Hemoglobin and Immunoglobulin G Inhibition Mechanisms in Diagnostic qPCR
Blood samples are extensively used in clinical and forensic fields for genetic material analysis using real-time PCR. Although PCR is a well-established method distinguished for its rapid and sensitive molecular diagnosis, blood components co- purified with nucleic acids have shown to inhibit amplification and detection of PCR products. In this study, IgG and hemoglobin were investigated for their