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In this study, the growth of a short edge crack during more than 14 000 cycles of fatigue loading is investigated in detail. An edge crack, in a semi-infinite body with no pre-existing obstacles present, is modelled in a boundary element approach by a distribution of dislocation dipoles. The fatigue cycles are fully reversed (R = -1), and the load range is well below the threshold for long fatigue
A dot blot hybridization method was developed to detect Plasmodium falciparum sporozoites in naturally infected mosquitoes. A fluorescein-labelled oligomer was used as a probe. Initial non-specific hybridization was found to correlate with the presence of blood in the mosquitoes. This was eliminated by allowing digestion of the engorged blood by keeping the mosquitoes in cages for 48 h before proc
Mercury is a severe pollutant in China. A novel mobile laser spectroscopy laboratory was constructed and used in differential absorption lidar mapping in major Chinese cities, a heavily polluted mining area and an archeological site.
Enhancing stability of YAC in S. cerevisiae The aim of the study was to develop S. cerevisiae strain that can maintain yeast artificial chromosome (YAC) for prolonged period. The enzymes for taxadiene biosynthesis were cloned on YAC and introduced into engineered strain. Ultimately heterologous pathway was superimposed on native ergosterol pathway. The strain development was achieved by target
An experimental study to characterize properties controlling delamination of paperboard is presented. The normal and shear traction–separation laws are measured and evaluated using a double cantilever beam (DCB) and a split double cantilever beam (SCB) specimen. The DCB-experiments provides normal separation data in good agreement with results using alternative experimental techniques. From the me
Bacterial proteases are important enzymes used in several technical applications where controlled cleavage of proteins is needed. They are challenging enzymes to express recombinantly as parts of the proteome can be hydrolyzed by their activity. The eukaryotic model organism Saccharomyces cerevisiae is potentially a good expression host as it tolerates several stress conditions and is known to bet
A multiple GnRHa injection method for inducing spawning of high fecundity and quality in meagre was optimized. Specifically, the study (a) examined how long females would continue spawning in response to consecutive, weekly GnRHa injections, if maintained at the natural spawning temperature, and (b) evaluated whether males can produce adequate amounts of good quality sperm for the same period, wit
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NOx pollution from diesel engines has been stated as causing over 10 000 pre-mature deaths annually and predictions are showing that this level will increase [1]. In order to decrease this growing global problem, exhaust after-treatment systems for diesel engines have to be improved, this is especially so for vehicles carrying freight as their use of diesel engines is expected to carry on into the
The hindered diffusion and binding of proteins of different sizes (lysozyme, BSA and IgG) in an agarose gel is described using adsorption kinetic and diffusional data together with an experimentally determined pore size distribution in the gel. The validity of the pore model, including variable diffusion coefficients and porosities is tested against experimental confocal microscopy data. No fittin
The accurate and reliable detection of opening of doors and windows is vital for home security applications. This master thesis aims to present a way to achieve this using a low-cost and low-power ecompass, containing a MEMS accelerometer and a magnetometer. This has been achieved by attaching such a device to a door and collecting sensor data when opening and closing the door. Said data were then
Haemophilus influenzae type b (Hib) is, in contrast to non-b H. influenzae, associated with severe invasive disease such as meningitis and epiglottitis in small children. To date accurate H. influenzae capsule typing requires PCR, a time-consuming and cumbersome method. MALDI-TOF MS provides rapid bacterial diagnostics and is increasingly used in clinical microbiology laboratories. Here MALDI-TOF
The biochemistry and molecular genetics underlying the related carbohydrate blood group antigens P, P(k), and LKE in the GLOB collection and P1 in the P blood group system are complex and not fully understood. Individuals with the rare but clinically important erythrocyte phenotypes P(1)k and P(2)k lack the capability to synthesize P antigen identified as globoside, the cellular receptor for Parvo
Applications of system identification techniques to chemical, metallurgical, paper and pulp, cement and glass industries are surveyed. It is demonstrated that identification is a useful tool to determine process dynamics. This is true, particularly for processes which are difficult to model from basic physical laws only, such as blast furnaces, paper machines and cement kilns. Valuable knowledge i
Spin-echo modulation small-angle neutron scattering (SEMSANS) is based on the detection of spatial beam modulation, which is induced by triangular spin echo precession regions and subsequent spin analyses. In order to detect such signal and exploit it for small angle scattering investigations neutron detection with sub-millimeter spatial resolution is required. Here an approach is reported where i
Motivation: The precise prediction of protein domains, which are the structural, functional and evolutionary units of proteins, has been a research focus in recent years. Although many methods have been presented for predicting protein domains and boundaries, the accuracy of predictions could be improved. Results: In this study we present a novel approach, DomHR, which is an accurate predictor of
