Filtyp
Protein stabilization was achieved through in vivo screening based on the thermodynamic linkage between protein folding and fragment complementation. The split GFP system was found suitable to derive protein variants with enhanced stability due to the correlation between effects of mutations on the stability of the intact chain and the effects of the same mutations on the affinity between fragment
During the last twenty-five years, housing policy in Sweden has radically changed. Once forming a pillar of the comprehensive welfare system, abbreviated ‘the Swedish model’, neoliberal housing politics has established market-governed housing provision with a minimum of state engagement. This shift has had consequences on the social geography of housing conditions. The research reported here analy
The rapidly increasing ability to sequence complete genomes of different microbial species and strains provides us with information regarding their genetic variability. Genetic variability is a mechanism for human pathogens to adapt to and avoid the immune system and to also develop resistance to antibiotics. However, the assessment of the contributions of individual genetic differences to resista
To delineate arsenic (As) safe aquifer(s) within shallow depth, the present study has investigated the shallow hydrostratigraphic framework over an area of 100km(2) at Chakdaha Block of Nadia District, West Bengal. Drilling of 29 boreholes and subsequent hydrostratigraphic modeling has identified three types of aquifer within 50m below ground level (bgl). Aquifer-1 represents a thick paleochannel
Streptococcus spp. are important causes of infective endocarditis but challenging in species identification. This study compared identification based on sequence determination of the rnpB gene with 2 systems of matrix-assisted laser desorption ionization-time of flight mass spectrometry, MALDI Biotyper (Bruker) and VITEK MS IVD (bioMérieux). Blood culture isolates of viridans streptococci from 63
Several psychiatric disorders may be characterized by peripheral telomere shortening. However, it is unclear whether telomere shortening is associated with these psychiatric disorders per se or, rather, with underlying dimensional parameters that are often, but not necessarily, associated with them. We explored the association between dimensional psychopathological measures and telomere length (TL
We examined the effect of 17alpha-ethinylestradiol on immunity of the Western fence lizard, Sceloporus occidentalis. Injection of 17alpha-ethinylestradiol resulted in dose-dependent suppression of peripheral blood leukocyte levels as determined by cell counts, whereas total spleen cell levels were decreased only at higher doses of 17alpha-ethinylestradiol. In contrast, spleen cell proliferation wa
Small intestinal lamina propria (SI-LP) CD103(+) dendritic cells (DCs) are imprinted with an ability to metabolize vitamin A (retinol), a property underlying their enhanced capacity to induce the gut-homing receptors CC chemokine receptor-9 and α4β7 on responding T cells. In this study, we demonstrate that imprinting of CD103(+) DCs is itself critically dependent on vitamin A and occurs locally wi
The outer membrane protein Ail of Yersinia pestis mediates several virulence functions, including serum resistance. Here, we demonstrate that Ail binds C4b-binding protein (C4BP), the primary fluid-phase regulator of the classical and lectin pathways. Non-covalent binding of C4 and C4b to Ail was also observed. C4BP bound to Ail can act as a cofactor to the serine protease factor I (fI) in the cle
Streptococcal toxic shock syndrome is frequently caused by Streptococcus pyogenes of the M1 serotype. The aim of this study was to determine the role of Ras-homologous (Rho)-kinase signaling in M1 protein-provoked lung damage. Male C57BL/6 mice received the Rho-kinase-specific inhibitor Y-27632 before administration of M1 protein. Edema, neutrophil accumulation and CXC chemokines were quantified i
Animals with color vision use color information in intra- and interspecific communication, which in turn may drive the evolution of conspicuous colored body traits via natural and sexual selection. A recent study found that the transparent wings of small flies and wasps in lower-reflectance light environments display vivid and stable structural color patterns, called "wing interference patterns" (
We consider a popular model for long-duration gamma-ray bursts, in which the progenitor star, a stripped helium core, is spun up by tidal interactions with a black hole companion in a compact binary. We perform population synthesis calculations to produce a representative sample of such binaries, and model the effect that the companion has on material that falls back on to the newly formed black h
Antibody-based microarrays are a rapidly evolving affinity-proteomic methodology that recently has shown great promise in clinical applications. The resolution of these proteomic analyses is, however, directly related to the number of data-points, i.e. antibodies, included on the array. Currently, this is a key bottleneck due to limited availability of numerous highly-characterized antibodies. Her
Taking the exhibition "Art in the Streets", which was shown at The Geffen Contemporary at the Museum of Contemporary Art, Los Angeles from 17 April to 8 August, 2011, as its main point of departure, the present article discusses street art's association with the institutions of the art world. "Art in the Streets" sparked a lot of debate before, while and after it took place. The article primarily
Dry stacked light weight concrete block masonry is increasingly used due to its superior productivity compared to standard block masonry. Low bending capacity parallel to the bed joints is however a major drawback. This paper presents the results of an experimental study which has been carried out to improve the bending capacity of dry stacked lightweight concrete block masonry. The effects of fol
The principal noise source for a jet engine is associated with the jet exhaust. The acoustical field that is generated by a jet engine before the take-off is considered. The turbulent jet and the mixing of the hot exhaust gas with the cold surrounding air forms an acoustical source. The compressible Navier-Stokes equations describe both flow field as well as sound generation. The acoustic theory u
This paper presents a pilot study on the prosodic marking of a contrastive topic in Southern Swedish. A test sentence was elicited in three experimental conditions: initial focus; final focus; contrastive topic (initial word) plus focus (final word). F0 patterns were analysed in recordings of 10 speakers. A majority of the speakers distinguished clearly between the conditions, but speakers employe
There are a number of interpretations of the concept “integration” in Product Development (PD). Different sources tend to use different terminologies for describing the same phenomenon of integration, or use the same term for different purposes. This paper strives to capture the essence of the concept of “integration” within a product development project by looking for what is integrated and how i
