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A Yersinia PCR-Compatible Enrichment (YPCE) medium was developed, which removes the necessity for sample pretreatment before PCR-based detection of Yersinia enterocolitica. The medium was designed through a sequence of independent screening and factorial design experiments to study the PCR inhibition and growth characteristics of medium components. The compatibility of the YPCE medium was evaluate
We have employed Lu-Hf apatite chronology to determine the age of a magnetite-rich melatroctolite in southern Sweden, known as the Smalands Taberg Fe-Ti ore deposit. The melatroctolite is poor in incompatible elements and minerals forming at more advanced stages of differentiation, which precludes the use of minerals (baddeleyite, zircon, titanite or apatite) commonly used for dating the emplaceme
The ultrastructural cytology and reproduction of the hyperparasitic microsporidium Amphiamblys copitellides (Caullery and Mesnil, 1897) is described. Merogonial reproduction was not observed. The sporogony comprises two sequences: a sac-bound sporogony in close contact with the cytoplasm of the host and a free sporogony in parasitophorous vacuoles. The free sporogony, which probably precedes the s
We report the first experimental measurements and theoretical calculations for the hyperfine splitting of the 3d(5) 4s ground state of Mn II. The hyperfine structure constants were determined using Fourier transform spectroscopy with a hollow cathode discharge lamp. The uncertainties in the magnetic dipole constants, A, are between 3 x 10(-4) and 5 x 10(-4) cm(-1). Extensive configuration interact
In this study, we assessed the proliferative response of peripheral blood mononuclear leukocytes (PBML) from 33 children/young adolescents with chronic dyspepsia, to H. pylori LPS in the presence and absence of IL-2 as a T cell growth factor. A rapid urease test (RUT) and a presence of Helicobacter-like organisms (HLO) in the biopsy specimens allowed us to distinguish RUT/HLO-positive (17/33) and
Sucrose synthase (SuSy) is a key enzyme in the development of storage root of radish. Clarification of its spatial and temporal expression during the thickening growth of radish hypocotyl, which later develops into storage root, was carried out immunologically using light microscopy. Sequential harvests at 3, 7, 11 and 13 d after sowing (DAS) were performed on two radish cultivars having different
The microarray technology has revolutionized biological research in the last decade. By monitoring the expression of many genes simultaneously, microarrays can elucidate gene function, as well as scan entire genomes for candidate genes encoding complex traits. However, because of high costs of sequencing and design, microarrays have largely been restricted to a few model species. Cross-species mic
Removal of nitrite and nitrate from drinking water has attracted great attention in recent years because of the human health risk induced by the exposure to contaminated groundwater and surface water. We have therefore tested a model nitrite oxidation system by coentrapping the NaNO2 oxidizer Nitrobacter vulgaris with polydimethylsiloxane (PDMS) copolymer and DEAE-Sephadex in a polyacrylamide gel.
Floral scent has been suggested to play a key role in the obligate pollination mutualism between yuccas and yucca moths. We analyzed floral fragrance compounds of Yucca elata with headspace collection followed by gas chromatography and mass spectrometry, and compared the odor blend with the recently characterized blend of the allopatric Yucca filamentosa. A principal component analysis based on 20
In preterm infants, low levels of Insulin like growth factor 1 (IGF-I) have been associated with impaired growth and retinopathy of prematurity. Our objective was to study safety and pharmacokinetics of intravenously administered rhIGF-I with its binding protein 3 (rhIGFBP-3) to preterm infants. At 3 days chronological age, an intravenous 3 hours infusion of rhIGF-I/rhIGFBP-3 was administered foll
It is well established that physical inactivity in adults is associated with increased mortality and morbidity. Whether daily physical activity level is related to risk factors for cardiovascular disease (CVD) in children has been debated. Furthermore, objective data on the habitual daily physical activity in children have at large been scarce in the literature. The main reason for this is the fac
We have previously shown that ghrelin is mainly localized to the stomach but also occurs, together with the prokinetic hormone motilin, in endocrine cells in the proximal small intestine. This study explored ghrelin and motilin concentrations in plasma in relation to gastrointestinal motility and whether plasma ghrelin is changed in patients with irritable bowel syndrome (IBS). Nine patients with
There is currently a challenge to produce an electrocardiogram (ECG) recording of waveforms that are "standard" and also achieve the "noise immunity" required for continuous monitoring. The potential solutions that have been considered are to label each torso-recorded ECG as "nonstandard, torso-recorded," designate torso placement as "standard," or to reconstruct the "equivalent distally recorded
The PR interval on the electrocardiogram reflects atrial and atrioventricular nodal conduction time. The PR interval is heritable, provides important information about arrhythmia risk, and has been suggested to differ among human races. Genome-wide association (GWA) studies have identified common genetic determinants of the PR interval in individuals of European and Asian ancestry, but there is a
Bacterial skin infections, such as erysipelas or cellulitis, are characterized by fever and a painful erythematous rash. Despite the high prevalence of these infections, little is known about the underlying pathogenic mechanisms. This is partly due to the fact that a bacterial diagnosis is often difficult to attain. To gain insight into the pathogenesis of erysipelas, we investigated the samples o
