Sökresultat

DNA, som nålen i en höstack – vem hittar den först? Inom diagnostiska och forensiska analyser är det viktigt att material undersöks med en metod som är tillförlitlig och som dessutom klarar av att analysera små mängder av materialet. Oavsett om kriminaltekniker hittar blod på en brottsplats, om livsmedlet innehåller smittsamma virus, eller om husdjuret får en allvarlig bakterieinfektion, analyserQuantitative PCR (qPCR) is a commonly used method today in the fields of molecular diagnostics, forensic and food analysis. The real-time measurements of amplification and quantification of specific DNA molecules is one of many reasons why qPCR is the method of choice. However, presence of inhibitory compounds limit this method for example by interacting with either the DNA polymerase or the DNA t

Breast cancer is the most common cancer among women and two thirds of all breast cancers are classified as oestrogen receptor positive. ER is a transcription factor that regulates gene expression and promote cell proliferation that is critical in mammary gland development, and its aberrant activity is commonly present in breast cancer. As a nuclear receptor, ER activity could be diverted by eith

Föreliggande studie handlar om scenskräck och prestationsångest, ett problem som blir allt större i dagens samhälle där kraven ökar. Studien syftar till att uppmärksamma hur nervositet inför framträdanden utgör ett problem också i grundskolan, samt ge råd till grundskollärare hur man kan arbeta förebyggande för att undvika att scenskräck och prestationsångest uppstår. Genom en kvalitativ intervjusThe present study is concerned with performance anxiety, a problem that is increasing in today's society. The study aims to draw attention to how stage fright is a problem also in elementary school, and further wishes to collect advise for how elementary school teachers may work preventively to avoid it occurring. Through a qualitative interview study, I wanted to find out what pedagogical too

Biomolekyler, så som proteiner, produceras genom att DNA som kodar för dessa molekyler tillsätts i celler. Cellerna kan exempelvis vara bakterier, jäst eller från däggdjur. Dessa celler producerar inte enbart de önskade proteinerna utan även andra proteiner som är nödvändiga för att cellerna ska kunna överleva. Som en följd av detta är upprening en av de viktigaste processerna i produktionen av prDownstream processing is an essential part in the production of biomolecules and the optimization of these processes are of great value, since they can be both time consuming and expensive. One common type of enzyme used in different biotechnical approaches is proteases and in this study Protease X was investigated. In order to use the enzyme in a process, a purification procedure needs to be esta

Industrial Internet of Things (IIoT) market is rapidly growing and expected to further expand in the incoming years. To keep up with such a fast pace, new wireless technologies need to be developed to accommodate the huge number of devices expected to connect to the internet. IIoT devices are designed to be low cost devices which operate in low power mode to provide long battery life time. In orde

I en turbin låter man varma och trycksatta gaser expandera. Energin som turbinen fångar upp kan exempelvis omvandlas i generator till elektrisk energi. För att utveckla en välfungerande och bränsleeffektiv turbin krävs god kunskap i termodynamik och aerodynamik. De beräkningar som krävs för att på förhand förutspå en gasturbins prestanda är omfattande och måste utföras med hjälp av datorsimuleringThis paper is the result of a masters thesis with the ambition to create a 1D reduced flow path turbine expansion code with the programming language Python. The work was done at the Department of Energy Sciences at the Faculty of Engineering at Lund University during Spring and Summer of 2018. The work culminated in a run able code, however highly untested and in need of further work. The code is

Platsen är gränslandet mellan stadskärnan i Norrköping, Motala ström och den tilltänkta expansionen av staden i form av en ny stadsdel vid ”Strömmens” norra kaj, Inre hamnen. En plats med potential att knyta samman stad och stadsdel, men framför allt möjligheten att tillföra en bebyggelse med variationsrika rumsligheter. Syftet med denna rapport, ”Strömstaden”, är att utforska en hypotes i form av

Architectural education is most of the time based on architectural tasks with little connection to real circumstances of a building process. The projects focuses on design and the result of the projects is abstractions of the reality, such as drawings, diagrams and models. Architecture through practice aims to explore parts of the architectural practice which we usually don’t have to think about i

A method is implemented wherein numerical approximations to the ground and first few excited states of a quantum mechanical N -body 1D harmonic oscillator are found through variational methods, representing the states as a linear combination of normalized pseudo-states which are themselves linear combinations of non-orthogonal Slater determinants. These states are then used as a low energy basis f

Motivation: Large-scale phylogenetic analyses rely on orthology inference to curate sets of sequences related by speciation rather than gene duplication. Graph-based ‘orthology’ inference approaches cluster sequences together based on an all-versus-all BLAST, followed by filtering by hit fraction, Markov clustering, or both, but the output of such approaches often contains paralogous sequences. Tr

Internet of Things (IoT) is a network constituted by uniquely identifiable commodity objects or devices equipped with some sensing system. To achieve high data rates required for IoT devices, a modern wireless communication system, known as Advanced Antenna Systems(AAS), leads to significant improvement, by increasing not only the data rate but also spectrum efficiency and the channel capacity. A Internet of Things (IoT) is network constituted by day-to-day objects equipped with some sensing system. These objects can also communicate with other objects for data exchange, wirelessly. This calls for a need of better wireless communication systems. New techniques using the 4G (Long-Term Evolution) have been developed to solve this problem, known as Advanced Antenna System (AAS). This new tech

Technology utilisation and implementation of new technologies are critical success factors for humanitarian supply chains and the possibility to improve humanitarian operations by the use of technology has been gaining increasing attention, especially since the 2010 Haiti earthquake. Despite this increase, the theory on emerging technologies within the humanitarian supply chain is still very limit

Purpose: The aim of this study is to explore the effect of changes within traditional production methods, caused by legitimacy crises, on brand authenticity. Design/Methodology/Approach: This research is based on a multi-case study within the leather, ivory and fine wine industry. To evaluate the purpose of this study, literature as same as primary data, in form of a qualitative research, was use

DNA barcodes carry coarse-grained genetic information of DNA sequences taken from a genome. Potential applications include bacteriology, medical diagnosis and taxonomy. However, the current state-of-the-art tools for extracting DNA molecules from cells provide only fragmented pieces of chromosomal DNA. As a consequence, also DNA barcodes are fragmented. This calls for the development of complement

Purpose: Analyzing the outcomes of the Swedish Elections held in September 2018, in order to understand the brand building process in the political context and to find those factors able to shape the success of a party. Methodology: Literature review, case study, questionnaire Findings: The respondents display a high level of trust for their favorite political parties, but lower levels of involv

The harvest mouse Micromys minutus has, through nest findings, been documented to live in wetlands in tall sedges and grasses in Central Europe. However, there is very little information on the type of habitat this species uses outside of nesting, because this rodent is difficult to capture in ordinary trapping set-ups. In France and Switzerland, the harvest mouse populations have decreased strong

The research field of middle management within elderly care in Sweden is fairly unexplored. Even more so, there is little to no research on communication or communicative leadership and its influence on middle management within elderly care. Therefore, this study aims to investigate managers perspective on communication by interviewing middle managers working with elderly care. Furthermore, the st

The concept of brand identity has gained increased interest in recent years from both researchers and practitioners. Despite that, there have been no studies so far linking brand identity to brand experience. The aim of this paper is to investigate how a brand’s identity is transferred in brand experience through in-store and online channels, namely the website and social media (Instagram and Face

Syftet med studien är att undersöka hur implementeringen av byggherredrivna detaljplaneprocesser har fungerat i de kommuner som undersökts. Studien visar på att det inte finns någon större skillnad när det gäller kostnad, tid eller kvalitet vid en jämförelse av en byggherredriven och en traditionellt driven detaljplan. Studien visar även på att en byggherredriven detaljplan är mer genomförbar än The purpose of the study is to investigate how the implementation of local plans produced by the developer have worked in the investigated municipalities. The study shows that there is no major difference in costs, time or quality in between a local plan produced by the developer or the municipality. The study shows that a local plan produced by the developer is easier to implement then a plan pr