Nanoparticle-supported temperature responsive polymer brushes for affinity separation of histidine-tagged recombinant proteins
We developed a modular approach for the preparation of nanoparticle-supported polymer brushes carrying repeating iminodiacetate units for affinity separation of histidine-tagged recombinant proteins. The nanoparticle-supported polymer brushes were prepared via the combination of surface-initiated atom transfer radical polymerization with Cu(I)-catalyzed azide–alkyne cycloaddition reaction. The nan
