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Objectives: The purpose of this study was to design, validate, and optimize internally standardized real-time quantitative RT-PCR assays and to identify and avoid problems with assay reliability and examine the impact of an exogenous internal standard. Design and methods: The model system consisted of internally standardized quantitative real-time RT-PCR assays specific for PSA and hK2 mRNA based

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Conventional methods often impose difficulties when measuring the viscosity of fluids containing particles or fibres, e.g. tomato paste or fruit preparations, mainly due to the narrow gaps of the viscometer. In order to solve these problems different geometries have been developed, e.g. different mixer systems and wide gap double concentric cylinders. However, these can not be evaluated assuming a

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Optimization of fed-batch conversion of lignocellulosic hydrolyzates by the yeast Saccharomyces cerevisiae was studied. The feed rate was controlled using a step response strategy, in which the carbon dioxide evolution rate was used as input variable. The performance of the control strategy was examined using both an untreated and a detoxified dilute acid hydrolyzate, and the performance was compa

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Gaucher disease (GD), the most common lysosomal storage disorder (LSD), is caused by defects in the activity of the lysosomal enzyme, glucocerebrosidase, resulting in intracellular accumulation of glucosylceramide (GlcCer). Neuronopathic forms, which comprise only a small percent of GD patients, are characterized by neurological impairment and neuronal cell death. Little is known about the pathway

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Purpose: To evaluate long-term patency and clinical efficacy of subintimal angioplasty (SAP) of occluded infrainguinal arteries 3 years post procedure. Methods: One hundred eighty-one patients (92 men; median age 79 years) underwent attempted SAP in 193 limbs with occluded infrainguinal arteries during the period 1999 to 2001. Nearly half (83, 46%) of the patients had diabetes. Most (172, 95%) had

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The removal of chemical oxygen demand (COD) from wastewater-containing phenol was investigated using three-phase three-dimensional electrode reactor. Special attention was paid to experimentally probe the performance of the reactor in COD removal in the process of repeated batch runs. The experimental results showed that the reactor could remove COD from phenol-containing wastewater much more effi

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Title: The impact of environment on music students' commitment to music - a study of attitudes among music students and their parents. The purpose of this essay is to illustrate the importance of environmental influence on childrens' musical interests and factors inspiring children to play instruments and sing. The parents' opinions, regarding the extent of the effect of the environmen

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Denna examensrapport har kommit till som resultat av en varumärkesanalys av bioteknikföretaget Novozymes nya varumärke "Rethink Tomorrow". Syftet med studien har varit att komma fram till hur jag ska presentera varumärket samt den nya produktionen på dotterbolaget Novozymes Biopharma Sweden AB i en stillbildsfilm. Utöver varumärkesanalysen har jag även gjort en kvalitativ enkätstudie där

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This thesis investigates the Designated Operational Entities (DOEs) under the Clean Development Mechanism (CDM) of the Kyoto Protocol. The DOEs serves as independent third-party auditors with the task of validating, verifying and certifying projects under the CDM. The majority of the DOEs are private entities. This is in line with the CDM objective of including the private sector in the climate ch

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Förflyttningshjälpmedel kan skapa svårigheter att ta sig fram i den fysiska miljön, vilket kallas tillgänglighetsproblem. Användbarhetsproblem kan också uppstå, som innebär att personen inte kan göra det han eller hon vill i den fysiska miljön. Lek och rekreation är viktiga aktiviteter för barn. Syftet med denna studie var att få förståelse för vilka platser barn som använder förflyttningshjälpmed

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This thesis is an attempt to discuss the proposals to improve the statuses and rights of the legally resident third country nationals living in the European Union. As denizens, third country nationals have full civil and social rights but they do not have any political rights. This is believed to denote an anomaly, which can be corrected through the grant of political rights. However, political ri

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Offentliga verksamheter genomgår ständigt olika typer av omorganisationer. Sedan 1950-talet har Sveriges förvaltning präglats av omväxlande mer centraliserad styrelseform eller mer decentraliserad styrelseform. Uppsatsen syftar till att undersöka hur regeringar, genom tiderna, argumenterat för omorganisationer av offentliga verksamheter. Förvaltningen är av en särskild karaktär och skall ta hänsyn

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Fördomar vid rekrytering utgör ett stort problem för utlandsfödda personer i Sverige (Bromé m fl, 1996; Integrationsverket, 2006). För att undersöka namnets (utländskt vs. svenskt) betydelse för hur en arbetssökande (kompetent vs. inkompetent) uppfattas fick 116 studenter bedöma fiktiva CV:n. Hälften av försökspersonerna gjorde sina skattningar under omständigheter där jämställdhet betonades och h

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LevMacinstrumentet är ett analysinstrument under utveckling som utnyttjar leviterade droppar på 100 nl ? 2 ?l. Genom att använda leviterade droppar med den omgivande atmosfären som enda kontaktyta, undviker LevMac?en de vanligaste problemen associerade med miniatyrisering av analytiska tekniker, t.ex. kontaminering av provet genom desorption från provbehållaren eller andra gränsytor, adsorption av

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Utvecklingen av elrullstolar har öppnat upp mängder av möjligheter för personer med funktionshinder som påverkar deras förflyttningsförmåga. Hjälpmedel kan underlätta för en person att vara delaktig i meningsfulla aktiviteter. Genom delaktighet skapar vi relationer och hittar mål och mening med livet. Delaktighet förändras med åldern. Syftet med studien var att undersöka hur elrullstolsanvändare u

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The interest for wind power has increased in Sweden. Northern Sweden is especially interesting due to that a new wind resource map shows that there are areas in northern Sweden that have more potential for wind power than what have been known before. Therefore E.ON Vind is interested in finding out if, and where, there are suitable places for establishment of wind power in northern Sweden. The pur

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This essay is about children who have psychological and social problems. An increase part of children and young people in Sweden describe this kind of difficulties. These problems are at the same time a concern for the school as well as the social welfare. The purpose in this essay is to explore if children with psychological and social problems have the same legal rights to education as other chi

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This thesis investigates the virtual meetings characteristics and policy intervention options in Chinese organizations. Virtual meetings can enable the communication of two or more parities in different locations with assistance of ICT technologies. It is thus possible for virtual meetings to substitute conventional physical face- o-face meetings without business travel and the associated environm

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Vår utgångspunkt är att de attityder som finns gentemot jämställdhetsarbete på en arbetsplats i hög grad bestämmer hur väl arbetet fungerar. Syftet med uppsatsen är således att undersöka attityderna i offentliga organisationer för att se möjligheter och svårigheter i arbetet med att nå jämställdhet. Studien tar sin teoretiska utgångspunkt i demokratiteori och alla människors lika värde, vilket mot