Monitoring monohydroperoxides in docosahexaenoic acid using high-performance liquid chromatography
The oxidation of free DHA has been investigated with respect to monohydroperoxides and polyhydroperoxides, which were analyzed with a novel HPLC method. The temperature and physical system, i.e., bulk and liposome, were varied. We have also studied the effects of antioxidants Such as alpha-tocopherol, ascorbic acid, and juice from sea buckthorn on DHA. The HPLC method, which was performed isocrati