Mycobacterium tuberculosis is dependent on cysteine biosynthesis, and reduced sulfur compounds such as mycothiol synthesized from cysteine serve in first-line defense mechanisms against oxidative stress imposed by macrophages. Two biosynthetic routes to L-cysteine, each with its own specific cysteine synthase (CysK1 and CysM), have been described in M. tuberculosis, but the function of a third put

The transpeptidase LtdMt2 catalyzes the formation of the (3-3) cross-links characteristic of the peptidoglycan layer in the Mycobacterium tuberculosis cell wall. Bioinformatics analysis suggests that the extramembrane part of the enzyme consists of three domains: two smaller domains (denoted as A and B domains) and a transpeptidase domain (the C domain) at the C-terminus. The crystal structures of

Effective treatment of tuberculosis is frequently hindered by the emerging antimicrobial resistance of Mycobacterium tuberculosis. The present study evaluates monocyclic β-lactam compounds targeting the mycobacterial cell wall remodeling. Novel N-thio-β-lactams were designed, synthesized, and characterized on the L,D-transpeptidase-2, a validated target in M. tuberculosis. The candidates were eval

RipA plays a vital role during cell division of Mycobacterium tuberculosis by degrading the cell wall peptidoglycan at the septum, allowing daughter cell separation. The peptidoglycan degrading activity relies on the NlpC/P60 domain, and as it is potentially harmful when deregulated, spatial and temporal control is necessary in this process. The N-terminal domain of RipA has been proposed to play

β-lactam antibiotics represent a novel direction in the chemotherapy of tuberculosis that brings the peptidoglycan layer of the complex mycobacterial cell wall in focus as a therapeutic target. Peptidoglycan stability in Mycobacterium tuberculosis, especially during infection, relies on the nonconventional peptide cross-links formed by l,d-transpeptidases. These enzymes are known to be inhibited b

Mental fatigue has attracted much attention from researchers as it plays a key role in performance efficiency and safety situations. Functional connectivity analysis using graph theory is an effective method for revealing changes in cognition resources influenced by mental fatigue. Previous studies have revealed that functional networks are dynamically reorganized. Therefore, it is critical to exp

The present invention relates to a method of isolating a desired nucleic acid from a biological solution, which method comprises to selectively precipitate the desired nucleic acid by adding a polycationic precipitating agent to the solution and allowing it to form a complex with said nucleic acid, wherein the precipitating agent is a highly charged linear polymer that comprises quaternary amino g

A separation agent composed of a water unsoluble solid substance with a covalently bound lectin. Said solid substance is structurally composed of a water unsoluble silica or silicate material as basic material and, via a linkage to silicon atoms in the silica or silicate material, a covalently bound lectin.

A chromatographic separation agent composed of a water unsoluble solid substance showing dihydroxybo ryl groups. Said solid substance is structurally composed of a water unsoluble silica or silicate material as basic structure and, via a linkage to silicon atoms in the silica or silicate material, covalently bound dihydrox yboryl groups in acid, salt or complex form.

An agent for chromatographic separation of mixtures of molecules consists of fibres of inorganic or organic material at least the inner core of each fibre being solid and non-porous.

Review of Marketing Death: Culture and the Making of a Life Insurance Market in China by Cheris Shun-ching Chan (Oxford, UK: Oxford University Press)

Bidrag till specialnummer om praktisk teologi

Background:Prenatal alcohol exposure represents a substantial public health concern as it may lead to detrimental outcomes, including pregnancy complications and fetal alcohol spectrum disorder. Although UK national guidance recommends abstaining from alcohol if pregnant or planning a pregnancy, evidence suggests that confusion remains on this topic among members of the public, and little is known

Vietnam faces a heightened susceptibility to natural disasters stemming from climate change, yet the impact of these disasters varies markedly across its regions. Consequently, it is imperative to scrutinize the climatic factors affecting the regional economy, with a particular dearth of research addressing the nexus between natural threats and the economies of the Mekong River Delta and the South

The development of a decarbonised food industry will depend on a sustainable agricultural system where embodied food product greenhouse gas emissions (GHG) can be associated with agricultural production. The results presented demonstrate how physically mapping agri-production provides regional carbon footprints so practice that reduces GHG emissions become strategic rather than random. Different a

The complex nature of climate change requires action at different scales and in different ways, but questions remain around how to produce climate action that is both multi-scalar and joined up. Here we explore this question by adopting a relational approach to studying climate action by faith-based actors, who increasingly play an active role campaigning on climate action. Drawing on qualitative

Collaborative spaces such as fab labs, incubators, and coworking spaces have become a worldwide phenomenon based on their promise of fostering creativity and entrepreneurship. Mature, large organizations have also tried to benefit from this by creating such spaces in-house. This paper studies a failed attempt to create one such space in a large bureaucratic organization. Our study shows that succe