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Catalytically Active MIP Architectures

Molecularly imprinted polymers (MIPs) have been developed mostly for the specific binding of the target, mimicking the function of antibodies. In addition to these " plastic antibodies," completely synthetic catalysts have been created that mimic substrate conversion by enzymes. In this chapter, the following examples will be presented: (1) an esterolytic MIP for phenolic esters that is prepared u

The formation of doctoral education

This report has been translated from Swedish and adapted for an international audience. The original report, Leadership for quality in doctoral education1, was commissioned by the Association of Swedish Higher Education. Here we describe and discuss doctoral education as policy, practice and as an object of knowledge, and highlight a number of questions that are important for the management and le

Film & TV : Aesthetic Interaction

A discussion of inherent limitations in early live television, the methods necessary in live productions and aesthetic consequences that live TV resulted in.

Film and Television in Interaction

The text emphasizes aesthetics in post war American film and is more precisely a study of directors, which started their career within live television and made a transition to cinema. The influence of their television background on their films is central to the text. Contributing factors in this context are changes in the overall technology within TV, changes from live productions to the use of fi

Phosphoproteins and protein kinase activities intrinsic to inner membranes of potato tuber mitochondria

Inside-out submitochondrial particles (IO-SMP) were isolated and purified from potato (Solanum tuberosum L. cv.) tubers. When these IO-SMP were incubated with [γ32P]ATP more then 20 proteins became labelled as a result of phosphorylation. The 32P incorporation was stimulated by the oxidizing reagent ferricyanide. Except for a 17 kDa protein which was phosphorylated only in the absence of divalent

Purification of a serine and histidine phosphorylated mitochondrial nucleoside diphosphate kinase from Pisum sativum

For the first time, to our knowledge, a nucleoside diphosphate kinase (NDPK) has been purified from plant mitochondria (Pisum sativum L.). In intact pea leaf mitochondria, a 17.4-kDa soluble protein was phosphorylated in the presence of EDTA when [γ-32]ATP was used as the phosphate donor. Cell fractionation demonstrated that the 17.4-kDa protein is a true mitochondrial protein, and the lack of acc

Two subunits of the F0F1-ATPase are phosphorylated in the inner mitochondrial membrane

Inside-out submitochondrial particles from potato tuber mitochondria were incubated with [γ-32P]ATP. More than 16 phosphorylated polypeptides were detected by autoradiography on an SDS-gel. Two phosphoproteins, migrating at 22 and 28 kDa, were excised from the SDS-gel, electroeluted, and purified further by anion chromatography. The phosphoproteins were N-terminally sequenced. Over the regions seq

Biological valorization of low molecular weight lignin

Lignin is a major component of lignocellulosic biomass and as such, it is processed in enormous amounts in the pulp and paper industry worldwide. In such industry it mainly serves the purpose of a fuel to provide process steam and electricity, and to a minor extent to provide low grade heat for external purposes. Also from other biorefinery concepts, including 2nd generation ethanol, increasing am

Physicochemical Characterisation of Technical Lignins for Their Potential Valorisation

Lignin, the second most abundant natural polymer, has emerged as a potential alternative material to petroleum-based chemicals and renewable resource for the production of diverse forms of aromatics, biofuels, and bio-based materials. Thus, it is becoming important to understand its structure and properties to provide key features and insights for better/efficient lignin valorisation. In this work

Competence Building: A Systemic Approach to Innovation Policy

The main question that guides this paper is how governments are focusing (and must focus) on competence building (education and training) when designing and implementing innovation policies. With this approach, the paper aims at filling the gap between the existing literature on competences on the one hand, and the real world of innovation policy-making on the other, typically not speaking to each