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Hyphal exploration strategies and habitat modification of an arbuscular mycorrhizal fungus in microengineered soil chips

Arbuscular mycorrhizal fungi (AMF) are considered ecosystem engineers, but the interactions of their mycelium with their immediate surroundings are largely unknown. In this study, we used microfluidic chips, simulating artificial soil structures, to study foraging strategies and habitat modification of Rhizophagus irregularis symbiotically associated to carrot roots. AMF hyphae foraged over long d

The C-terminal domain of the antiamyloid chaperone DNAJB6 binds to amyloid-β peptide fibrils and inhibits secondary nucleation

The DNAJB6 chaperone inhibits fibril formation of aggregation-prone client peptides through interaction with aggregated and oligomeric forms of the amyloid peptides. Here, we studied the role of its C-terminal domain (CTD) using constructs comprising either the entire CTD or the first two or all four of the CTD β-strands grafted onto a scaffold protein. Each construct was expressed as WT and as a

Inequalities in water, sanitation and hygiene : Challenges and opportunities for measurement and monitoring

While there is significant awareness of the importance of addressing water, sanitation and hygiene (WASH) inequalities, measurement continues to present a challenge. Addressing how inequalities are measured, tracked and communicated is fundamental to accelerating progress in ensuring universal WASH coverage and associated benefits. We review how WASH inequalities have been measured and monitored t

Can L2 motivation be modelled as a self-system? A critical assessment

Some two decades ago, Dörnyei (2005) proposed that motivation for L2 learning could be modelled as a self-system. Despite the profound influence of Dörnyei's scholarship and impact of the L2 Motivational Self System (L2MSS) model, the fundamental premise that motivation for L2 learning can constitute a self-system has escaped critical scrutiny. Highlighting how the self-system constitutes a framew

Escape to the future – a qualitative study of physicians’ views on the work environment, education, and support in a digital context

Background: The use of remote services such as video consultations (VCs) has increased significantly in the wake of the COVID-19 pandemic. In Sweden, private healthcare providers offering VCs have grown substantially since 2016 and have been controversial. Few studies have focused on physicians’ experiences providing care in this context. Our aim was to study physicians’ experiences of VCs, focusi

aMeta : an accurate and memory-efficient ancient metagenomic profiling workflow

Analysis of microbial data from archaeological samples is a growing field with great potential for understanding ancient environments, lifestyles, and diseases. However, high error rates have been a challenge in ancient metagenomics, and the availability of computational frameworks that meet the demands of the field is limited. Here, we propose aMeta, an accurate metagenomic profiling workflow for

H2 formation from the E2–E4 states of nitrogenase

Nitrogenase is the only enzyme that can cleave the strong triple bond in N2, making nitrogen available for biological lifeforms. The active site is a MoFe7S9C cluster (the FeMo cluster) that binds eight electrons and protons during one catalytic cycle, giving rise to eight intermediate states E0–E7. It is experimentally known that N2 binds to the E4 state and that H2 is a compulsory biproduct of t

Delayed vascular complication after collagenase injection for Dupuytren disease

Background: Vascular adverse events after collagenase injection for Dupuytren disease are absent in large trials and systematic reviews. The aim of this study is to present a case series of delayed vascular complications after collagenase treatment. Methods: A prospective evaluation of 1181 consecutively treated patients at one orthopedic department identified three patients reporting symptoms of

Urban farming with rooftop greenhouses : A systematic literature review

The environmental impacts of food systems will increase in tandem with rapid urban population growth, which calls for alternative solutions, such as urban agriculture, to reach the United Nations Sustainable Development Goals. Among several urban agriculture systems, rooftop farming and its subset, rooftop greenhouses, are promising technologies. They optimize land use, increase profitability for

Ponds as experimental arenas for studying animal movement : current research and future prospects

Animal movement is a multifaceted process that occurs for multiple reasons with powerful consequences for food web and ecosystem dynamics. New paradigms and technical innovations have recently pervaded the field, providing increasingly powerful means to deliver fine-scale movement data, attracting renewed interest. Specifically in the aquatic environment, tracking with acoustic telemetry now provi

A study on how efficient measures for secondary district heating system performance can be encouraged by motivational tariffs

Background: District Heating (DH) is a technology that provides heating and domestic hot water to buildings and is an important technology for supporting the European energy transition. As such the heating systems increasingly resort to renewable heat sources and waste heat, it is even more important that they operate in the most efficient way possible. DH companies have access to the primary netw

Synthesizing implications of a collaborative project on socially sustainable housing policies for the aging population

Objective: Based on findings from four transdisciplinary original research studies on housing issues for the aging population, whereof three had a particular focus on marginalized groups, we report a co-produced synthesis of implications from a collaborative research project on socially sustainable housing policies. Researchers and non-academic partners in the ongoing Thematic Collaboration Initia

A Computer Program for Assessing Histoanatomical Morphometrics in Ultra-High-Frequency Ultrasound Images of the Bowel Wall in Children : Development and Inter-Observer Variability

Ultra-high-frequency ultrasound (UHFUS) has a reported potential to differentiate between aganglionic and ganglionic bowel wall, referred to as histoanatomical differences. A good correlation between histoanatomy and UHFUS of the bowel wall has been proven. In order to perform more precise and objective histoanatomical morphometrics, the main research objective of this study was to develop a compu

Hybrid elicitation and quantile-parametrized likelihood

This paper extends the application of quantile-based Bayesian inference to probability distributions defined in terms of quantiles of observable quantities. Quantile-parameterized distributions are characterized by high shape flexibility and parameter interpretability, making them useful for eliciting information about observables. To encode uncertainty in the quantiles elicited from experts, we p

Effect of Aurora kinase B on polyploidy and decidualization in mouse uterus

Research question: Decidualization is critical to the establishment of mouse normal pregnancy. The fibroblast-like stromal cells in the process form polyploid multinucleated cells. Aurora kinase B (Aurora B) has previously been shown to regulate polyploidy in various cells. However, whether Aurora B regulates the formation of decidual cell polyploidization and its regulatory mechanisms remain poor

Children with Hirschsprung’s Disease Report Dietary Effects on Gastrointestinal Complaints More Frequently than Controls

Hirschspung’s disease (HD) is a congenital gastrointestinal (GI) disorder frequently accompanied by GI complaints. Despite the lack of evidence regarding whether diet affects GI symptoms, advice on dietary changes is common. The aim was to investigate self-reported dietary effects on GI symptoms, comparing children with HD with healthy children. This was an observational, cross-sectional, self-rep

Portal vein embolization with N-butyl-cyanoacrylate improves liver hypertrophy compared to microparticles – A Swedish multicenter cohort study

Background: An adequate future liver remnant (FLR) is fundamental for major liver resections. To achieve sufficient FLR, portal vein embolization (PVE) may be used. The most effective material for PVE has yet to be determined. The aim of this study was to investigate the differences in FLR growth between n-butyl-cyanoacrylate glue (NBCA) and microparticles. Material/methodsa: retrospective study w

Emotion dysregulation – A bridge between ACE and aggressive antisocial behavior

Purpose: Forensic populations have high prevalence rates of adverse childhood experiences (ACE), but specific pathways from childhood victimization to adult victimizing behavior remain unclear. Difficulties in emotion regulation may prove to be a more proximal risk factor that functions as a pathway between ACE and aggressive antisocial behavior (AAB). In this study, we investigated concurrent ass