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Abstract in Spanish Antecedentes: Las pruebas cutáneas con determinantes mayores y menores de la penicilina se utilizan de forma habitual para el diagnóstico de reacciones de hipersensibilidad inmediata a los antibióticos betalactámicos. Estos extractos diagnósticos han sido recientemente retirados del mercado europeo. Objetivo: Estudio de una nueva marca de determinantes para el diagnóstico de reBACKGROUND: Skin testing with major and minor determinants of benzylpenicillin is recommended standard practice for the evaluation of patients with immediate hypersensitivity reactions to beta-lactams. However, commercial reagents for this purpose were recently dropped from the European market. OBJECTIVE: In the present study, we assessed a new brand of reagents for use in skin testing in patients

Many intimate associations between different species of ectomycorrhizal fungi are inferred on the basis of the consistent cooccurrence of their fruit bodies. Suillus bovinus and Gomphidius roseus, where the latter never occurs without the former, is one example. This association was examined with PCR identification and light microscopy. S. bovinus and G. roseus were unambiguously separated on the

Objective: To develop a short measure of physical function for knee osteoarthritis (OA) using multi-national data from. individuals with varying degrees of severity of knee OA. Methods: Rasch analysis, based on the partial credit model, was conducted on Knee injury and Osteoarthritis Outcome Score and Western Ontario McMaster Universities' Osteoarthritis Index data from individuals with knee OA, r

The performance of a miniaturized sample processing platform for matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), manufactured by silicon microfabrication, called integrated selective enrichment target (ISET) technology was evaluated in a biological context. The ISET serves as both sample treatment device and MALDI-MS target, and contains an array of 96 perforated nanovial

Aim/hypothesis Hyperinsulinaemia maintains euglycaemia in insulin-resistant states. The precise cellular mechanisms by which the beta cells adapt are still unresolved. A peripherally derived cue, such as increased circulating fatty acids, may instruct the beta cell to initiate an adaptive programme to maintain glucose homeostasis. When this fails, type 2 diabetes ensues. Because mitochondria play

Methods for facile synthesis of symmetric and unsymmetric functionalized analogues of Tröger's base were developed with use of 2,8-dibromo-6H,12H-5,11-methanodibenzo[b,f][1,5]diazocine (2) as the starting material. C(2)-symmetric 2,8-disubstituted analogues of Tröger's base (4a-f) were synthesized via double bromine-lithium exchange of 2 followed by quench with electrophiles. Desymmetrization via

Purpose – This paper aims to contribute to the tactical and operational decision making of manufacturing and logistics operations by providing novel insights into modelling and simulation, based on complex adaptive systems (CAS). Design/methodology/approach – The research approach is theoretically based on CAS with agent-based modelling (ABM) as the implementation method. A case study is presented

Forty-three strains of group B streptococci (GBS) of types Ia, Ib, II and III were tested for susceptibility to chlorhexidine in concentrations ranging from 256 to 0.25 mg/l using the agar and tube dilution methods. The strains showed minimum inhibitory concentration (MIC) values ranging from 0.5 to 1 mg/l. Serum added to the test medium (50%) increased the MIC values to 4-8 mg/l, while amniotic f

Over 70 equations approximating thermodynamic and transport properties of water and steam at pressure 1 bar and saturated pressure as well as air at pressure 1 bar were tested against reference data. Their accuracy and relative computing time were reported.

OBJECTIVES: To standardise the colorimetric assay based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) for the rapid detection of rifampicin-resistant Mycobacterium tuberculosis in clinical practice and to evaluate the assay on a collection of 92 clinical isolates. DESIGN: The Bactec method was used as the reference method. Rifampicin was used for the susceptibility testing

We used roentgen stereophotogrammetric analysis to follow 33 C-stem femoral components for two years after primary total hip arthroplasty. All components migrated distally and posteriorly within the cement mantle. The mean distal migration was 1.35 mm (SD 0.62) at two years and the mean posterior migration was 1.35 mm (SD 0.69) at two years. All the femoral components rotated into retroversion wit

Ion microprobe U-Th-Pb analyses of zircons in variably metamorphosed and veined orthogneisses in the southern part of the parautochthonous Eastern Segment of the Sveconorwegian (1.20-0.90 Ga) orogen, SW Sweden, broadly define two age groups, oscillatory and sector zoned magmatic zircon cores yield 1.70-1.68 Ga while overgrowths, homogeneous crystals, and recrystallized domains in primary zircon yi

OBJECTIVES: The aim of this study was to identify clinical characteristics of depression in elderly people that could be useful for case finding in general practice. DESIGN: A cross-sectional study of clinical characteristics through review of medical records. SETTING: Herrhagen health centre, Karlstad, Sweden. SUBJECTS: Seventy-one persons with a high depressive score in a screening of depressive

The Bacillus subtilis aprE leader is a determinant of extreme mRNA stability. The authors examined what properties of the aprE leader confer stability on an mRNA. The secondary structure of the aprE leader mRNA was analysed in vitro and in vivo, and mutations were introduced into different domains of an aprE leader-lacZ fusion. The half-lives of the corresponding transcripts were determined and be

Purpose: To assess the psychometric properties of a Swedish version of the Posttraumatic Diagnostic Scale (PDS); to investigate the prevalence of traumatic experiences, trauma types and posttraumatic stress disorder (PTSD) in a sample of patients seeking treatment for chronic pain; and to examine how indices of pain-related functioning vary with a history of traumatic exposure and PTSD diagnostic

When developing Community Mental Health Services to support people with psychiatric disabilities, European countries are advocating evidence-based practice (EBP). Individual Placement and Support (IPS) is an evidence-based model designed to support people in acquiring and maintaining competitive employment. Implementation science is a growing research field, with a focus on components that impact