure that the ground states are found. The thermodynamic properties are found to be strongly sequence dependent in contrast to the kinetic ones. Hence, criteria for good folders are defined entir
In connection to an environmental investigation of a former industrial site, the cretaceous sandy limestone bedrock in the surrounding area were mapped with resistivity and time domain induced polarization (DCIP). The aim of the DCIP survey was to delineate geological units and possible preferential pathways of DNAPL contaminated groundwater. Although IP anomalies corresponding to fractured crysta
Four recombinant Saccharomyces cerevisiae strains with enhanced xylose growth (TMB3400, C1, C5 and BH42) were compared with two control strains (TMB3399, TMB3001) through genome-wide transcription analysis in order to identify novel targets for inverse metabolic engineering. A subset of 13 genes with changed expression levels in all improved strains was selected for further analysis. Thirteen vali
Pseudogenes, nonfunctional copies of genes, evolve fast due the lack of evolutionary pressures and thus appear in several different forms. PseudoGeneQuest is an online tool to search the human genome for a given query sequence and to identify different types of pseudogenes as well as novel genes and gene fragments.
Phenolic compounds, abundant in plants, are of significant interest and are increasingly becoming a subject of intensive research due to their bioactive properties such as antioxidant activity. The objective of this study was to identify phenolic compounds present in formulated quinoa milk fermented by two probiotic bacteria strains; Lactobacillus pentosus and Pediococcus pentosaceous by HPLC and
Arabinoxylan (AX) is a dietary fibre linked to positive health effects. An oat mutant population was screened for AX content and oat lines in the range 3,63-12,4% AX and a mean of 7.5% were identified. The mean of reference oat variety Belinda was 8,05% AX. 15 potential oat AX biosynthetic genes from four different genes families (UAM, GT43, GT47, GT61) were identified. A selection of six genes id
There are hundreds of thousands of moth species with crucial ecological roles that are often obscured by their nocturnal lifestyles. The pigmentation and appearance of moths are dominated by cryptic diffuse shades of brown. In this study, 82 specimens representing 26 moth species were analysed using infrared polarimetric hyperspectral imaging in the range of 0.95–2.5 µm. Contrary to previous studi
A procedure to identify the imperfection in thin plates is proposed in this paper. The modified potential energy principle, which serves as the theoretical basis of the identification procedure, is improved to allow for the experimental measurements in static tests. Several typical examples are studied to illustrate the effectiveness of the procedure.
The characterization of the incident photon beam is usually divided into its dependence on collimator setting (head-scatter factor) and off-axis position (primary off-axis ratio). These parameters are normally measured "in air" with a build-up cap thick enough to generate full dose build-up at the depth of dose maximum. In order to prevent any influence from contaminating electrons, it has been re
The characterization of the incident photon beam is usually divided into its dependence on collimator setting (head-scatter factor) and off-axis position (primary off-axis ratio). These parameters are normally measured "in air" with a build-up cap, thick enough to generate full dose build-up at the depth of dose maximum. In order to prevent any influence from contaminating electrons, it
Birth defects, which are in part caused by exposure to environmental chemicals and pharmaceutical drugs, affect 1 in every 33 babies born in the United States each year. The current standard to screen drugs that affect embryonic development is based on prenatal animal testing; however, this approach yields low-throughput and limited mechanistic information regarding the biological pathways and pot
Access to clean and affordable energy is one of the cornerstones of the world's society. Since the introduction of the internal combustion engine, diesel engines have become widely used for transportation in the commercial sector. These engines are attractive as they have low fuel consumption, but are also associated with high emissions of air pollutants, such as NO$_X$ and soot. These emissions a
