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Computer programming should be expressing the complicated in easily understandable parts. General languages provide tools and abstractions that allow many different problems to be formulated and solved. Unfortunately, these abstraction only rarely match the problem area precisely, resulting in solutions that are obscured by the need for support code unrelated to the problem. This can be avoided, b
A new type of fibre-based anion-exchange material for plasmid purification was developed. The basic material consisted of non-porous silica fibres with a mean diameter of 1.5 mu m and a surface area of 2.4 m(2) g(-1). The fibre surface was provided with several types of ligands, either by adsorption of polymers (chitosan or poly(ethyleneinune)) or by polymerization of amine-containing acrylic mono
Polychlorinated biphenyls (PCBs) in water were extracted with a rebuilt extraction unit using 47 mm C18 solid-phase extraction (SPE) disks. Three types of disks (SPEC, ENVI and Empore) were investigated for the extraction of seven PCBs from 1 l reagent water spiked at two concentration levels (20 and 1000 ng/l). The Empore disks produced the best analyte recoveries (91–107% with R.S.D. of 1–8%) at
Popular AbstractSveriges relativa välstånd kan vi till stor del tacka skogen för. Skogen har ända sedan industrialiseringen började ta fart i Sverige bidragit med betydande exportinkomster. För att bevara den inkomstkälla skogen utgör måste vi se till att det skogsbruk som bedrivs är uthålligt och tar hänsyn till såväl ekonomiska som biologiska och sociala aspekter. Skogsproduktionen ska med andraIt took 70-80 years to realize the width of the impact of industrialization on the forest ecosystems since a forest ecosystem is a "slow system" as seen from a human perspective. An anticipated future climate change and increasing carbon dioxide and ozone levels will change the conditions even more with time and the widely used statistical forest growth models will not be applicable anymore. Compu
Nuclear quadrupole resonance (NQR) offers an unequivocal method of detecting and identifying land mines. Unfortunately, the practical use of NQR is restricted by the low signal-to-noise ratio (SNR), and the means to improve the SNR are vital to enable a rapid, reliable, and convenient system. In this paper, an approximate maximum-likelihood detector (AML) is developed, exploiting the temperature d
The primary purification of a 6.1kilo base pair (kbp) plasmid from a desalted alkaline lysate has been accomplished by a thermoseparating aqueous two-phase system [(50% ethylene oxide-50% propylene oxide)-Dextran T 500]. The partitioning of the different nucleic acids (plasmid DNA, RNA, genomic DNA) in the thermoseparating aqueous two-phase system was followed both qualitatively by agarose gel ele
Inhibition of cell growth is the most commonly used endpoint for in vitro toxicity of biomaterials. The use of several different endpoints might however generate more information concerning the nature of the toxicity. Thus, we examined the toxicity of two biomaterials, Polyvinylchloride (PVC) and Polyoximethene (POM), with different selected endpoints. The influence of cell growth on these endpoin
The phase behavior of a thermoseparating cationic hydrophobically modified ethylene oxide polymer (HM-EO) containing tertiary amines has been investigated at different pH, salt and sodium dodecyl sulfate (SDS) concentrations, in order to find a water/HM-EO two-phase, system suitable for protein partitioning. The used polymer forms micellar aggregates that can be charged. By changing pH and SDS con
This paper presents findings from two explorative studies. One is a consumer study based on interviews with consumers in real store settings and one is a study based on interviews with fruit and vegetable managers of stores affiliated with a major food retail chain in Sweden. The aim of both studies was to explore the role of the grocery store for the consumers' choice of organic versus convention
Objectives: The aim of this survey was to compare stressful life events, social support and sense of coherence (SOC) between frequent attenders (FAs) and normal attenders (controls) in primary health care. Study design: A cross-sectional study was conducted in a primary healthcare centre in the south-west of Sweden. Methods: A postal questionnaire was sent to 263 frequent attenders and 703 normal
We present further properties of a previously proposed recursive scheme for parametrization of n-by-n unitary matrices. We show that the factors in the recursive formula may be introduced in any desired order. The method is used to study the invariant phases of unitary matrices. The case of four-by-four unitary matrices is investigated in detail. We also address the question of how to construct sy
A preliminary systematic position of the genus Cetrariella based on sequences from the ITS region is discussed. Cetrariella delisei and C. fastigiata appear as not very closely related species. Both species form a monophyletic sister group to Cetraria and should presumably stay as a separate segregate. The evidences from morphological data and data from secondary chemistry, however, strongly suppo
Mathematical and numerical modeling of the complex interplay between compaction, flow, and hydrodynamic dispersion in a chromatographic column under steady stale conditions has been examined. The mathematical model was based on pure elastic analysis and Darcy flow. A two-dimensional control volume approach was applied to solve the coupled nonlinear equations describing the flow and compaction. The
We have investigated pre-travel advice, behaviour, chemoprophylaxis and diagnostic delay in travellers returning to Sweden with falciparum malaria. Questionnaires were distributed to patients having been notified with falciparum malaria from 1994 to 2001. Of 408 notified patients, 237 (58%) returned the questionnaires; 62% were males and 43% above the age of 45 y. Africa was the travel destination
A new approach in affinity separations was studied by partitioning of Cellulomonas fimi beta-mannanase (EC 3.2.1.78) containing a mannan-binding module in galactomannan/hydroxypropyl starch aqueous two-phase system. Comparison was made with a truncated version of C. fimi beta-mannanase which lacked the mannan-binding module. Results showed that affinity partitioning of the beta-mannanase was achie
Women in the 19th century in the south of Sweden. Lifestyle, gender relations, women's emancipation
To reveal the meaning of being a nurse working with inpatient care on a team psychiatric ward in Sweden, 22 psychiatric nurses were interviewed and the transcribed texts were analysed by means of latent content analysis. Three themes emerged from the analysis: developing a working relationship with the patient in everyday caregiving; encountering and handling the unforseeable in daily living; and
Bacteriuria is associated with acute disease conditions of varying severity.48 and 62 Consequently, the definition of bacterial virulence for the urinary tract depends on the end-point chosen. Acute pyelonephritis is characterized by fever, flank pain, and general malaise. Laboratory findings include pyuria (leukocytes in the urine), elevated acute phase reactants in serum (C reactive protein [CRP
