Capillary gas chromatography using electron capture or selected ion monitoring detection for the determination of muramic acid, diaminopimelic acid and the ratio of d/l-alanine in bacteria
Gas chromatographic analyses of muramic acid, diaminopimelic acid and D-alaline, which are specific components of the bacterial cell wall, have been performed using electron capture or selected ion monitoring detection. Intact cells or peptidogylycan preparations were hydrolyzed in HCl and DCl. After purification by cation exchange chromatography, followed by conversion to the N-heptafluobutyrliso